Results 1 to 10 of about 350 (116)

VNAR: shark single-domain antibodies for the new era of medical biotechnology [PDF]

Frontiers in Immunology
Shark-derived single-domain antibodies, known as VNARs, represent unique and advanced tools in medical biotechnology. Recognized for their small size, simple structure, and exceptional stability, VNARs can access cryptic epitopes that are inaccessible to
Alexis M Kalergis, Margarita K Lay, Augusto Manubens   +2 more
exaly   +7 more sources

A novel PD-L1-targeted shark VNAR single-domain-based CAR-T cell strategy for treating breast cancer and liver cancer [PDF]

Molecular Therapy - Oncolytics, 2022
Chimeric antigen receptor (CAR)-T cell therapy shows excellent potency against hematological malignancies, but it remains challenging to treat solid tumors, mainly because of a lack of appropriate antigenic targets and an immunosuppressive tumor ...
Chi-Ping Day, Glenn Merlino, Mitchell Ho
exaly   +7 more sources

Synthetic libraries of shark vNAR domains with different cysteine numbers within the CDR3 [PDF]

PLoS ONE, 2019
The variable domain of New Antigen Receptors (vNAR) from sharks, present special characteristics in comparison to the conventional antibody molecules such as: small size (12-15 kDa), thermal and chemical stability and great tissue penetration, that makes
Alexandra Rucavado, Alexei Licea-Navarro, Teresa Escalante   +2 more
exaly   +5 more sources

Identification of nurse shark VNAR single-domain antibodies targeting the spike S2 subunit of SARS-CoV-2

FASEB Journal, 2023
Shark VNAR nanobodies targeting the S2 subunit of SARS‐CoV‐2 spike protein were identified from a naïve nurse shark VNAR phage display library by phage panning. The S2A9 VNAR showed the best neutralization activity against the original pseudotyped virus and was further characterized.
Hyung Joon Kwon, Hang Xie, Mingqian Feng
exaly   +4 more sources

In vitro selection of VNAR antibodies using bioinformatics-based library design and cDNA display [PDF]

Biotechnology Reports
Variable domains of new antigen receptors (VNARs) derived from shark immunoglobulin new antigen receptors (IgNARs) have gained attention as promising single-domain antibody modalities owing to their compact structures, structural stability, and unique ...
Hiroki Anzai, Takuya Terai, Susumu Mitsuyama, Ryo Matsunaga, Kenwa Tamagawa, Issei Yamaguchi, D.P.N. De Silva, Shigehiro Kuraku, Kouhei Tsumoto, Robert E. Campbell, Shuichi Asakawa   +10 more
doaj   +2 more sources

VNAR development through antigen immunization of Japanese topeshark (Hemitriakis japanica) [PDF]

Frontiers in Bioengineering and Biotechnology, 2023
The VNAR (Variable New Antigen Receptor) is the smallest single-domain antibody derived from the variable domain of IgNAR of cartilaginous fishes. Despite its biomedical and diagnostic potential, research on VNAR has been limited due to the difficulties ...
Hiroyuki Takeda, Tatsuhiko Ozawa, Tatsuhiko Ozawa, Hiroki Zenke, Yoh Ohnuki, Yuri Umeda, Wei Zhou, Honoka Tomoda, Akihiko Takechi, Kimiyoshi Narita, Takaaki Shimizu, Takuya Miyakawa, Yuji Ito, Tatsuya Sawasaki   +13 more
doaj   +2 more sources

First pan-specific vNAR against human TGF-β as a potential therapeutic application: in silico modeling assessment [PDF]

Scientific Reports, 2023
Immunotherapies based on antibody fragments have been developed and applied to human diseases, describing novel antibody formats. The vNAR domains have a potential therapeutic use related to their unique properties.
Mirna Burciaga-Flores, Ana Laura Márquez-Aguirre, Salvador Dueñas, Jahaziel Gasperin-Bulbarela, Alexei F. Licea-Navarro, Tanya A. Camacho-Villegas   +5 more
doaj   +2 more sources

Novel Approach for Obtaining Variable Domain of New Antigen Receptor with Different Physicochemical Properties from Japanese Topeshark (Hemitriakis japanica) [PDF]

Marine Drugs, 2023
Diverse candidate antibodies are needed to successfully identify therapeutic and diagnostic applications. The variable domain of IgNAR (VNAR), a shark single-domain antibody, has attracted attention owing to its favorable physicochemical properties.
Tomofumi Nakada-Masuta, Hiroyuki Takeda, Kazuhisa Uchida   +2 more
doaj   +2 more sources

Generation of shark single-domain antibodies as an aid for Cryo-EM structure determination of membrane proteins: Use hyaluronan synthase as an example [PDF]

Journal of Structural Biology: X
In cartilaginous fish, the immunoglobulin new antigen receptor (IgNAR) is naturally devoid of light chains. The variable regions of IgNAR (VNARs) are solely responsible for antigen recognition, similar to VHHs (variable domain of the heavy chain of heavy-
Penghui Deng, Xiaoyue Zhang, Jianqing Wen, Mingce Xu, Pengwei Li, Hao Wang, Yunchen Bi   +6 more
doaj   +2 more sources

The characterization of variable new antigen receptors targeting FAP isolated from a novel immunized library [PDF]

Communications Biology
Cancer-associated fibroblasts (CAFs) in the stroma of solid tumors promote an immunosuppressive tumor microenvironment (TME) that drives resistance to therapies.
Gihan S. Gunaratne, Joseph P. Gallant, Kendahl L. Ott, Payson L. Broome, Sasha Celada, Jayden L. West, Jason C. Mixdorf, Eduardo Aluicio-Sarduy, Jonathan W. Engle, Eszter Boros, Labros Meimetis, Joshua M. Lang, Shuang G. Zhao, Reinier Hernandez, David Kosoff, Aaron M. LeBeau   +15 more
doaj   +2 more sources