In Vitro Maturation of a Humanized Shark VNAR Domain to Improve Its Biophysical Properties to Facilitate Clinical Development [PDF]
Frontiers in Immunology, 2017 Molecular engineering to increase the percentage identity to common human immunoglobulin sequences of non-human therapeutic antibodies and scaffolds has become standard practice.
John Steven +11 more
doaj +12 more sources
A novel PD-L1-targeted shark VNAR single-domain-based CAR-T cell strategy for treating breast cancer and liver cancer [PDF]
Molecular Therapy: Oncolytics, 2022 Chimeric antigen receptor (CAR)-T cell therapy shows excellent potency against hematological malignancies, but it remains challenging to treat solid tumors, mainly because of a lack of appropriate antigenic targets and an immunosuppressive tumor ...
Dan Li +6 more
doaj +7 more sources
Unleashing the power of shark variable single domains (VNARs): broadly neutralizing tools for combating SARS-CoV-2 [PDF]
Frontiers in Immunology, 2023 The pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) generated a joint global effort to develop vaccines and other treatments that could mitigate the negative effects and the rapid spread of the virus.
Olivia Cabanillas-Bernal +4 more
doaj +7 more sources
Construction and application of a large capacity VNAR library from the whitespotted bamboo shark (Chiloscyllium playgiosum) [PDF]
Acta Pharmaceutica Sinica BFifty whitespotted bamboo sharks (Chiloscyllium playgiosum) of both sexes were used to establish a large capacity variable domain of the new antigen receptor (VNAR) library with a total capacity of over 109 colony-forming units (CFU).
Hao Li +9 more
doaj +5 more sources
Single domain shark VNAR antibodies neutralize SARS-CoV-2 infection in vitro. [PDF]
FASEB J, 2021 AbstractSingle domain shark VNAR antibodies can offer a viable alternative to conventional Ig-based monoclonal antibodies in treating COVID-19 disease during the current pandemic. Here we report the identification of neutralizing single domain VNAR antibodies selected against the SARS-CoV-2 spike protein derived from the Wuhan variant using phage ...
Gauhar A +8 more
europepmc +8 more sources
Synthetic libraries of shark vNAR domains with different cysteine numbers within the CDR3. [PDF]
PLoS ONE, 2019 The variable domain of New Antigen Receptors (vNAR) from sharks, present special characteristics in comparison to the conventional antibody molecules such as: small size (12-15 kDa), thermal and chemical stability and great tissue penetration, that makes
Olivia Cabanillas-Bernal +5 more
doaj +5 more sources
Isolation and Characterization of Targeting-HBsAg VNAR Single Domain Antibodies from Whitespotted Bamboo Sharks (Chiloscyllium plagiosum) [PDF]
Marine Drugs, 2023 Immunoglobulin new antigen receptor (IgNAR) is a naturally occurring antibody that consists of only two heavy chains with two independent variable domains.
Xierui Jiang +3 more
doaj +4 more sources
Expression Analysis of Heavy-Chain-Only Antibodies in Cloudy Catshark and Japanese Bullhead Shark [PDF]
Marine DrugsHeavy chain-only antibodies in sharks are called immunoglobulin new antigen receptors (IgNAR), consisting of one variable region (VNAR) and five constant regions (C1-C5).
Reo Uemura +8 more
doaj +5 more sources
Preparation of pH-sensitive nanogels bioconjugated with shark antibodies (VNAR) for targeted drug delivery with potential applications in colon cancer therapies. [PDF]
PLoS ONECancer is the second leading cause of death worldwide. To combat this disease, novel and specialized therapeutic systems are urgently needed. This is the first study to explore a system that combines shark variable domain (Fv) of new antigen receptor ...
Lizbeth A Manzanares-Guevara +6 more
doaj +6 more sources
Identification of nurse shark V<sub>NAR</sub> single-domain antibodies targeting the spike S2 subunit of SARS-CoV-2. [PDF]
FASEB J, 2023 Shark VNAR nanobodies targeting the S2 subunit of SARS‐CoV‐2 spike protein were identified from a naïve nurse shark VNAR phage display library by phage panning. The S2A9 VNAR showed the best neutralization activity against the original pseudotyped virus and was further characterized.
Buffington J +7 more
europepmc +2 more sources