Results 31 to 40 of about 335 (107)

Ancient species offers contemporary therapeutics: an update on shark V_NAR single domain antibody sequences, phage libraries and potential clinical applications. [PDF]

Antib Ther, 2020
ABSTRACTThe antigen binding variable domain (VNAR) of the shark immunoglobulin new antigen receptor (IgNAR) evolved approximately 500 million years ago and it is one of the smallest antibody fragments in the animal kingdom with sizes of 12–15 kDa.
English H, Hong J, Ho M.
europepmc   +4 more sources

Shark New Antigen Receptor (IgNAR): Structure, Characteristics and Potential Biomedical Applications

Cells, 2021
Shark is a cartilaginous fish that produces new antigen receptor (IgNAR) antibodies. This antibody is identified with a similar human heavy chain but dissimilar sequences.
Salma Nassor Juma, Xiaoxia Gong, Sujie Hu, Zhengbing Lv, Jianzhong Shao, Lili Liu, Guiqian Chen   +6 more
doaj   +1 more source

Identification of Anti-TNFα VNAR Single Domain Antibodies from Whitespotted Bambooshark (Chiloscyllium plagiosum)

Marine Drugs, 2022
Tumor necrosis factor α (TNFα), an important clinical testing factor and drug target, can trigger serious autoimmune diseases and inflammation. Thus, the TNFα antibodies have great potential application in diagnostics and therapy fields.
Linfei Zhao, Mingliang Chen, Xiaona Wang, Shoukai Kang, Weiwei Xue, Zengpeng Li   +5 more
doaj   +1 more source

Marine-derived microbes and molecules for drug discovery

Inflammation and Regeneration, 2022
Increasing attention has been paid to marine-derived biomolecules as sources of therapeutics for autoimmune diseases. Nagasaki Prefecture has many islands and is surrounded by seas, straits, gulfs, bays, and coves, giving it the second longest coastline ...
Yoshimasa Tanaka, Megumi Nishikawa, Kaho Kamisaki, Saki Hachiya, Moeka Nakamura, Takahiro Kuwazuru, Susumu Tanimura, Kiyoshi Soyano, Kohsuke Takeda   +8 more
doaj   +1 more source

Shark-derived single-domain antibodies for the detection of decapod iridescent virus 1

Aquaculture Reports, 2023
Decapod iridescent virus 1 (DIV1) has emerged as a novel threat to shrimp farming. The development of a rapid, convenient, and accurate method for diagnosing DIV1 is urgently needed, serving as a key factor in the reduction of the risk of viral outbreaks.
Jinlin Chen, Wei Wang, Qin Zheng, Yunying Cheng, Qian Zhang, Shenfei Jiang, Jianming Chen   +6 more
doaj   +1 more source

Identification and characterization of IgNAR and VNAR repertoire from the ocellate spot skate (Okamejei kenojei)

Frontiers in Marine Science, 2023
Elasmobranchs are crucial for comparative studies of evolution, as they belong to the most ancient vertebrate lineages that survived numerous extinction events and persist until today.
Jianqing Wen, Jianqing Wen, Jinyu Gong, Jinyu Gong, Pengwei Li, Penghui Deng, Penghui Deng, Mengsi Sun, Yujie Wu, Chenxi Tian, Chenxi Tian, Hao Wang, Yunchen Bi, Yunchen Bi, Yunchen Bi   +14 more
doaj   +1 more source

Mechanisms of SARS-CoV-2 neutralization by shark variable new antigen receptors elucidated through X-ray crystallography

Nature Communications, 2021
Shark antibodies (Variable New Antigen Receptors, VNARs) are the smallest naturally occurring antibody fragments. Here, the authors screen a VNAR phage display library against the SARS-CoV2 receptor binding domain (RBD) and identify VNARs that neutralize
Obinna C. Ubah, Eric W. Lake, Gihan S. Gunaratne, Joseph P. Gallant, Marie Fernie, Austin J. Robertson, Jonathan S. Marchant, Tyler D. Bold, Ryan A. Langlois, William E. Matchett, Joshua M. Thiede, Ke Shi, Lulu Yin, Nicholas H. Moeller, Surajit Banerjee, Laura Ferguson, Marina Kovaleva, Andrew J. Porter, Hideki Aihara, Aaron M. LeBeau, Caroline J. Barelle   +20 more
doaj   +1 more source

Brain delivery of biologics using a cross‐species reactive transferrin receptor 1 VNAR shuttle

The FASEB Journal, Volume 34, Issue 10, Page 13272-13283, October 2020., 2020
Abstract Transferrin receptor 1 (TfR1) mediated transcytosis is an attractive strategy to enhance brain uptake of protein drugs, but translation remains a challenge. Here, a single domain shark antibody VNAR fragment (TXB2) with similar affinity to murine and human TfR1 was used to shuttle protein cargo into the brain. TXB2 was fused to a human IgG1 Fc
Dag Sehlin, Pawel Stocki, Tobias Gustavsson, Greta Hultqvist, Frank S. Walsh, J. Lynn Rutkowski, Stina Syvänen   +6 more
wiley   +1 more source

Screening and Characterization of Shark-Derived VNARs against SARS-CoV-2 Spike RBD Protein

International Journal of Molecular Sciences, 2022
The receptor-binding domain (RBD) of the SARS-CoV-2 spike protein is the major target for antibody therapeutics. Shark-derived variable domains of new antigen receptors (VNARs) are the smallest antibody fragments with flexible paratopes that can recognize protein motifs inaccessible to classical antibodies. This study reported four VNARs binders (JM-2,
Yu-Lei Chen, Jin-Jin Lin, Huan Ma, Ning Zhong, Xin-Xin Xie, Yunru Yang, Peiyi Zheng, Ling-Jing Zhang, Tengchuan Jin, Min-Jie Cao   +9 more
openaire   +2 more sources

Human TNF cytokine neutralization with a vNAR fromHeterodontus franciscishark: A potential therapeutic use [PDF]

mAbs, 2013
The therapeutic use of single domain antibodies (sdAbs) is a promising new approach because these small antibodies maintain antigen recognition and neutralization capacity, have thermal and chemical stability and have good solubility. In this study, using phage display technology, we isolated a variable domain of a IgNAR (vNAR) from a Heterodontus ...
Tanya, Camacho-Villegas, Teresa, Mata-Gonzalez, Jorge, Paniagua-Solis, Edna, Sanchez, Alexei, Licea   +4 more
openaire   +2 more sources