Construction and next-generation sequencing analysis of a large phage-displayed VNAR single-domain antibody library from six naïve nurse sharks [PDF]
Antibody Therapeutics, 2018 ABSTRACT Background Shark new antigen receptor variable domain (VNAR) antibodies can bind restricted epitopes that may be inaccessible to conventional antibodies. Methods Here, we developed a library construction method based on ...
Feng, Mingqian +8 more
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Single Domain Antibodies as New Biomarker Detectors
Diagnostics, 2017 Biomarkers are defined as indicators of biological processes, pathogenic processes, or pharmacological responses to a therapeutic intervention. Biomarkers have been widely used for early detection, prediction of response after treatment, and for ...
Chiuan Herng Leow +5 more
doaj +1 more source
Efficient Construction and Effective Screening of Synthetic Domain Antibody Libraries
Methods and Protocols, 2019 Phage display is a powerful technique for drug discovery in biomedical research in particular for antibody libraries. But, several technical challenges are associated with the selection process.
Arghavan Solemani Zadeh +4 more
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Enhancing Stability of Camelid and Shark Single Domain Antibodies: An Overview
Frontiers in Immunology, 2017 Single domain antibodies (sdAbs) are gaining a reputation as superior recognition elements as they combine the advantages of the specificity and affinity found in conventional antibodies with high stability and solubility.
Ellen R. Goldman +3 more
doaj +1 more source
Identification of nurse shark V<sub>NAR</sub> single-domain antibodies targeting the spike S2 subunit of SARS-CoV-2. [PDF]
FASEB J, 2023 Buffington J +7 more
europepmc +1 more source
, 2018 Additional file 1. PCR amplification of randomly selected plaques from the VNAR domains primary library using T7SelectUP (For) and T7SelectDOWN (Rev) sequencing primers. Lane M represents 100Â bp ladder; lane 1â 20 represents insert of single plaque; lane â ve represents negative control (PCR product with no cDNA template).
Leow, Chiuan +4 more
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, 2018 Additional file 2. Deduced amino acid sequences in hypervariable regions of VNAR clones targeting to three malaria biomarkers. The non-canonical cysteine residue is highlighted in red colour. This table also indicates the length of CDR3, number of cysteine residue, and type of IgNAR family for each clone.
Leow, Chiuan +4 more
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Humanized shark variable new antigen receptors (VNARs) [PDF]
Science-Business eXchange, 2013 openaire +1 more source
Development and application of a VNAR-based detection nanobody for avian influenza virus H9N2. [PDF]
Front ImmunolYe H +9 more
europepmc +1 more source
Chimeric Peptides from Californiconus californicus and Heterodontus francisci with Antigen-Binding Capacity: A Conotoxin Scaffold to Create Non-Natural Antibodies (NoNaBodies). [PDF]
Toxins (Basel), 2023 Dueñas S +11 more
europepmc +1 more source