Results 111 to 120 of about 79,021 (275)
Zhongguo shiyan zhenduanxue, 2013 目的构建人Shh基因过表达慢病毒载体,转染293T细胞,建立稳定转染人Shh基因的293T的细胞系。方法设计引物引入AgeⅠ酶切位点,使用PCR方法从质粒中扩增Shh基因的编码区序列,对所扩增出的目的片段回收纯化。将AgeⅠ内切酶消化后目的片段交换连接入pGC-FU载体,构建Shh慢病毒表达载体pGC-FU-Shh。酶切验证并测序正确后,将质粒pGC-FU-Shh与携带GFP的慢病毒辅助包装载体共转染293T细胞,荧光显微镜检测慢病毒载体转染细胞的结果,重组质粒pGC-FU-Shh的测序,Western
边学海 +6 more
doaj
, 2015 Table 1-Phosphatome Screen-Shh Signaling. The individual genes tested in primary screen for phosphatases involved in Shh signaling. Gene symbol, gene name and identifier and the target sequence are shown.
Rosalind Segal (682740) +1 more
core +1 more source
The FGF/FGFR System in the Biology and Therapeutic Landscape of Pediatric CNS Tumors
Medicinal Research Reviews, EarlyView.ABSTRACT Central nervous system (CNS) tumors are the most common solid malignancies in children, comprising a highly heterogeneous group of neoplasms defined by distinct molecular alterations and clinical behaviors. Advances in molecular genetics have underscored the relevance of specific signaling pathways in driving pediatric tumorigenesis, among ...
Serena Filiberti +6 more
wiley +1 more source
Identification of Novel Interacting Proteins of FUZ and GPR161
PROTEOMICS, EarlyView.ABSTRACT Protein–protein interactions are central to the dynamic regulation of signaling pathways and provide critical insight into the cellular mechanisms underlying human disease. Our previous study demonstrated biochemical and genetic interactions between FUZ and GPR161 in sonic hedgehog signaling during spinal neural tube development. In this study,
Gabriella Salazar +3 more
wiley +1 more source
Phytotherapy Research, EarlyView.Schematic overview of the multi‐target and multi‐pathway mechanisms of PDBI in the prevention and management of hypertension. ABSTRACT Hypertension remains a major global risk factor for cardiovascular diseases, yet current treatments face challenges such as suboptimal blood pressure control and inadequate organ protection.
Qiqi Deng +6 more
wiley +1 more source
Population MedicineIntroduction The aim of the study was to assess the prevalence and associated factors of hand hygiene (HH) and oral hygiene (OH) behavior in adolescents in Central America.
Supa Pengpid, Karl Peltzer
doaj +1 more source
Phytotherapy Research, EarlyView.Pancreatic cancer, particularly pancreatic ductal adenocarcinoma, is aggressive and often diagnosed late, making treatment challenging. This review highlights the potential of phytochemicals, such as curcumin and quercetin, as multitarget agents that influence key carcinogenic pathways. These compounds exhibit anticancer effects by inhibiting processes
Md. Rezaul Islam +7 more
wiley +1 more source
The British Journal of Sociology, EarlyView.ABSTRACT As digital technologies become embedded in everyday life, interpersonal violence increasingly takes place in cyberspace. Existing studies have focused on how states, experts, and the media define what counts as cyber violence and abuse. Less attention has been paid to how ordinary people interpret and contest these definitions and how their ...
Susanne Y. P. Choi +4 more
wiley +1 more source
Acta Neuropathologica CommunicationsSonic hedgehog subgroup of medulloblastoma (SHH-MB) is characterized by aberrant activation of the SHH signaling pathway. An inhibition of the positive SHH regulator Smoothened (SMO) has demonstrated promising clinical efficacy. Yet, primary and acquired
Foteini Tsiami +13 more
doaj +1 more source
Classification of videolaryngoscopy: a systematic review
Anaesthesia, EarlyView.Summary Introduction Structured classification and consistent documentation of videolaryngoscopy findings are essential for future airway planning. Although numerous classification tools for communication have been proposed, their accuracy and effectiveness remain uncertain.
Catharina Grün +8 more
wiley +1 more source