Results 11 to 20 of about 590,891 (162)
Stabilization of a Broadly Neutralizing Anti-Chikungunya Virus Single Domain Antibody
Frontiers in Medicine, 2021 A single domain antibody (clone CC3) previously found to neutralize a vaccine strain of the chikungunya virus (PRNT50 = 2. 5 ng/mL) was found to be broadly neutralizing.
Jinny L. Liu +9 more
doaj +1 more source
Single Domain Antibody application in bacterial infection diagnosis and neutralization
Frontiers in Immunology, 2022 Increasing antibiotic resistance to bacterial infections causes a serious threat to human health. Efficient detection and treatment strategies are the keys to preventing and reducing bacterial infections. Due to the high affinity and antigen specificity,
Qian Qin +11 more
doaj +1 more source
Single-domain antibody screening by isPLA-seq [PDF]
Life Science Alliance, 2021 Single-domain antibody (sdAb) holds the promising strategies for diverse research and translational applications. Here, we describe a method for the adaptation of the in situ proximity ligation assay (isPLA) followed by sequencing (isPLA-seq) to facilitate screening of a high-sensitive, high-throughput sdAb library for a given protein at subcellular ...
Yueyuan Yin +6 more
openaire +2 more sources
Structure of a VHH isolated from a naïve phage display library
BMC Research Notes, 2019 Objective To determine the X-ray structure and biophysical properties of a Camelid VHH isolated from a naïve phage display library. Results Single domain antibodies (VHH) derived from the unique immune system of the Camelidae family have gained traction ...
Brandy White, Ian Huh, Cory L. Brooks
doaj +1 more source
Molecular Therapy: Nucleic Acids, 2020 To date, mRNA-based biologics have mainly been developed for prophylactic and therapeutic vaccination to combat infectious diseases or cancer. In the past years, optimization of the characteristics of in vitro transcribed mRNA has led to significant ...
Lien Van Hoecke +10 more
doaj +1 more source
Single-domain antibodies for biomedical applications [PDF]
Immunopharmacology and Immunotoxicology, 2015 Single-domain antibodies are the smallest antigen-binding units of antibodies, consisting either only of one variable domain or one engineered constant domain that solely facilitates target binding. This class of antibody derivatives comprises naturally occurring variable domains derived from camelids and sharks as well as engineered human variable or ...
Krah, Simon +5 more
openaire +3 more sources
Bglbrick strategy for the construction of single domain antibody fusions
Heliyon, 2017 Single domain antibodies, recombinantly expressed variable domains derived from camelid heavy chain antibodies, are often expressed as multimers for detection and therapeutic applications.
Ellen R. Goldman +3 more
doaj +1 more source
Photobodies: Light‐Activatable Single‐Domain Antibody Fragments [PDF]
Angewandte Chemie, 2019 AbstractPhotocaged antibody fragments, termed photobodies, have been developed that are impaired in their antigen‐binding capacity and can be activated by irradiation with UV light (365 nm). This rational design concept builds on the selective photocaging of a single tyrosine in a nanobody (a single‐domain antibody fragment).
Benedikt Jedlitzke +3 more
openaire +3 more sources
NbX: Machine Learning-Guided Re-Ranking of Nanobody–Antigen Binding Poses
Pharmaceuticals, 2021 Modeling the binding pose of an antibody is a prerequisite to structure-based affinity maturation and design. Without knowing a reliable binding pose, the subsequent structural simulation is largely futile.
Chunlai Tam +2 more
doaj +1 more source
Plant-derived single domain COVID-19 antibodies
Journal of Controlled Release, 2023 Data show a decrease in the risk of hospitalization and death from COVID-19. To date, global vaccinations for SARS-CoV-2 protections are underway, but additional treatments are urgently needed to prevent and cure infection among naïve and even vaccinated people.
Shen, Andrew M. +3 more
openaire +2 more sources