Results 21 to 30 of about 763,272 (322)
Single-domain antibody screening by isPLA-seq [PDF]
Life Science Alliance, 2021 Single-domain antibody (sdAb) holds the promising strategies for diverse research and translational applications. Here, we describe a method for the adaptation of the in situ proximity ligation assay (isPLA) followed by sequencing (isPLA-seq) to facilitate screening of a high-sensitive, high-throughput sdAb library for a given protein at subcellular ...
Yueyuan Yin +6 more
openaire +2 more sources
A Human-Derived Monoclonal Antibody Targeting Extracellular Connexin Domain Selectively Modulates Hemichannel Function [PDF]
, 2019 Connexin hemichannels, which are plasma membrane hexameric channels (connexons) composed of connexin protein protomers, have been implicated in a host of physiological processes and pathological conditions. A number of single point pathological mutations
Buratto, Damiano +12 more
core +1 more source
Bglbrick strategy for the construction of single domain antibody fusions
Heliyon, 2017 Single domain antibodies, recombinantly expressed variable domains derived from camelid heavy chain antibodies, are often expressed as multimers for detection and therapeutic applications.
Ellen R. Goldman +3 more
doaj +1 more source
Molecular Therapy: Nucleic Acids, 2020 To date, mRNA-based biologics have mainly been developed for prophylactic and therapeutic vaccination to combat infectious diseases or cancer. In the past years, optimization of the characteristics of in vitro transcribed mRNA has led to significant ...
Lien Van Hoecke +10 more
doaj +1 more source
Structure of a VHH isolated from a naïve phage display library
BMC Research Notes, 2019 Objective To determine the X-ray structure and biophysical properties of a Camelid VHH isolated from a naïve phage display library. Results Single domain antibodies (VHH) derived from the unique immune system of the Camelidae family have gained traction ...
Brandy White, Ian Huh, Cory L. Brooks
doaj +1 more source
Photobodies: Light‐Activatable Single‐Domain Antibody Fragments [PDF]
Angewandte Chemie, 2019 AbstractPhotocaged antibody fragments, termed photobodies, have been developed that are impaired in their antigen‐binding capacity and can be activated by irradiation with UV light (365 nm). This rational design concept builds on the selective photocaging of a single tyrosine in a nanobody (a single‐domain antibody fragment).
Benedikt Jedlitzke +3 more
openaire +3 more sources
NbX: Machine Learning-Guided Re-Ranking of Nanobody–Antigen Binding Poses
Pharmaceuticals, 2021 Modeling the binding pose of an antibody is a prerequisite to structure-based affinity maturation and design. Without knowing a reliable binding pose, the subsequent structural simulation is largely futile.
Chunlai Tam +2 more
doaj +1 more source
Conjugation of biotin-coated luminescent quantum dots with single domain antibody-rhizavidin fusions
Biotechnology Reports, 2016 Straightforward and effective methods are required for the bioconjugation of proteins to surfaces and particles. Previously we demonstrated that the fusion of a single domain antibody with the biotin binding molecule rhizavidin provided a facile method ...
Jinny L. Liu +9 more
doaj +1 more source
Single-domain antibodies and their formatting to combat viral infections [PDF]
, 2018 Since their discovery in the 1990s, single-domain antibodies (VHHs), also known as NanobodiesA (R), have changed the landscape of affinity reagents. The outstanding solubility, stability, and specificity of VHHs, as well as their small size, ease of ...
Ballegeer, Marlies +4 more
core +2 more sources
Plant-derived single domain COVID-19 antibodies
Journal of Controlled Release, 2023 Data show a decrease in the risk of hospitalization and death from COVID-19. To date, global vaccinations for SARS-CoV-2 protections are underway, but additional treatments are urgently needed to prevent and cure infection among naïve and even vaccinated people.
Shen, Andrew M. +3 more
openaire +2 more sources