Results 41 to 50 of about 737,167 (335)

Single domain antibodies targeting neuraminidase protect against an H5N1 influenza virus challenge [PDF]

, 2014
Influenza virus neuraminidase (NA) is an interesting target of small-molecule antiviral drugs. We isolated a set of H5N1 NAspecific single-domain antibodies (N1-VHHm) and evaluated their in vitro and in vivo antiviral potential. Two of them inhibited the
Cardoso, Francisco Miguel, De Baets, Sarah, Depicker, Ann, Descamps, Francis J., Fiers, Walter, Ibañez, Lorena Itatí, Muyldermans, Serge, Roose, Kenny, Saelens, Xavier, Schepens, Bert, Smet, Anouk, Van Den Hoecke, Silvie   +11 more
core   +2 more sources

Humanized single domain antibodies neutralize SARS-CoV-2 by targeting the spike receptor binding domain

Nature Communications, 2020
Here, using a humanized phage display library with recombinant SARS- CoV-2 receptor binding domain (RBD) proteins, the authors identify a number of single domain antibodies (sdAbs) that neutralize SARS-CoV-2 in vitro by inhibiting the interaction of the ...
Xiaojing Chi, Xiuying Liu, Conghui Wang, Xinhui Zhang, Xiang Li, Jianhua Hou, Lili Ren, Qi Jin, Jianwei Wang, Wei Yang   +9 more
doaj   +1 more source

Arginine mutation alters binding of a human monoclonal antibody to antigens linked to systemic lupus erythematosus and the antiphospholipid syndrome [PDF]

, 2007
Objective: Previous studies have shown the importance of somatic mutations and arginine residues in the complementarity-determining regions (CDRs) of pathogenic anti-double-stranded DNA (anti-dsDNA) antibodies in human and murine lupus, and in studies of
Giles, I., Ioannou, Y., Isenberg, D.A., Lambrianides, A., Latchman, D.S., Manson, J.J., Mason, L., Rahman, A.   +7 more
core   +1 more source

A Human-Derived Monoclonal Antibody Targeting Extracellular Connexin Domain Selectively Modulates Hemichannel Function [PDF]

, 2019
Connexin hemichannels, which are plasma membrane hexameric channels (connexons) composed of connexin protein protomers, have been implicated in a host of physiological processes and pathological conditions. A number of single point pathological mutations
Buratto, Damiano, Carrer, Andrea, Chiani, Francesco, Kuang, Yuanyuan, Lerner, Richard A, Mammano, Fabio, Nardin, Chiara, Paludetti, Gaetano, Salvatore, Anna Maria, Xu, Liang, Yang, Guang, Ziraldo, Gaia, Zonta, Francesco   +12 more
core   +1 more source

Single‐Domain Antibodies and Their Utility

Current Protocols in Immunology, 2013
AbstractEngineered monoclonal antibody fragments have gained market attention due to their versatility and tailor‐made potential and are now considered to be an important part of future immunobiotherapeutics. Single‐domain antibodies (sdAbs), also known as nanobodies, are derived from VHHs [variable domains (V) of heavy‐chain‐only antibodies (HCAb)] of
Baral, Toya Nath, MacKenzie, Roger, Ghahroudi, Mehdi Arbabi   +2 more
openaire   +4 more sources

Requirement of the FATC domain of protein kinase Tel1 for localization to DNA ends and target protein recognition [PDF]

, 2015
Two large phosphatidylinositol 3-kinase-related protein kinases (PIKKs), ATM and ATR, play a central role in the DNA damage response pathway. PIKKs contain a highly conserved extreme C-terminus called the FRAP-ATM-TRRAP-C-terminal (FATC) domain.
Ghosh, A., Goto, G.H., Henry, E., Ogi, H., Sugimoto, K., Zencir, Sevil   +5 more
core   +1 more source

Delicate balance among thermal stability, binding affinity, and conformational space explored by single-domain VHH antibodies

Scientific Reports, 2021
The high binding affinities and specificities of antibodies have led to their use as drugs and biosensors. Single-domain VHH antibodies exhibit high specificity and affinity but have higher stability and solubility than conventional antibodies as they ...
Emina Ikeuchi, Daisuke Kuroda, Makoto Nakakido, Akikazu Murakami, Kouhei Tsumoto   +4 more
doaj   +1 more source

Identification of an N-terminal glycogen synthase kinase 3 phosphorylation site which regulates the functional localisation of polycystin-2 in vivo and in vitro [PDF]

, 2006
PKD2 is mutated in 15% of patients with autosomal dominant polycystic kidney disease (ADPKD). Polycystin-2 (PC2), the PKD2 protein, is a nonselective Ca2 + -permeable cation channel which may function at the cell surface and ER. Nevertheless, the factors
Kane, M.E., Moon, D.J., Obara, T., Ong, A.C.M., Streets, A.J.   +4 more
core   +2 more sources

The replacement histone H2A.Z in a hyperacetylated form is a feature of active genes in the chicken [PDF]

, 2005
The replacement histone H2A.Z is variously reported as being linked to gene expression and preventing the spread of heterochromatin in yeast, or concentrated at heterochromatin in mammals.
Bonifer, C., Bruce, K., Crane-Robinson, C., Greaves, I., Lefevre, P., Mantouvalou, E., Myers, F.A., Thorne, A.W., Tremethick, D.J.   +8 more
core   +5 more sources

Enhancing neutralizing activity against influenza H1N1/PR8 by engineering a single-domain VL-M2 specific into a bivalent form.

PLoS ONE, 2022
Flu disease, with high mortality and morbidity, is caused by the influenza virus. Influenza infections are most effectively prevented through vaccination, but it requires annual reformulation due to the antigenic shift or drift of hemagglutinin and ...
Phuong Thi Hoang, Quynh Xuan Thi Luong, Seungchan Cho, Yongjun Lee, Kyungho Na, Ramadhani Qurrota Ayun, Thuy Thi Bich Vo, Taehyun Kim, Sukchan Lee   +8 more
doaj   +1 more source