Results 141 to 150 of about 13,695 (259)

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Epigenetic and Transcriptomic Impacts of Ethanol Vary by Brain Region and Extent of Exposure. [PDF]

eNeuro
Periandri EM, Dodson KM, Lopes M, de Luna Vitorino FN, Ola A, Gongora JM, Garcia BA, Glastad KM, Egervari G.   +8 more
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

A multifaceted antimicrobial stewardship intervention to improve antibiotic utilization and prescribing for sinusitis in a large primary care network. [PDF]

Antimicrob Steward Healthc Epidemiol
Sportiello M, Samoriski C, Kim C, Pomakov A, Anlage T, Dahal A, Delacruz L, Gigas J, Kim J, Sudnik P, Livada A, Masterson M, Springer C, Bennett J, Lopa S, Venci J, Fortuna RJ, Yamshchikov A.   +17 more
europepmc   +1 more source

Long‐term hippocampal alterations and cognitive impairment in a murine model of surgical sepsis

FEBS Open Bio, EarlyView.
Using a mouse model of surgical sepsis, we tested long‐term memory and analyzed the transcriptome of single cells isolated from the hippocampus. Survivor mice showed worse memory, loss of certain brain cell subpopulations, and abnormal immune cell activity—suggesting that post‐sepsis brain alterations may be linked to cognitive deficits.
Dong Seong Cho, Rebecca Schmitt, Aneesha Dasgupta, Alexandra Ducharme, Jason Doles   +4 more
wiley   +1 more source

Epidemiology of microbiological findings in the lower respiratory tract in mechanically ventilated patients with and without inhalation injury. [PDF]

Front Cell Infect Microbiol
Polackova T, Vyklicka K, Lipovy B, Bohm J, Raska F, Tresnerova I, Hladik M, Kocmanova I, Hanslianova M, Borilova Linhartova P.   +9 more
europepmc   +1 more source

Identifying transcription factors controlling the basal expression of human MRP4 highlights a substantial role for Sp1

FEBS Open Bio, EarlyView.
The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer, Angelique Kragl, Katrin Ziems, Juliane Glaubitz, Sophie Grammbauer, Susanne Hildebrandt, Mladen V. Tzvetkov, Gabriele Jedlitschky   +7 more
wiley   +1 more source

The role of emotions, stress, learning stage, and cognitive load on learning in emergency events: a mixed methods study. [PDF]

Sci Rep
Alismail A, Lopez D, Vosko A, Ireland K, Tan LD.   +4 more
europepmc   +1 more source

Molecular dynamics simulations of positively selected codons in FcγRI reveal novel biochemical binding properties

FEBS Open Bio, EarlyView.
Evolutionary analysis across 32 placental mammals identified positive selection at residues H148 and W149 in the immune receptor FcγR1. Ancestral reconstruction combined with molecular dynamics simulations reveals how these mutations may influence receptor structure and dynamics, providing insight into the evolution of antibody recognition and immune ...
David A. Young, Omar Arias‐Gaguancela, Fiona Gibson, Morgan Grainger, Jodie Score, Amit Nathubhai, Mauricio Cafiero, Lee Richard Machado   +7 more
wiley   +1 more source

Real-World Prevalence and Structural Validation of the Canonical 9p21 MTAP-CDKN2A/B Deletion in Non-NSCLC Solid Tumors. [PDF]

Cancers (Basel)
Han M, Lee E, Shin JE, Kwon M, Hong JY, Kim ST, Ahn S, Kim KM, Lee J, Lim SH.   +9 more
europepmc   +1 more source