Results 11 to 20 of about 354,238 (282)

Site-Directed Mutagenesis of Large Plasmids

BioTechniques, 1998
A protocol combining recombination PCR and long-distance PCR is demonstrated to be highly accurate and rapid for sitedirected mutagenesis of large (>10 kb) plasmids.
Susan A. Nadin-Davis, Shirley C. Chang
doaj   +1 more source

Combining rational and random strategies in β-glucosidase Zm-p60.1 protein library construction. [PDF]

PLoS ONE, 2014
Saturation mutagenesis is a cornerstone technique in protein engineering because of its utility (in conjunction with appropriate analytical techniques) for assessing effects of varying residues at selected positions on proteins' structures and functions.
Dušan Turek, Pavel Klimeš, Pavel Mazura, Břetislav Brzobohatý   +3 more
doaj   +1 more source

REMP software to introduce a screening REstriction site in site-directed Mutagenesis Primer

SoftwareX, 2021
Insertion of silent mutations allowing for restriction site modification aids in the screening of successful mutants during site directed mutagenesis. Introducing a new restriction site requires the analysis of degenerate sequences within mutant primer ...
Madhavi Latha Yadav Bangaru, PhD, Ravi Kumar Medabalimi, Sobhan Babu, PhD, Nidhanapati K. Raghavendra, PhD   +3 more
doaj   +1 more source

Long-range electron transfer in structurally engineered pentaammineruthenium (histidine-62) cytochrome c [PDF]

, 1989
In many biological processes, long-range electron transfer (ET) plays a key role. When the three-dimensional structures of proteins are accurately known, use of modified proteins and protein-protein complexes provides an experimental approach to study ET
Bowler, Bruce E., Gray, Harry B., Mayo, Stephen L., Meade, Thomas J., Richards, John H.   +4 more
core   +1 more source

Protein engineering of Pseudomonas fluorescens peroxidase Dyp1B for oxidation of phenolic and polymeric lignin substrates [PDF]

, 2019
Directed evolution was applied to dye-decolourizing peroxidase Dyp1B from Pseudomonas fluorescens Pf-5, in order to enhance the activity for oxidation of phenolic and lignin substrates.
Ashley, Ben, Bugg, Timothy D H, Ehibhatiomhan, Austine, Huang, Yuling, Mendel Williams, Sharon, Pour, Rahman Rahman, Rashid, Goran   +6 more
core   +2 more sources

Role of tyrosine M210 in the initial charge separation of reaction centers of Rhodobacter sphaeroides [PDF]

, 1990
Femtosecond spectroscopy was used in combination with site-directed mutagenesis to study the influence of tyrosine M210 (YM210) on the primary electron transfer in the reaction center of Rhodobacter sphaeroides.
Allen J. P., Becker M., Breton J., Bylina E. J., Bylina E. J., Chang C. H., Christoph Lauterwasser, Deisenhofer J., Dieter Oesterhelt, Farchaus J., Feher G., Gray K. A., Holzapfel W., Holzapfel W., Kevin A. Gray, Kirmaier C., Kirmaier C., Kirmaier C., Okamura Y., Parson W., Parson W. W., Rees D. C., Stilz H. U., Tiede D. M., Ulrich Finkele, Wolfgang Zinth   +25 more
core   +1 more source

SUMOylation of nuclear actin [PDF]

, 2009
Actin, a major component of the cytoplasm, is also abundant in the nucleus. Nuclear actin is involved in a variety of nuclear processes including transcription, chromatin remodeling, and intranuclear transport.
Alessandro Arduini, Aslan, Ball, Camacho, Camacho, Carlos J. Camacho, Chik, Chuang, Comeau, Comeau, Dantan-Gonzalez, Dignam, dos Remedios, Dundr, Field, Frances V. Fuller-Pace, Hay, Hofmann, Hofmann, Jacobs, James L. Lessard, Jockusch, Johnson, Kalderon, Karakozova, Kozakov, Kudo, Kudryashova, Lin, Lorenz, Louvet, Mandell, Matic, Matsuzaki, McDonald, Panse, Pendleton, Pestic-Dragovich, Posern, Primal de Lanerolle, Reverter, Rodriguez, Rosas-Acosta, Samantha M. Nicol, Schoenenberger, Schoenenberger, Stuven, Thom, Vertegaal, Wada, Wang, Westphal, Wilma A. Hofmann   +52 more
core   +5 more sources

A new approach to 'megaprimer' polymerase chain reaction mutagenesis without an intermediate gel purification step

BMC Biotechnology, 2004
Background Site-directed mutagenesis is an efficient method to alter the structure and function of genes. Here we report a rapid and efficient megaprimer-based polymerase chain reaction (PCR) mutagenesis strategy that by-passes any intermediate ...
Duggleby Ronald G, Lai Richard, Tyagi Rajiv   +2 more
doaj   +1 more source

Engineering of Cyclodextrin Product Specificity and pH Optima of the Thermostable Cyclodextrin Glycosyltransferase from Thermoanaerobacterium thermosulfurigenes EM1 [PDF]

, 1998
The product specificity and pH optimum of the thermostable cyclodextrin glycosyltransferase (CGTase) from Thermoanaerobacterium thermosulfurigenes EM1 was engineered using a combination of x-ray crystallography and site-directed mutagenesis.
Bauke W. Dijkstra, Bhat, Bradford, Brünger, Davies, Engh, Fujiwara, Haeckel, Harata, Jones, Joost C.M. Uitdehaag, Kabsch, Klein, Klein, Kleywegt, Knegtel, Knegtel, Koshland, Lamzin, Laskowski, Lawson, Lazzaroni, Lubbert Dijkhuizen, Monma, Nakamura, Nakamura, Norman, Pedersen, Penninga, Qian, Read, Reinetta M. Buitelaar, Richèle D. Wind, Sambrook, Sanger, Schmid, Starnes, Strokopytov, Strokopytov, Svensson, Takase, Takusagawa, Tronrud, Wind, Yanisch-Perron   +44 more
core   +2 more sources

High-Potential C112D/M121X (X = M, E, H, L) Pseudomonas aeruginosa Azurins [PDF]

, 2009
Site-directed mutagenesis of Pseudomonas aeruginosa azurin C112D at the M121 position has afforded a series of proteins with elevated Cu^(II/I) reduction potentials relative to the CuII aquo ion.
Gray, Harry B., Lancaster, Kyle M., Richards, John H., Winkler, Jay R., Yokoyama, Keiko   +4 more
core   +3 more sources