Results 61 to 70 of about 354,238 (282)

Conserved Aspartate Residues and Phosphorylation in Signal Transduction by the Chemotaxis Protein CheY [PDF]

, 1990
The CheY protein is phosphorylated by CheA and dephosphorylated by CheZ as part of the chemotactic signal transduction pathway in Escherichia coli. Phosphorylation of CheY has been proposed to occur on an aspartate residue.
Bourret, Robert B., Hess, J. Fred, Simon, Melvin I.   +2 more
core   +2 more sources

Structural biology of ferritin nanocages

FEBS Letters, EarlyView.
Ferritin is a conserved iron‐storage protein that sequesters iron as a ferric mineral core within a nanocage, protecting cells from oxidative damage and maintaining iron homeostasis. This review discusses ferritin biology, structure, and function, and highlights recent cryo‐EM studies revealing mechanisms of ferritinophagy, cellular iron uptake, and ...
Eloise Mastrangelo, Flavio Di Pisa
wiley   +1 more source

Identification of a Key Amino Acid of the Human 5-HT2B Serotonin Receptor Important for Sarpogrelate Binding

Journal of Pharmacological Sciences, 2007
Based on radio-ligand binding and molecular modeling studies, sarpogrelate shows a moderate selectivity for 5-HT2B versus 5-HT2A receptors. To confirm the modeling data of sarpogrelate to 5-HT2B receptors predicting interaction of sarpogrelate towards ...
Habib Abul Muntasir, Murad Hossain, Mohiuddin Ahmed Bhuiyan, Tadazumi Komiyama, Takashi Nakamura, Masanobu Ozaki, Takafumi Nagatomo   +6 more
doaj   +1 more source

High-throughput Site-directed Scanning Mutagenesis Using a Two-fragment PCR Approach

Bio-Protocol, 2020
Site-directed scanning mutagenesis is a useful tool applied in studying protein function and designing proteins with new properties, such as increased stability or enzymatic activity. Creating a systematic library of hundreds of site-directed mutants is
Franziska Heydenreich, Tamara Miljuš, Dalibor Milić, Dmitry Veprintsev   +3 more
doaj   +1 more source

Efficient method for site-directed mutagenesis in large plasmids without subcloning. [PDF]

PLoS ONE, 2017
Commonly used methods for site-directed DNA mutagenesis require copying the entire target plasmid. These methods allow relatively easy modification of DNA sequences in small plasmids but become less efficient and faithful for large plasmids ...
Louay K Hallak, Kelly Berger, Rita Kaspar, Anna R Kwilas, Federica Montanaro, Mark E Peeples   +5 more
doaj   +1 more source

YAP1::TFE3 mediates endothelial‐to‐mesenchymal plasticity in epithelioid hemangioendothelioma

Molecular Oncology, EarlyView.
The YAP1::TFE3 fusion protein drives endothelial‐to‐mesenchymal transition (EndMT) plasticity, resulting in the loss of endothelial characteristics and gain of mesenchymal‐like properties, including resistance to anoikis, increased migratory capacity, and loss of contact growth inhibition in endothelial cells.
Ant Murphy, Samuel Hartzler, Paula A. Vargas Carranza, Shyaman Jayasundara, Madison E. Yates, Nimod D. Janson, Bozhi Liu, Annaleigh Benton, Majid Kazemian, Jason A. Hanna   +9 more
wiley   +1 more source

Human carboxylesterase 2: Studies on the role of glycosylation for enzymatic activity

Biochemistry and Biophysics Reports, 2016
Human carboxylesterase 2 (hCES2) is a glycoprotein involved in the metabolism of drugs and several environmental xenobiotics, whose crystallization has been proved to be a challenging task.
Márcia Alves, Joana Lamego, Tiago Bandeiras, Rute Castro, Hélio Tomás, Ana Sofia Coroadinha, Júlia Costa, Ana Luisa Simplício   +7 more
doaj   +1 more source

Structure and mechanism of acetolactate decarboxylase [PDF]

, 2013
Acetolactate decarboxylase catalyzes the conversion of both enantiomers of acetolactate to the (R)-enantiomer of acetoin, via a mechanism that has been shown to involve a prior rearrangement of the non-natural (R)-enantiomer substrate to the natural (S ...
Anand R., Chan K. K., Collaborative Computational Project Number 4, Crout D. H. G., Crout D. H. G., Crout D. H. G., Crout D. H. G., Crout D. H. G., Dean Rea, DeLano W. L., Dolin M. I., Drake A. F., Dulieu C., Fisher Z., Fusetti F., Gerlt J. A., Hall D. R., Hill R. K., Ho M.-C., Jones T. A., Kabsch W., Løken J. P, Martin Wills, Moen A. R., Murshudov G. N., Najmudin S., Okrasa K., Perrakis A., Read R. J., Robins D. J., Shabir Najmudin, Stritzke K., Størmer F. C., Victoria A. Marlow, Vilmos Fülöp, Vinogradov M., Yan Y.   +36 more
core   +1 more source

Class IIa HDACs forced degradation allows resensitization of oxaliplatin‐resistant FBXW7‐mutated colorectal cancer

Molecular Oncology, EarlyView.
HDAC4 is degraded by the E3 ligase FBXW7. In colorectal cancer, FBXW7 mutations prevent HDAC4 degradation, leading to oxaliplatin resistance. Forced degradation of HDAC4 using a PROTAC compound restores drug sensitivity by resetting the super‐enhancer landscape, reprogramming the epigenetic state of FBXW7‐mutated cells to resemble oxaliplatin ...
Vanessa Tolotto, Nicolò Gualandi, Ylenia Cortolezzis, Raffaella Picco, Monica Colitti, Francesca D'Este, Mariachiara Gani, Wayne W. Hancock, Giovanni Terrosu, Cristina Degrassi, Francesca Agostini, Claudio Brancolini, Luigi E. Xodo, Eros Di Giorgio   +13 more
wiley   +1 more source

Cell surface interactome analysis identifies TSPAN4 as a negative regulator of PD‐L1 in melanoma

Molecular Oncology, EarlyView.
Using cell surface proximity biotinylation, we identified tetraspanin TSPAN4 within the PD‐L1 interactome of melanoma cells. TSPAN4 negatively regulates PD‐L1 expression and lateral mobility by limiting its interaction with CMTM6 and promoting PD‐L1 degradation.
Guus A. Franken, Andrea Abel Gutierrez, Imke van Rossum, Cornelia G. Spruijt, Michiel Vermeulen, Guido van Mierlo, Blanca Scheijen, Annemiek B. van Spriel   +7 more
wiley   +1 more source