Identification of critical residues of the serotype modifying O-acetyltransferase of Shigella flexneri [PDF]
, 2016 BACKGROUND Thirteen serotypes of Shigella flexneri (S. flexneri) have been recognised, all of which are capable of causing bacillary dysentery or shigellosis. With the emergence of the newer S.
Thanweer, Farzaana, Verma, Naresh K
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Identification of a key residue for Oligomerisation and pore-formation of Clostridium perfringens NetB [PDF]
, 2014 Necrotic enteritis toxin B (NetB) is a β-pore-forming toxin produced by Clostridium perfringens and has been identified as a key virulence factor in the pathogenesis of avian necrotic enteritis, a disease causing significant economic damage to the ...
Basak, Ajit K. +6 more
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Creating Randomized Amino Acid Libraries with the QuikChange® Multi Site-Directed Mutagenesis Kit
BioTechniques, 2002 The QuikChange® Multi Site-Directed Mutagenesis Kit is a simple and efficient method for introducing point mutations at up to five sites simultaneously in plasmid DNA templates. Here we used the QuikChange Multi kit with degenerate (one codon) primers to
Holly H. Hogrefe +3 more
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Probing the Electrostatic and Steric Requirements for Substrate Binding in Human Platelet-Type 12-Lipoxygenase. [PDF]
, 2019 Human platelet ALOX12 (hALOX12 or h12-LOX) has been implicated in a variety of human diseases. The present study investigates the active site of hALOX12 to more thoroughly understand how it positions the substrate and achieves nearly perfect regio- and ...
Aleem, Ansari Mukhtar +7 more
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Engineering Dehydrated Amino Acid Residues in the Antimicrobial Peptide Nisin [PDF]
, 1992 The small antimicrobial peptide nisin, produced by Lactococcus lactis, contains the uncommon amino acid residues dehydroalanine and dehydrobutyrine and five thio ether bridges.
Boot, Hein J., +5 more
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eLife, 2015 Enzymes enable life by accelerating reaction rates to biological timescales. Conventional studies have focused on identifying the residues that have a direct involvement in an enzymatic reaction, but these so-called ‘catalytic residues’ are embedded in ...
Fanny Sunden +4 more
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Site directed mutagenesis and purification of the cDNA for human class I aldehyde dehydrogenase : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science at Massey University [PDF]
, 1998 Aldehyde dehydrogenase (ALDH) is a key enzyme of alcohol metabolism, removing acetaldehyde which is formed as a product of the alcohol dehydrogenase reaction.
Wansbrough, Erin M
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Catalysts on Demand: Selective Oxidations by Laboratory-Evolved Cytochrome P450 BM3 [PDF]
, 2009 Efficient catalysts for selective oxidation of C-H bonds using atmospheric oxygen are highly desirable to decrease the economic and environmental costs associated with conventional oxidation processes.
Arnold, Frances H., Lewis, Jared C.
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Biotechnology & Biotechnological Equipment, 2017 Introducing various mutations at multiple specific sites within a gene requires multiple steps of DNA manipulation, which is the initial, but limiting step of protein structure–function studies.
Fanli Zeng +4 more
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One-Step, Highly Efficient Site-Directed Mutagenesis by Toxic Protein Selection
BioTechniques, 2002 A fast and efficient site-directed mutagenesis method has been developed, using the newly constructed plasmid pTPS19, which expresses the toxic CcdB protein originally encoded by the E. coli F plasmid.
W. Xu +7 more
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