Results 61 to 70 of about 593 (218)

The intracellular localization of malate dehydrogenase isoenzymes in Pisum arvense roots

Acta Societatis Botanicorum Poloniae, 2014
Mitochondria and plastids were isolated from Pisum arvense root cells by sucrose density gradient centrifugation. The individual subcellular fractions so obtained were subjected to isoelectric focusing on cellulose acetate strips.
Genowefa Kubik-Dorosz
doaj   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Dynamics of water status during fruiting body development of Agaricus bisporus and its relationship to mushroom quality

Chemical and Biological Technologies in Agriculture
Background The analysis of changes in subcellular water distribution during mushroom fruiting is essential for elucidating the movement of water molecules within subcellular compartments.
Lingzhong Wan, Hui Sun, Ying Ni, Jun Tang, Junjie Yu, Juan Wu, Xiaoming Yan, Xiaonan Deng   +7 more
doaj   +1 more source

Sub-cellular Proteomics of Medicago truncatula

Frontiers in Plant Science, 2013
Medicago truncatula is a leading model species and substantial molecular, genetic, genomics, proteomics and metabolomics resources have been developed for this species to facilitate the study of legume biology. Currently, over 60 proteomics studies of M.
Jeonghoon eLee, Zhentian eLei, Bonnie eWatson, Lloyd W. Sumner   +3 more
doaj   +1 more source

Aquaporin‐3 and aquaporin‐5 impact the development of pancreatic ductal adenocarcinoma spheroids

FEBS Open Bio, EarlyView.
Schematic representation of the role of aquaporin‐3 (AQP3) and aquaporin‐5 (AQP5) in pancreatic ductal adenocarcinoma (PDAC). Both proteins are upregulated in PDAC and are associated with tumor progression and metastatic potential. Silencing AQP3 or AQP5 in PDAC spheroids results in decreased diameter, area, and overall growth, underscoring their key ...
Catarina Pimpão, Diogo M. Engrácia, Graça Soveral, Filipa Mendes   +3 more
wiley   +1 more source

Hyperactive ice‐binding proteins stabilize cell membranes and improve resistance to dehydration stress in Caenorhabditis elegans

FEBS Open Bio, EarlyView.
TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose, Kotaro Ozaki, Ryohei Kuriyama, Yuka Ikemoto, Kazuhiro Mio, Yuji C. Sasaki, Tatsuya Arai, Sakae Tsuda, Yoichi Shinkai, Masahiro Kuramochi   +9 more
wiley   +1 more source

Protection of Chicken against Bacterial Infection by Immunization with Bacterial Subcellular Fractions

Al-Mustansiriyah Journal of Pharmaceutical Sciences, 2007
The current studies were undertaken to assess the ability of various subcellular fraction of E.coli to induce protection against challenge with E.coli in chicken.
Khalid K. AL.Bayatti, Samia K. Mahmood, Ali A. Abdul-Abbas   +2 more
doaj   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

FEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas, Aaron D. Morgan, Ignas Čiurlionis, Christian Böhm, Stefan Jockenhövel, Willi Jahnen‐Dechent, Marc A. M. J. van Zandvoort   +6 more
wiley   +1 more source

Behavior of sterols of insect muscle during homogenization and differential centrifugation

Journal of Lipid Research, 1968
Labeled sterol introduced in vitro into a homogenate of insect muscle became distributed in the various subcellular fractions in a manner similar to that obtained by fractionation of muscle of insects to which labeled sterol had been administered in vivo.
W.R. Roeske, R.B. Clayton
doaj   +1 more source