Results 81 to 90 of about 95,299 (308)

Phospholipids and acyl groups of subcellular membrane fractions from human intracranial tumors

Journal of Lipid Research, 1974
The phospholipids from subcellular fractions of human intracranial tumors were examined. For comparison, microsomes were isolated from a fetal human brain and from the gray matter of adult human brains.
G.Y. Sun, B.S. Leung
doaj   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

The intracellular localization of malate dehydrogenase isoenzymes in Pisum arvense roots

Acta Societatis Botanicorum Poloniae, 2014
Mitochondria and plastids were isolated from Pisum arvense root cells by sucrose density gradient centrifugation. The individual subcellular fractions so obtained were subjected to isoelectric focusing on cellulose acetate strips.
Genowefa Kubik-Dorosz
doaj   +1 more source

A Yeast Chromatin-enriched Fractions Purification Approach, yChEFs, from Saccharomyces cerevisiae

Bio-Protocol, 2020
We have adapted a previous procedure and improved an approach that we named yChEFs (yeast Chromatin Enriched Fractions) for purifying chromatin fractions.
Abel Cuevas-Bermúdez, Ana Garrido-Godino, Francisco Gutiérrez-Santiago, Francisco Navarro   +3 more
doaj   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

FEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas, Aaron D. Morgan, Ignas Čiurlionis, Christian Böhm, Stefan Jockenhövel, Willi Jahnen‐Dechent, Marc A. M. J. van Zandvoort   +6 more
wiley   +1 more source

Dynamics of water status during fruiting body development of Agaricus bisporus and its relationship to mushroom quality

Chemical and Biological Technologies in Agriculture
Background The analysis of changes in subcellular water distribution during mushroom fruiting is essential for elucidating the movement of water molecules within subcellular compartments.
Lingzhong Wan, Hui Sun, Ying Ni, Jun Tang, Junjie Yu, Juan Wu, Xiaoming Yan, Xiaonan Deng   +7 more
doaj   +1 more source

Sub-cellular Proteomics of Medicago truncatula

Frontiers in Plant Science, 2013
Medicago truncatula is a leading model species and substantial molecular, genetic, genomics, proteomics and metabolomics resources have been developed for this species to facilitate the study of legume biology. Currently, over 60 proteomics studies of M.
Jeonghoon eLee, Zhentian eLei, Bonnie eWatson, Lloyd W. Sumner   +3 more
doaj   +1 more source

Transformations of Progesterone by Subcellular Fractions of Human Skin

, 1975
Homogenates of human skin metabolize progesterone-4-14C to the same products as whole skin. Studies with subcellular fractions obtained by differential centrifugation indicate the presence in skin of a membrane-bound 5α-reductase and a soluble 20α ...
Gomez, E C, Frost, P., Hsia, S L
core   +1 more source

Malformin A1–mediated cytotoxicity in ovarian cancer cells occurs through pyroptosis and autophagy

FEBS Open Bio, EarlyView.
This study investigated the effects of the natural compound Malformin A1 (MA1) on the cytoskeleton that regulates cell proliferation and migration. Disruption of the cytoskeleton can impair these processes and promote cancer cell death. MA1 disrupted cytoskeletal organization, induced DNA damage, inflammation, activated autophagy, and pyroptosis ...
Nada Abdullah Hassan, Ikram A. Burney, Shika Hanif Malgundkar, Siva Ramamoorthy, Ressin Varghese, Mohamed A. Al‐Kindi, Hamad Al Riyami, Shadia Al Bahlani, Sergey Dobretsov, Syed Imran Hassan, Benjamin K. Tsang, Yahya Tamimi   +11 more
wiley   +1 more source

Mutant NPM1 in Acute Myeloid Leukemia Initiation and Maintenance

Aging and Cancer, EarlyView.
NPM1 mutations drive acute myeloid leukemia by acting as neomorphic transcriptional regulators that cooperate with Menin–MLL and XPO1 to sustain HOX/MEIS1 expression and block differentiation. Targeting these mutant‐specific transcriptional dependencies provides a rational therapeutic strategy for NPM1‐mutated AML.
Yanan Jiang, Zhong Fan, Runmeng Liu, Xiaotian Zhang   +3 more
wiley   +1 more source