Simultaneous Quantification of the Acetylome and Succinylome by One-Pot Affinity Enrichment. [PDF]
PROTEOMICS, Volume 18, Issue 17, September 2018., 2018 Protein posttranslational modifications (PTMs) are of increasing interest in biomedical research, yet studies rarely examine more than one PTM. One barrier to multi-PTM studies is the time cost for both sample preparation and data acquisition, which ...
Basisty, Nathan +4 more
core +7 more sources
Quantitative Analysis of the Proteome and the Succinylome in the Thyroid Tissue of High-Fat Diet-Induced Hypothyroxinemia in Rats [PDF]
International Journal of Endocrinology, 2020 Hypothyroidism is a common disease, and its molecular mechanism still needs further investigation. Lysine succinylation is found to be involved in various metabolic processes associated with hypothyroidism.
Baoxiang Hu +9 more
doaj +4 more sources
Sirtuin-5 Is Recruited to Hepatic Peroxisomes in Mice Fed Dodecanedioic Acid but Has Little Impact on the Peroxisomal Succinylome [PDF]
BiomoleculesLysine succinylation, and its reversal by sirtuin-5 (SIRT5), is known to modulate mitochondrial fatty acid β-oxidation (FAO). We recently showed that feeding mice dodecanedioic acid, a 12-carbon dicarboxylic acid (DC12) that can be chain-shortened four ...
Yuxun Zhang +9 more
doaj +4 more sources
Sirtuin 5‐Mediated Desuccinylation of ALDH2 Alleviates Mitochondrial Oxidative Stress Following Acetaminophen‐Induced Acute Liver Injury [PDF]
Advanced ScienceAcetaminophen (APAP) overdose is a major cause of drug‐induced liver injury. Sirtuins 5 (SIRT5) has been implicated in the development of various liver diseases. However, its involvement in APAP‐induced acute liver injury (AILI) remains unclear.
Qiwen Yu +14 more
doaj +3 more sources
Protein succinylome analysis identifies citrate synthase as a central regulator of osteoclast metabolic activity. [PDF]
FEBS JRANKL treatment induces expression of the aconitate decarboxylase (ACOD1) in differentiating osteoclasts, which increases succinate levels. The initially high expression of ACOD1 decreases towards the end of differentiation when the levels of the desuccinylase SIRT5 increase. Succinylation of citrate synthase (CS) was detected exclusively in stimulated
Yu D +7 more
europepmc +4 more sources
In-depth analysis of the Sirtuin 5-regulated mouse brain malonylome and succinylome using library-free data-independent acquisitions. [PDF]
Proteomics, 2023 Abstract Post‐translational modifications (PTMs) dynamically regulate proteins and biological pathways, typically through the combined effects of multiple PTMs. Lysine residues are targeted for various PTMs, including malonylation and succinylation. However, PTMs offer specific challenges to mass spectrometry‐based proteomics during data acquisition ...
Bons J +6 more
europepmc +4 more sources
SUCLG2 Regulates Mitochondrial Dysfunction through Succinylation in Lung Adenocarcinoma
Advanced Science, 2023 Mitochondrial dysfunction and abnormal energy metabolism are major features of cancer. However, the mechanisms underlying mitochondrial dysfunction during cancer progression are far from being clarified. Here, it is demonstrated that the expression level
Qifan Hu +10 more
doaj +2 more sources
Systematic analysis of the lysine succinylome in the model medicinal mushroom Ganoderma lucidum [PDF]
BMC Genomics, 2019 Background Ganoderma lucidum, one of the best-known medicinal mushrooms in the world, produces more than 400 different bioactive compounds. However, the regulation of these bioactive compounds biosynthesis is still unclear.
Guangyuan Wang +4 more
doaj +3 more sources
Changes in the Acetylome and Succinylome of Bacillus subtilis in Response to Carbon Source. [PDF]
PLoS ONE, 2015 Lysine residues can be post-translationally modified by various acyl modifications in bacteria and eukarya. Here, we showed that two major acyl modifications, acetylation and succinylation, were changed in response to the carbon source in the Gram ...
Saori Kosono +6 more
doaj +4 more sources
Comprehensive Succinylome Profiling Reveals the Pivotal Role of Lysine Succinylation in Energy Metabolism and Quorum Sensing of Staphylococcus epidermidis [PDF]
Frontiers in Microbiology, 2021 BackgroundLysine succinylation is a newly identified posttranslational modification (PTM), which exists widely from prokaryotes to eukaryotes and participates in various cellular processes, especially in the metabolic processes.
Yiping Zhao +9 more
doaj +3 more sources