Results 51 to 60 of about 15,229 (210)
An improved method for large-scale preparation of negatively and positively supercoiled plasmid DNA
BioTechniques, 2009 A rigorous understanding of the biological function of superhelical tension in cellular DNA requires the development of new tools and model systems for study.
Marita C. Barth +2 more
doaj +1 more source
Quinolones: Mechanism, Lethality and Their Contributions to Antibiotic Resistance
Molecules, 2020 Fluoroquinolones (FQs) are arguably among the most successful antibiotics of recent times. They have enjoyed over 30 years of clinical usage and become essential tools in the armoury of clinical treatments. FQs target the bacterial enzymes DNA gyrase and
Natassja G. Bush +3 more
doaj +1 more source
Chiral theory of DNA supercoiling [PDF]
, 2013 DNA supercoiling is a fundamental biological process occurring in all cells. We developed a theory of braiding (supercoiling) of a pair of DNA molecules that takes into account the contribution of the bending and the electrostatic energy.
Cortini, Ruggero
core +1 more source
Stochastic Entanglement of Deterministic Origami Tentacles For Robust Robotic Grasping
Advanced Science, EarlyView.This study introduces an origami tentacle gripper to robustly grasp objects‐in air, underwater, and even in orbital space‐by exploiting a synergy between local, deterministic deformation programming and global, stochastic entanglements. A single origami tentacle can be designed to coil with a simple tendon pull.
Alec Boron +4 more
wiley +1 more source
Structural interplay between DNA-shape protein recognition and supercoiling: The case of IHF
Computational and Structural Biotechnology Journal, 2022 The integration host factor (IHF) is a prominent example of indirect readout as it imposes one of the strongest bends on relaxed linear DNA. However, the relation between IHF and torsionally constrained DNA, as occurs physiologically, remains unclear. By
George D. Watson +3 more
doaj +1 more source
, 2019 A. A linear double-stranded DNA becomes a topologically constrained molecule if the two ends are covalently joined, forming a circle. Rules of DNA topology are explained using such a molecule (ccc-DNA) in which a numerical parameter called the linking ...
Zhong Qian (8119712) +2 more
core +1 more source
Advanced Science, EarlyView.Corramycin, a myxobacterial natural product antibiotic, exhibits potent bactericidal activity against multidrug‐resistant Mycobacterium tuberculosis. The compound hijacks bacterial transporters such as BacA and OppABCD to enter the cell and inhibits DNA synthesis through a previously unrecognized mode of DNA gyrase poisoning, locking the enzyme in an ...
Franziska Fries +25 more
wiley +1 more source
HU of Streptococcus pneumoniae Is Essential for the Preservation of DNA Supercoiling
Frontiers in Microbiology, 2018 The histone-like protein HU is a conserved nucleoid-associated protein that is involved in the maintenance of the bacterial chromosome architecture. It is the only known nucleoid-associated protein in Streptococcus pneumoniae, but it has not been studied.
María-José Ferrándiz +4 more
doaj +1 more source
Effects of DNA Topology on Transcription from rRNA Promoters in Bacillus subtilis
Microorganisms, 2021 The expression of rRNA is one of the most energetically demanding cellular processes and, as such, it must be stringently controlled. Here, we report that DNA topology, i.e., the level of DNA supercoiling, plays a role in the regulation of Bacillus ...
Petra Sudzinová +5 more
doaj +1 more source
Advanced Science, EarlyView.A PROTAC‐functionalized protein/DNA‐integrated nanoantigen platform is developed to enhance dendritic cell vaccine potency. By combining a VHL‐recruiting peptide‐modified antigen with a sustained expression plasmid within CaCO3 nanoparticles, this system amplifies cross‐presentation, promotes DC maturation, and elicits robust Th1 and CTL responses ...
Peng Liu +4 more
wiley +1 more source