The Urease Gene Cluster of Vibrio parahaemolyticus Does Not Influence the Expression of the Thermostable Direct Hemolysin (TDH) Gene or the TDH‐Related Hemolysin Gene [PDF]
AbstractIn order to investigate why the thermostable direct hemolysin (TDH) and the TDH‐related hemolysin (TRH) of Vibrio parahaemolyticus are produced at low levels from urease‐positive strains, the effect of the functional urease gene cluster of V. parahaemolyticus on the expression of the tdh and trh genes was examined.
Yoshitsugu, Nakaguchi +3 more
openaire +2 more sources
Vibrio parahaemolyticus- An emerging foodborne pathogen [PDF]
Vibrio parahaemolyticus is a halophilic gram negative, motile, oxidase positive, straight or curved rod-shaped, facultative anaerobic bacteria that occur naturally in the marine environment. They form part of the indigenous microflora of aquatic habitats
S Nelapati, K Nelapati, B K Chinnam
doaj
Evaluation of Two Assay Kits for Thermostable Direct Hemolysin (TDH) as an Indicator of TDH‐Related Hemolysin (TRH) Produced by Vibrio parahaemolyticus [PDF]
AbstractReversed passive latex agglutination (RPLA) or enzyme‐linked immunosorbent assay kits with beads (Bead‐ELISA) are commercially available in Japan to detect the thermostable direct hemolysin (TDH) produced by Vibrio parahaemolyticus isolates. We evaluated whether these kits can be used to assay the pathogenic toxin, TDH‐related hemolysin (TRH ...
M, Yoh +4 more
openaire +2 more sources
Distribution and dynamics of epidemic and pandemic Vibrio parahaemolyticus virulence factors
Vibrio parahaemolyticus, autochthonous to estuarine, marine, and coastal environments throughout the world, is the causative agent of food-borne gastroenteritis.
Daniela eCeccarelli +8 more
doaj +1 more source
Efficient Expression and Enzymatic Properties of L-Threonine Dehydrogenase from Escherichia coli [PDF]
Our aim was to improve the catalytic efficiency of L-threonine dehydrogenase (L-TDH) on L-threonine dehydrogenation to synthesize ethyl L-2-aminoacetate. An L-TDH gene from Escherichia coli was mined and solubly expressed in E.
LIU Xinxin, WANG Yao, SHI Hongling, YAO Lunguang, WANG Xian, TANG Cunduo
doaj +1 more source
Aim The study aims to explore the current knowledge and skills of healthcare providers already trained by SIMESON (simulation for essential skills for obstetrical and neonatal care), in reviewing perinatal health situations including current status of ...
Rina Das +10 more
doaj +1 more source
Genetic characterization of trh positive Vibrio spp. isolated from Norway
The thermostable direct hemolysin (TDH) and/or TDH-related hemolysin (TRH) genes are carried by most virulent Vibrio parahaemolyticus serovars. In Norway, trh+ V. parahaemolyticus constitute 4.4% and 4.5 % of the total number of V.
Anette eBauer Ellingsen +4 more
doaj +1 more source
Vibrio parahaemolyticus is one of the significant seafood-borne pathogens causing gastroenteritis in humans. Clustered regularly interspaced short palindromic repeats (CRISPR) are commonly detected in the genomes of V.
Nawaporn Jingjit +3 more
doaj +1 more source
Analysis of the tdh Gene Cloned from a tdh Gene‐ and trh Gene‐Positive Strain of Vibrio parahaemolyticus [PDF]
AbstractA variant of the gene (tdh) encoding thermostable direct hemolysin (TDH) was cloned from the chromosome of Vibrio parahaemolyticus AQ3860, which gave positive results in the hybridization tests with the tdh gene probe and the trh (tdh‐related hemolysin) gene probe and showed a low level of reaction in an enzyme‐linked immunosorbent assay for ...
K, Baba +4 more
openaire +2 more sources
Vibrio parahaemolyticus toxin TDH-A contributes to in vitro cytotoxicity, but not in vivo mortality [PDF]
Vibrio parahaemolyticus toxin TDH-A plays a previously unexplored role in cytotoxicity of HeLa cells, but does not affect mouse mortality using a pulmonary challenge ...
Pineyro, Pablo +4 more
core +3 more sources

