Results 31 to 40 of about 5,570 (173)
Thermolysin in human cultured keratinocyte isolation
Brazilian Journal of BiologyBACKGROUND: When treating extensively burned patients using cultured epidermal sheets, the main problem is the time required for its production. Conventional keratinocyte isolation is usually done using Trypsin. We used a modification of the conventional
A. Gragnani, CS. Sobral, LM. Ferreira
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Mechanism of thermostability in thermolysin – analysis of subsite S2 mutant enzymes of thermolysin [PDF]
Letters in Applied Microbiology, 1999 An aromatic amino acid at position 115 (tryptophan residue; subsite S2) in thermolysin is known to be essential for proteolytic activity of thermolysin. Mutant enzymes substituted by phenylalanine (W115F) and tyrosine (W115Y) at position 115 were expressed at similar levels as the wild type (WT) enzyme in Bacillus subtilis.
M. Kubo +4 more
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Phytotherapy Research, EarlyView.COR stabilizes GCH1 and inhibits ferroptosis. ABSTRACT To investigate the effects and targets of cordycepin (COR) in alleviating non‐alcoholic fatty liver disease (NAFLD). High‐fat diet was used to induce NAFLD in mice, and after COR gavage administration, changes in liver function, glucose and lipid metabolism, and ferroptosis‐related signaling were ...
Chenyang Han +7 more
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Frontiers in Microbiology, 2017 Leptospirosis is a zoonosis caused by spirochetes from the genus Leptospira. This disease is common in tropical and subtropical areas, constituting a serious public health problem.
Thais A. Amamura +4 more
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Hayati Journal of Biosciences, 2010 Three isolates among 138 sponge-associated bacteria were isolated from Waigeo Island, Raja Ampat West Papua Province, Indonesia, have been shown protease inhibitory activity against subtilisin (serine protease), thermolysin (metalloprotease), and crude ...
ARIS TRI WAHYUDI +2 more
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BMJ Open Ophthalmology, 2022 Objective Recent clinical studies have shown that the transplantation of functional retinal pigment epithelium (RPE) cells can prevent the onset of RPE degeneration in age-related macular degeneration.
Diego Ponzin +7 more
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α‐Synuclein strain homogeneity in multiple system atrophy subtypes
Brain Pathology, EarlyView.Two different subtypes of multiple system atrophy are recognized: MSA‐C and MSA‐P. In this manuscript, Lau et al. investigate whether MSA‐C and MSA‐P are caused by different strains of α‐synuclein aggregates. By performing conformational fingerprinting experiments as well as in vitro and in vivo seeding assays, their findings suggest that both MSA‐C ...
Heather H. C. Lau +10 more
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The FEBS Journal, EarlyView.Human NAD(P)H:quinone oxidoreductase 1 is a homodimeric flavoenzyme crucial for redox metabolism and linked to significant health issues. Point mutations at Tyr126 and Tyr128 demonstrate their essential roles in optimizing substrate binding geometry for catalysis, as well as in half‐site reactivity and conformational dynamics during the enzyme's ...
Maribel Rivero +8 more
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Journal of Periodontal Research, EarlyView.A graphical abstract recapping the different sources of dental, periodontal, and other oral‐derived mesenchymal stromal cells (MSCs) and their regenerative mechanisms and potentials. The review's article findings bridge fundamental biological science with translational advances, highlighting the significance of MSCs in craniofacial regenerative ...
Karim M. Fawzy El‐Sayed +6 more
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Fucosylation limits ADCC in clinically used anti‐RhD monoclonal antibodies
Transfusion, EarlyView.Abstract Background Haemolytic disease of the fetus and newborn (HDFN) is caused by maternal alloantibodies, often targeting the D antigen on fetal red blood cells. Maternal immunization is preventable with timely administration of anti‐D polyclonal antibodies (pAbs).
Gabriela Koike +33 more
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