Results 51 to 60 of about 5,570 (173)

Mass Spectrometry Structural Proteomics Enabled by Limited Proteolysis and Cross‐Linking

Mass Spectrometry Reviews, Volume 45, Issue 4, Page 644-671, July/August 2026.
ABSTRACT The exploration of protein structure and function stands at the forefront of life science and represents an ever‐expanding focus in the development of proteomics. As mass spectrometry (MS) offers readout of protein conformational changes at both the protein and peptide levels, MS‐based structural proteomics is making significant strides in the
Haiyan Lu, Zexin Zhu, Lauren Fields, Hua Zhang, Lingjun Li   +4 more
wiley   +1 more source

Induction of Cell-Cell Fusion by Ebola Virus Glycoprotein: Low pH Is Not a Trigger. [PDF]

PLoS Pathogens, 2016
Ebola virus (EBOV) is a highly pathogenic filovirus that causes hemorrhagic fever in humans and animals. Currently, how EBOV fuses its envelope membrane within an endosomal membrane to cause infection is poorly understood.
Ruben M Markosyan, Chunhui Miao, Yi-Min Zheng, Gregory B Melikyan, Shan-Lu Liu, Fredric S Cohen   +5 more
doaj   +1 more source

Safety evaluation of a second extension of use of the food enzyme thermolysin from the non‐genetically modified Anoxybacillus caldiproteolyticus strain AE‐TP

EFSA Journal, Volume 24, Issue 7, July 2026.
Abstract The food enzyme thermolysin (EC. 3.4.24.27) is produced with the non‐genetically modified Anoxybacillus caldiproteolyticus strain AE‐TP by Amano Enzyme Inc. The safety of this food enzyme was evaluated previously, and it did not give rise to safety concerns when used in seven food manufacturing processes.
EFSA Panel on Food Enzymes (FEZ), Holger Zorn, José Manuel Barat Baviera, Claudia Bolognesi, Francesco Catania, Gabriele Gadermaier, Ralf Greiner, Baltasar Mayo, Alicja Mortensen, Yrjö Henrik Roos, Marize L. M. Solano, Henk Van Loveren, Laurence Vernis, Daniele Cavanna, Yi Liu   +14 more
wiley   +1 more source

Screening of Angiotensin-I Converting Enzyme Inhibitory Peptides Derived from Caulerpa lentillifera

Molecules, 2018
Peptides with angiotensin-I converting enzyme (ACE) inhibitory activity have received considerable interest due to their potential as antihypertensive agents and consumer concern over the safety of synthetic drugs.
Cesarea Hulda Joel, Christoper C. Y. Sutopo, Arief Prajitno, Jui-Hsin Su, Jue-Liang Hsu   +4 more
doaj   +1 more source

Limited proteolysis of ribonuclease A with thermolysin in trifluoroethanol [PDF]

Protein Science, 1997
AbstractWe have examined the proteolysis of bovine pancreatic ribonuclease A (RNase) by thermolysin when dissolved in aqueous buffer, pH 7.0, in the presence of 50% (v/v) trifluoroethanol (TFE). Under these solvent conditions, RNase acquires a conformational state characterized by an enhanced content of secondary structure (helix) and reduced tertiary ...
POLVERINO DE LAURETO, PATRIZIA, E. SCARAMELLA, DE FILIPPIS, VINCENZO, M. BRUIX, M. RICO, FONTANA, ANGELO   +5 more
openaire   +3 more sources

Targeting Fatty Acid Synthase With Berberine Promotes IL‐10 Production in Macrophages and Prevents Relapse in a Murine Colitis Model

The FASEB Journal, Volume 40, Issue 11, 15 June 2026.
Berberine enhances IL‐10 production in macrophages via FASN‐dependent mechanisms, thereby promoting epithelial repair and preventing colitis relapse. Created with BioRender.com. ABSTRACT Inflammatory bowel disease (IBD) is a chronic inflammatory disorder characterized by alternating periods of remission and relapse.
Shusaku Hayashi, Yudai Ogawa, Nonoka Wakabayashi, Koga Tokuyama, Makito Oku, Takeshi Yamamoto, Shinichi Kato, Asma Nusrat, Makoto Kadowaki   +8 more
wiley   +1 more source

Denuded Descemet's membrane supports human embryonic stem cell-derived retinal pigment epithelial cell culture.

PLoS ONE, 2023
Recent clinical studies suggest that retinal pigment epithelial (RPE) cell replacement therapy may preserve vision in retinal degenerative diseases. Scaffold-based methods are being tested in ongoing clinical trials for delivering pluripotent-derived RPE
Elena Daniele, Lorenzo Bosio, Noor Ahmed Hussain, Barbara Ferrari, Stefano Ferrari, Vanessa Barbaro, Brian McArdle, Nicolò Rassu, Marco Mura, Francesco Parmeggiani, Diego Ponzin   +10 more
doaj   +1 more source

Assessment of Doxorubicin Internalization and Cytotoxicity in Primary Tumor Spheroids After Collagen Digestion

Current Protocols, Volume 6, Issue 6, June 2026.
Abstract The limited penetration of chemotherapeutic agents into solid tumors remains a major obstacle to effective cancer treatment and is strongly influenced by the extracellular matrix (ECM). Three‐dimensional (3D) tumor spheroids derived from primary tumor cells (PTCs) represent a valuable in vitro model to study how ECM composition and ...
Alessandra Lo Cicero, Gabriele Lo Buglio, Simona Campora, Giulio Ghersi   +3 more
wiley   +1 more source

Chloroplast glycerolipid metabolism during maintenance and under stress

The Plant Journal, Volume 126, Issue 5, June 2026.
SUMMARY Chloroplasts are the defining organelles in green plant tissues, converting light into chemical energy with the help of an intricate and extensive membrane system, the thylakoids. In the process, they release oxygen and assimilate carbon and other elements into molecules that constitute a plant cell's building blocks.
Yosia Mugume, John E. Froehlich, Christoph Benning   +2 more
wiley   +1 more source

Biocatalytic Potential of a Mycobacterial Aminoacylase for Synthesis of N‐Acyl‐L‐Amino Acids in Aqueous Media

ChemBioChem, Volume 27, Issue 7, 14 April 2026.
The aminoacylase MsAA is a suitable enzyme for the biotechnological production of N‐acyl‐L‐amino acids. This study focuses on the optimization of the synthesis of N‐lauroyl‐L‐methionine. A detailed analysis of the catalytic reaction mechanism of MsAA is performed in an in silico protein modeling study.
Jessika Wirges, Gerrit Haeger, Laureline Gennesseaux, Yann Guiavarc’h, Catherine Humeau, Cédric Paris, Isabelle Chevalot, Karl‐Erich Jaeger, Jonas Krapohl, Patrick Schmidt, Johannes Bongaerts, Petra Siegert   +11 more
wiley   +1 more source