Results 41 to 50 of about 5,570 (173)
Semisynthesis of carboxy‐terminal fragments of thermolysin
International Journal of Peptide and Protein Research, 1990 Enzyme‐catalyzed synthesis of two polypeptide fragments, one of which is obtained by chemical synthesis, in the presence of proteolytic enzymes and in aqueous organic solvents constitutes a convenient procedure for the synthesis of proteins and their analogs.
DE FILIPPIS, VINCENZO, FONTANA, ANGELO
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Protein Science, Volume 35, Issue 9, September 2026.Abstract The design of proteins with desired biophysical properties, such as high solubility and low aggregation propensity, is crucial for various biotechnological and biomedical applications. While deep learning‐based methods like ProteinMPNN have shown remarkable success in protein sequence design, their direct output may not always exhibit optimal ...
Sacha T. Larda +2 more
wiley +1 more source
Substrate-dependent Activation of Thermolysin by Salt [PDF]
Bioscience, Biotechnology, and Biochemistry, 2004 Salt-activation of thermolysin was examined using a positively charged fluorescent substrate, (7-methoxycoumarin-4-yl)acetyl-L-Pro-L-Leu-Gly-L-Leu-[N(3)-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH(2) [MOCAc-PLGL(Dpa)AR]. Thermolysin activity increased in a biphasic exponential fashion and was 40 times higher in the presence of 4 M NaCl ...
ONEDA, Hiroshi +2 more
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Evolution of N‐terminal mechanical lability as a determinant for Type III secretion
Protein Science, Volume 35, Issue 8, August 2026.Abstract Many Gram‐negative pathogens critically depend on the Type III secretion system (T3SS) to inject effector proteins into host cells for colonization. Because the channel of the T3SS is narrow (~2 nm), effectors must be unfolded for secretion.
Katherine E. DaPron +7 more
wiley +1 more source
Scientific Reports, 2023 Many people eat polished rice, while rice bran, a by-product known to be rich in protein and expected to have potential functions for health benefits, has not been effectively utilized.
Maiko Shobako +4 more
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ChemMedChem, Volume 21, Issue 13, 14 July 2026.Water influences ligand binding to a protein. Replacing, picking up, or modulating water dynamics restructures the water arrangement and affects the enthalpic and entropic signatures of binding. Whether the two properties add up or cancel each other out determines the expected ligand affinity enhancement.
Gerhard Klebe
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Advanced Science, Volume 13, Issue 38, 9 July 2026.Multidrug‐resistant Vibrio infections are rising rapidly and threaten coastal populations worldwide. This study introduces D‐zp37, a chirality‐engineered antimicrobial peptide with exceptional potency against resistant Vibrio species. D‐zp37 kills planktonic cells, blocks mixed‐species biofilms, disrupts essential bacterial stress responses, and shows ...
Ping Zeng +11 more
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International Journal of Food Properties, 2018 In this study, crude peptide fractions from Hanwoo loins were released by injecting with proteolytic enzymes [no enzymes (control); protease type XIII (E1); thermolysin (E2); and combination of E1 and E2 (E3)] and their bioactivities were determined. The
Kuk-Hwan Seol +5 more
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EFSA JournalThe food enzyme thermolysin (EC. 3.4.24.27) is produced with the non‐genetically modified Anoxybacillus caldiproteolyticus strain AE‐TP by Amano Enzyme Inc. The food enzyme is free from viable cells of the production organism.
EFSA Panel on Food Contact Materials, Enzymes and Processing Aids (CEP) +24 more
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Effects of Radiation Damage on Metal-Binding Sites in Thermolysin
CrystalsRadiation damage is an inherent problem in macromolecular crystallography because it impairs the diffraction quality of crystals and produces inaccurate structural information.
Ki Hyun Nam
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