Comprehensive comparison of the third-generation sequencing tools for bacterial 6mA profiling. [PDF]
Nat CommunDNA N6-methyladenine (6mA) serves as an intrinsic and principal epigenetic marker in prokaryotes, impacting various biological processes. To date, limited advanced sequencing technologies and analyzing tools are available for bacterial DNA 6mA.
Lu B +9 more
europepmc +2 more sources
Comprehensive and deep evaluation of structural variation detection pipelines with third-generation sequencing data. [PDF]
Genome BiolStructural variation (SV) detection methods using third-generation sequencing data are widely employed, yet accurately detecting SVs remains challenging. Different methods often yield inconsistent results for certain SV types, complicating tool selection
Liu Z, Xie Z, Li M.
europepmc +2 more sources
Recovery of human gut microbiota genomes with third-generation sequencing [PDF]
Cell Death and Disease, 2021 Human gut microbiota modulates normal physiological functions, such as maintenance of barrier homeostasis and modulation of metabolism, as well as various chronic diseases including type 2 diabetes and gastrointestinal cancer. Despite decades of research,
Yanfei Li +8 more
doaj +3 more sources
When Livestock Genomes Meet Third-Generation Sequencing Technology: From Opportunities to Applications. [PDF]
Genes (Basel)Third-generation sequencing technology has found widespread application in the genomic, transcriptomic, and epigenetic research of both human and livestock genetics.
Liu X +5 more
europepmc +2 more sources
The third generation sequencing: the advanced approach to genetic diseases
Translational Pediatrics, 2020 Genomic sequencing technologies have revolutionized mutation detection of the genetic diseases in the past few years. In recent years, the third generation sequencing (TGS) has been gaining insight into more genetic diseases owing to the single molecular
T. Xiao, Wenhao Zhou
semanticscholar +4 more sources
Current research status of third-generation sequencing technology in thalassemia detection [PDF]
Frontiers in PediatricsThalassemia is a hereditary hemolytic disorder primarily caused by defects in the hemoglobin genes, which impede the synthesis of hemoglobin peptide chains. This disease is mainly classified into two types: α and β.
Fenglin Zhu +3 more
doaj +2 more sources
Application of third-generation sequencing technology for identifying rare α- and β-globin gene variants in a Southeast Chinese region [PDF]
BMC Medical GenomicsBackground Third-generation sequencing (TGS) based on long-read technology has been gradually used in identifying thalassemia and hemoglobin (Hb) variants.
Jianlong Zhuang +4 more
doaj +2 more sources
Diagnostic value of third-generation nanopore sequencing in extrapulmonary tuberculosis
Frontiers in Cellular and Infection MicrobiologyBackgroundThis study aimed to explore the accuracy of third-generation nanopore sequencing to diagnose extrapulmonary tuberculosis (EPTB).MethodsSamples were collected from the lesions of 67 patients with suspected EPTB admitted between April 2022 and ...
Chang Song +11 more
doaj +3 more sources
Third-Generation Sequencing: The Spearhead towards the Radical Transformation of Modern Genomics
Life, 2021 Although next-generation sequencing (NGS) technology revolutionized sequencing, offering a tremendous sequencing capacity with groundbreaking depth and accuracy, it continues to demonstrate serious limitations.
Konstantina Athanasopoulou +4 more
semanticscholar +1 more source
A window into third generation sequencing [PDF]
Human Molecular Genetics, 2010 First- and second-generation sequencing technologies have led the way in revolutionizing the field of genomics and beyond, motivating an astonishing number of scientific advances, including enabling a more complete understanding of whole genome sequences and the information encoded therein, a more complete characterization of the methylome and ...
Eric E, Schadt +2 more
openaire +3 more sources