Results 81 to 90 of about 71,371 (293)

pBINPLUS/ARS: an improved plant transformation vector based on pBINPLUS

BioTechniques, 2008
Binary plant transformation vectors are widely used for introduction of transgenes into plants via Agrobacterium tumefaciens-mediated transformation.
William R. Belknap, David R. Rockhold, Kent F. McCue   +2 more
doaj   +1 more source

Active Center Control of Termination by RNA Polymerase III and tRNA Gene Transcription Levels In Vivo.

PLoS Genetics, 2016
The ability of RNA polymerase (RNAP) III to efficiently recycle from termination to reinitiation is critical for abundant tRNA production during cellular proliferation, development and cancer.
Keshab Rijal, Richard J Maraia
doaj   +1 more source

Mutational analysis of a yeast transcriptional terminator [PDF]

Trends in Genetics, 1989
We have isolated and mutagenized a DNA fragment from Saccharomyces cerevisiae that specifies mRNA 3' end formation for the convergently transcribed CYC1 and UTR1 genes. An in vivo plasmid supercoiling assay previously showed that this fragment is a transcriptional terminator, and "run-on" assays shown here are consistent with this interpretation.
B I, Osborne, L, Guarente
openaire   +2 more sources

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

Mutant NPM1 in Acute Myeloid Leukemia Initiation and Maintenance

Aging and Cancer, EarlyView.
NPM1 mutations drive acute myeloid leukemia by acting as neomorphic transcriptional regulators that cooperate with Menin–MLL and XPO1 to sustain HOX/MEIS1 expression and block differentiation. Targeting these mutant‐specific transcriptional dependencies provides a rational therapeutic strategy for NPM1‐mutated AML.
Yanan Jiang, Zhong Fan, Runmeng Liu, Xiaotian Zhang   +3 more
wiley   +1 more source

Vectors for cloning and activity determination of the elements of the bacterial transcription system: cloning the transcription terminator of the rRNA operon of the archaebacterium Halobacterium halobium

, 1987
Сконструированы рекомбинантные плазмиды рММ24 и pVR41, содержащие промоторный район Pᵣ бактериофага λ с областями инициации транскрипции и инициации трансляции репрессора λсrо, полилинкер и участок терминации транскрипции бактериофага fd. Показано, что в
Скрипкин, Е.А., Манькин, А.С., Копылов, А.М., Худяков, Ю.Е.   +3 more
core   +1 more source

Transcription termination and antitermination are critical for the fitness and function of the integrative and conjugative element Tn916.

PLoS Genetics
Premature expression of genes in mobile genetic elements can be detrimental to their bacterial hosts. Tn916, the founding member of a large family of integrative and conjugative elements (ICEs; aka conjugative transposons), confers tetracycline ...
Erika S Wirachman, Alan D Grossman
doaj   +1 more source

Developmental and Epileptic Encephalopathy due to Biallelic Pathogenic Variants in PIGM

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective PIGM encodes a critical enzyme in the glycosylphosphatidylinositol (GPI)‐anchor biosynthesis pathway. While promoter‐region mutations in PIGM have been associated with a relatively mild phenotype characterized by portal vein thrombosis and absence seizures, recent evidence suggests that coding‐region mutations result in a more severe
Júlia Sala‐Coromina, Anna Marcé‐Grau, Barbara Masotto, Marta Codina, Lamia BenJemaa, Yasmina Elaribi, Mónica Martinez‐Gallo, Roger Colobran, Angel Sanchez‐Montañez, Irene Valenzuela, Yoshiko Murakami, Alfons Macaya   +11 more
wiley   +1 more source

Single expression cassettes for surface display of ARP3 fused to VSV-G (A); and V5/HA/FLAG-tag (B); and co-expression cassettes where one ARP fragment can be secreted (ARP1) and the other (ARP3) anchored on the cell surface (C); both covalently anchored on the cell surface (D); or both secreted in the medium (E).

, 2014
SP, signal peptide; RBS, ribosomal binding site; P, apf promoter; translational stop codon indicated with an arrowhead and the transcription terminator indicated with a lollipop.
Harold Marcotte (35624), Beatriz Álvarez (3850759), Lennart Hammarström (35623), Yin Lin (421396), Gökçe Günaydın (556537)   +4 more
core   +1 more source

Crystal Structure of Shigella flexneri SF173 Reveals a Dimeric Helical Bundle Conformation

Crystals, 2018
We report the crystal structure and bioinformatic analysis of SF173, a functionally uncharacterized protein from the human enteropathogenic bacteria Shigella flexneri.
Ji-Hun Kim, Hyung-Sik Won, Won-Su Yoon, Seung-Hyeon Seok, Bong-Jun Jung, Seu-Na Lee, Dae-Won Sim, Min-Duk Seo   +7 more
doaj   +1 more source