Results 61 to 70 of about 736,695 (298)

Expression of Shigella flexneri gluQ-rs gene is linked to dksA and controlled by a transcriptional terminator

BMC Microbiology, 2012
Background Glutamyl queuosine-tRNAAsp synthetase (GluQ-RS) is a paralog of the catalytic domain of glutamyl-tRNA synthetase and catalyzes the formation of glutamyl-queuosine on the wobble position of tRNAAsp. Here we analyze the transcription of its gene
Caballero Valeria C, Toledo Viviana P, Maturana Cristian, Fisher Carolyn R, Payne Shelley M, Salazar Juan   +5 more
doaj   +1 more source

Stimulator of interferon genes agonist augmented antitumor immunity of osimertinib in Egfr‐mutated lung cancer

Molecular Oncology, EarlyView.
Combining osimertinib with the STING agonist ADU‐S100 activates innate and adaptive immunity to overcome the non‐inflamed microenvironment of Egfr‐mutant lung cancer. This combination increases NK and CD8+ T‐cell infiltration, associated with activation of the STING‐IRF3 pathway and local immunogenic cell death.
Jun Nishimura, Tadahiro Kuribayashi, Johannes Brägelmann, Sachi Okawa, Masataka Taoka, Shunta Mori, Tomoka Nishimura, Takaaki Tanaka, Go Makimoto, Kiichiro Ninomiya, Kammei Rai, Eiki Ichihara, Ryohei Katayama, Katsuyuki Hotta, Masahiro Tabata, Yosuke Togashi, Yoshinobu Maeda, Martin L. Sos, Katsuyuki Kiura, Kadoaki Ohashi   +19 more
wiley   +1 more source

Dynamic interactions between the promoter and terminator regions of the mammalian BRCA1 gene [PDF]

, 2008
The 85-kb breast cancer-associated gene BRCA1 is an established tumor suppressor gene, but its regulation is poorly understood. We demonstrate by gene conformation analysis in both human cell lines and mouse mammary tissue that gene loops are imposed on ...
Brown, Melissa A., Proudfoot, Nicholas J., Proudfoot, NJ, French, JD, French, Juliet D., Tan-Wong, Sue Mei, Proudfoot, Nicholas, Tan-Wong, SM, Tan-Wong, Sue, Brown, MA   +9 more
core   +1 more source

Mutational Analysis Supports Three-Hairpin Model of Attenuator for Transcription Regulation of ilvBNC Operon in Corynebacterium glutamicum

Microorganisms
The ilvBNC operon in Corynebacterium glutamicum encodes key enzymes for the biosynthesis of branched-chain amino acids (L-isoleucine, L-leucine, and L-valine).
Ludmila E. Ryabchenko, Igor I. Titov, Tatyana E. Leonova, Tatyana I. Kalinina, Tatyana V. Gerasimova, Marina E. Sheremetieva, Nikolay A. Kolchanov, Tamara M. Khlebodarova, Alexander S. Yanenko   +8 more
doaj   +1 more source

Engineering efficient termination of bacteriophage T7 RNA polymerase transcription

G3: Genes, Genomes, Genetics, 2022
The bacteriophage T7 expression system is one of the most prominent transcription systems used in biotechnology and molecular-level research. However, T7 RNA polymerase is prone to read-through transcription due to its high processivity. As a consequence,
Diana G Calvopina-Chavez, Mikaela A Gardner, Joel S Griffitts   +2 more
doaj   +1 more source

Loss of proton‐sensing TDAG8 increases tumor progression in mouse models of colon cancer

Molecular Oncology, EarlyView.
Loss of the pH‐sensing receptor TDAG8 accelerates colorectal cancer progression in mice. Animals lacking TDAG8 expression had increased tumor growth, DNA damage, and recruitment of tumor‐associated immune cells, including macrophages, neutrophils, and monocytes.
Ermanno Malagola, Yasmine Illi, Anna Bircher, Marijn Wilmink, Rachele F. Malagutti, Solenn Ottié, Cordelia Schuler, Pedro A. Ruiz, Cheryl de Vallière, Klaus Seuwen, Gerhard Rogler, Martin Hausmann   +11 more
wiley   +1 more source

Antisense transcription as a tool to tune gene expression

Molecular Systems Biology, 2016
A surprise that has emerged from transcriptomics is the prevalence of genomic antisense transcription, which occurs counter to gene orientation. While frequent, the roles of antisense transcription in regulation are poorly understood.
Jennifer AN Brophy, Christopher A Voigt
doaj   +1 more source

Castration‐resistant prostate cancer cells are addicted to the high activity of cyclin‐dependent kinase 2

Molecular Oncology, EarlyView.
We show that emergence of castration‐resistant prostate (CRPC) is associated with significant upregulation of cyclins that positively regulate cyclin‐dependent kinase 2 (CDK2) and concomitant downregulation of CDK4 cyclins. This renders CRPC cells dependent on the high activity of CDK2, and CDK2 inhibitors synergistically sensitize CRPC cells to both ...
Joyeeta Chatterjee, Alessia Marin, Shivani Yalala, Harri M. Itkonen   +3 more
wiley   +1 more source

Mechanism of catabolite repression in the bgl operon of Escherichia coli: involvement of the anti-terminator BglG, CRP-cAMP and EIIA^Glc in mediating glucose effect downstream of transcription initiation [PDF]

, 2000
Background: Expression of the bgl operon of Escherichia coli, involved in the regulated uptake and utilization of aromatic β-glucosides, is extremely sensitive to the presence of glucose in the growth medium.
Gulati, Abhilasha, Mahadevan, Subramony
core   +1 more source

Characterization of the dual regulation by a c-di-GMP riboswitch Bc1 with a long expression platform from Bacillus thuringiensis

Microbiology Spectrum
A riboswitch generally regulates the expression of its downstream genes through conformational change in its expression platform (EP) upon ligand binding.
Lu Liu, Dehua Luo, Yongji Zhang, Dingqi Liu, Kang Yin, Qing Tang, Shan-Ho Chou, Jin He   +7 more
doaj   +1 more source