Results 51 to 60 of about 71,371 (293)

Interaction surface of bacteriophage P4 protein Psu required for complex formation with the transcription terminator Rho

, 2009
Rho-dependent transcription termination is an essential function in prokaryotes, and the transcription terminator Rho is highly conserved among different species.
Sen, Ranjan, Pani, Bibhusita, Ranjan, Amitabh   +2 more
core   +1 more source

Expression of Shigella flexneri gluQ-rs gene is linked to dksA and controlled by a transcriptional terminator

BMC Microbiology, 2012
Background Glutamyl queuosine-tRNAAsp synthetase (GluQ-RS) is a paralog of the catalytic domain of glutamyl-tRNA synthetase and catalyzes the formation of glutamyl-queuosine on the wobble position of tRNAAsp. Here we analyze the transcription of its gene
Caballero Valeria C, Toledo Viviana P, Maturana Cristian, Fisher Carolyn R, Payne Shelley M, Salazar Juan   +5 more
doaj   +1 more source

PARK(ing) time–How park deficiency affects the biological clock in a Drosophila model of Parkinson's disease

FEBS Letters, EarlyView.
Drosophila park mutants serve as a model for Parkinson's disease. We used this strain to investigate the connection between oxidative stress and the circadian clock mechanism. We showed that increased oxidative stress affects the physiology of pacemaker cells, disrupting their daily structural plasticity. Lack of rhythmic signaling from pacemaker cells
Kamila Zientara, Kinga Skoczek, Elzbieta Pyza, Milena Damulewicz   +3 more
wiley   +1 more source

Structural insights and therapeutic targets in Acinetobacter baumannii capsule biosynthesis

FEBS Letters, EarlyView.
Hypervirulent KL49 A. baumannii's capsular polysaccharide contains the nonulosonic acid 8‐epi‐Leg5,7Ac2, synthesized by epimerization via ElaA, ElaB, and ElaC. Crystal structures of ElaA, ElaB, and ElaC reveal their role in CMP‐Leg5,7Ac2 synthesis and regioselective C8 epimerization.
Woo Cheol Lee, Junho Jeong, Chae Yeong Lee, Hyunjoon Cho, Man Hwan Oh, Je Chul Lee, Jee‐Young Lee, Yangmee Kim   +7 more
wiley   +1 more source

Engineering efficient termination of bacteriophage T7 RNA polymerase transcription

G3: Genes, Genomes, Genetics, 2022
The bacteriophage T7 expression system is one of the most prominent transcription systems used in biotechnology and molecular-level research. However, T7 RNA polymerase is prone to read-through transcription due to its high processivity. As a consequence,
Diana G Calvopina-Chavez, Mikaela A Gardner, Joel S Griffitts   +2 more
doaj   +1 more source

Three phosphatase families form a community: The phosphohydrolases that act upon inositol pyrophosphates

FEBS Letters, EarlyView.
Inositol pyrophosphates are energy‐rich signaling molecules that perform critical functions in cells. Three different families of phosphatases hydrolyze the β phosphate of the inositol pyrophosphate molecules: two have narrow specificities and one is promiscuous.
Ronda J. Rolfes
wiley   +1 more source

Antisense transcription as a tool to tune gene expression

Molecular Systems Biology, 2016
A surprise that has emerged from transcriptomics is the prevalence of genomic antisense transcription, which occurs counter to gene orientation. While frequent, the roles of antisense transcription in regulation are poorly understood.
Jennifer AN Brophy, Christopher A Voigt
doaj   +1 more source

Genetic organization of the rex locus (gbs1167) and similarity to other Rex proteins.

, 2021
A Rho-independent transcription terminator, is present downstream of gbs1164-1166 operon. Arrow represents the transcriptional start site of the gbs1167 gene. gbs1168 encodes a radC-like gene. Percentage of identity and protein size (number of amino acid
Derek T. Logan (375763), Saravanamuthu Thiyagarajan (9604784), Philippe Glaser (380391), Annika Rogstam (11250638), Violette Da Cunha (380388), Aurelie Derré-Bobillot (11250644), Glen C. Ulett (11250650), Thierry Franza (11250635), Mikael C. Bauer (2435650), Claes von Wachenfeldt (11250653), Matthew J. Sullivan (11250641), Kelvin G. K. Goh (11250647), Philippe Gaudu (249956)   +12 more
core   +1 more source

Heterozygous loss‐of‐function alleles associate the conserved 3′‐5′ exoribonuclease EXOSC10 with hypersensitivity to the anticancer drug 5‐fluorouracil

Molecular Oncology, EarlyView.
EXOSC10, an essential nuclear RNA exosome‐associated 3′‐5′ exoribonuclease, is inhibited by the anticancer drug 5‐fluorouracil (5‐FU), and EXOSC10 depletion increases 5‐FU sensitivity. The colon‐cancer variant EXOSC10S402T, located in a proteolysis motif, is stable and nuclear but nonfunctional in vivo.
Radhika Sain, Yann Le Page, Frédéric Percevault, Emmanuelle Becker, Luc Negroni, Almudena Fernandez, Marta Cantero, Diego Muñoz‐Santos, Lluís Montoliu, Cyrille Garnier, Michael Primig   +10 more
wiley   +1 more source

Characterization of the dual regulation by a c-di-GMP riboswitch Bc1 with a long expression platform from Bacillus thuringiensis

Microbiology Spectrum
A riboswitch generally regulates the expression of its downstream genes through conformational change in its expression platform (EP) upon ligand binding.
Lu Liu, Dehua Luo, Yongji Zhang, Dingqi Liu, Kang Yin, Qing Tang, Shan-Ho Chou, Jin He   +7 more
doaj   +1 more source