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Virus‐Induced Gene Silencing, a Post Transcriptional Gene Silencing Method [PDF]
International Journal of Plant Genomics, 2009 Virus‐induced gene silencing (VIGS) is one of the reverse genetics tools for analysis of gene function that uses viral vectors carrying a target gene fragment to produce dsRNA which trigger RNA‐mediated gene silencing. There are a number of viruses which have been modified to silence the gene of interest effectively with a sequence‐specific manner ...
Unver, Turgay, Budak, Hikmet
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Characteristics of post‐transcriptional gene silencing [PDF]
EMBO reports, 2001 A number of gene silencing phenomena that inactivate genes at the post‐transcriptional level have been identified. Due to its potential for studying gene function, post‐transcriptional gene silencing (PTGS) has become an intense area of research. In this review we describe the different means of inducing PTGS and discuss the possible biological roles ...
A. Chicas, MACINO, Giuseppe
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Transgene-induced gene silencing is not affected by a change in ploidy level. [PDF]
PLoS ONE, 2008 BACKGROUND: Whole genome duplication, which results in polyploidy, is a common feature of plant populations and a recurring event in the evolution of flowering plants.
Daniela Pignatta +4 more
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Non-Coding RNA, 2021 RNA silencing pathways control eukaryotic gene expression transcriptionally or posttranscriptionally in a sequence-specific manner. In RNA silencing, the production of double-stranded RNA (dsRNA) gives rise to various classes of 20–24 nucleotide (nt ...
Xia Hua +5 more
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Molecular Plant-Microbe Interactions, 2003 Promoters isolated from the Tomato leaf curl virus (TLCV) drive both constitutive and tissue-specific expression in transgenic tobacco. Following systemic TLCV infection of plants stably expressing TLCV promoter:GUS transgenes, transgene expression ...
Mark Seemanpillai +3 more
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Epigenetic silencing in transgenic plants
Frontiers in Plant Science, 2015 Epigenetic silencing is a natural phenomenon in which the expression of gene is regulated through modifications of DNA, RNA or histone proteins. It is a mechanism for defending host genomes against the effects of transposable element, viral infection and
Sarma eRajeev Kumar +2 more
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The Whys and Wherefores of Transitivity in Plants
Frontiers in Plant Science, 2020 Transitivity in plants is a mechanism that produces secondary small interfering RNAs (siRNAs) from a transcript targeted by primary small RNAs (sRNAs). It expands the silencing signal to additional sequences of the transcript.
Felipe F. de Felippes +1 more
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The SCFDia2 ubiquitin E3 ligase ubiquitylates Sir4 and functions in transcriptional silencing. [PDF]
PLoS Genetics, 2012 In budding yeast, transcriptional silencing, which is important to regulate gene expression and maintain genome integrity, requires silent information regulator (Sir) proteins.
Rebecca J Burgess +4 more
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Reconstitution of Heterochromatin-Dependent Transcriptional Gene Silencing [PDF]
Molecular Cell, 2009 Heterochromatin assembly in budding yeast requires the SIR complex, which contains the NAD-dependent deacetylase Sir2 and the Sir3 and Sir4 proteins. Sir3 binds to nucleosomes containing deacetylated histone H4 lysine 16 (H4K16) and, with Sir4, promotes spreading of Sir2 and deacetylation along the chromatin fiber.
Johnson, Aaron +5 more
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A Method to Produce vsiRNAs in Plants with Cross-Kingdom Gene Silencing Capacity
Applied Sciences, 2022 Plants have evolved defense mechanisms to suppress viral transcription and replication by transcriptional and post-transcriptional gene silencing mediated by virus-derived small interfering RNAs (vsiRNAs).
Hernán de Jesús Villanueva-Alonzo +11 more
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