Results 91 to 100 of about 304,117 (294)

Mutant p53R273H disrupts PDPK1 homodimerization and contributes to PDPK1 activation

Molecular Oncology, EarlyView.
How mutant p53R273H drives AKT signaling is unclear. We show that p53R273H, but not wild‐type, directly binds PDPK1 via a mutation‐dependent conformational change. This interaction disrupts inhibitory PDPK1 homodimerization and enhances AKT phosphorylation.
Mei Chee Lim, Alvin Kunyao Guo, Yoko Itahana, Srinivasaraghavan Kannan, Valerie Goh, Mingmin Ong, Terina Yan Wen Tay, Weiying Ye, Sabhashina Radha Krishnan, Jun Nishiyama, Chandra S Verma, Koji Itahana   +11 more
wiley   +1 more source

Enhancing the potency of in vivo lentiviral vector mediated gene therapy to hepatocytes

Nature Communications
In vivo gene therapy to the liver using lentiviral vectors (LV) may represent a one-and-done therapeutic approach for monogenic diseases. Increasing LV gene therapy potency is crucial for reducing the effective doses, thus alleviating dose-dependent ...
Cesare Canepari, Michela Milani, Chiara Simoni, Francesco Starinieri, Monica Volpin, Anna Fabiano, Mauro Biffi, Fabio Russo, Rossana Norata, Martina Rocchi, Chiara Brombin, Federica Cugnata, Eugenio Montini, Francesca Sanvito, Markus Grompe, Alessio Cantore   +15 more
doaj   +1 more source

An in vivo systemic massively parallel platform for deciphering animal tissue-specific regulatory function

Frontiers in Genetics
Introduction: Transcriptional regulation is an important process wherein non-protein coding enhancer sequences play a key role in determining cell type identity and phenotypic diversity.
Ashley R. Brown, Ashley R. Brown, Grant A. Fox, Grant A. Fox, Irene M. Kaplow, Irene M. Kaplow, Irene M. Kaplow, Alyssa J. Lawler, Alyssa J. Lawler, BaDoi N. Phan, BaDoi N. Phan, BaDoi N. Phan, Lahari Gadey, Lahari Gadey, Morgan E. Wirthlin, Morgan E. Wirthlin, Easwaran Ramamurthy, Easwaran Ramamurthy, Gemma E. May, Ziheng Chen, Qiao Su, Qiao Su, C. Joel McManus, Robert van de Weerd, Robert van de Weerd, Andreas R. Pfenning, Andreas R. Pfenning, Andreas R. Pfenning   +27 more
doaj   +1 more source

Near-field optomechanical transduction enhanced by Raman gain

Optics Express
Raman-gain-enhanced near-field optomechanical transduction between a movable optical cavity and SiN-membrane resonator is demonstrated. The Raman gain compensates for the intrinsic loss of the cavity and amplifies the optomechanical transduction, through which the membrane vibration is sensed using a high-Q whispering-gallery-mode optical cavity ...
Ryoko Sakuma, Motoki Asano, Hiroshi Yamaguchi, Hajime Okamoto   +3 more
openaire   +3 more sources

DataSheet_2_A novel signature constructed by super-enhancer-related genes for the prediction of prognosis in hepatocellular carcinoma and associated with immune infiltration.xlsx

, 2023
BackgroundSuper-enhancer (SE) refers to a regulatory element with super transcriptional activity, which can enrich transcription factors and drive gene expression. SE-related genes play an important role in the pathogenesis of malignant tumors, including
Qian Guo (107009), Hongping Yu (188118), Xueyan Wei (4401322), Yingchun Liu (3875), Yanji Jiang (9526885), Qiuling Lin (9526882), Zihan Zhou (4184407), Peiqin Chen (10644699), Meiying Long (13999779), Moqin Qiu (9526879), Jialin Qiu (5081603), Cunli Nong (14590328), Runwei Li (1948711), Qiuping Wen (13999782), Qiongguang Huang (13581310), Xianguo Zhou (9526897)   +15 more
core   +1 more source

Targeting the EpCAM‐AXL axis to overcome drug resistance in lung cancer

Molecular Oncology, EarlyView.
Lung cancer cells often evade therapy by hijacking signaling pathways. We reveal that cleaved EpCAM (sEpCAM) stabilizes the oncogenic protein AXL, driving NF‐κB and STAT3‐mediated chemoresistance. This EpCAM‐AXL axis identifies a high‐risk patient subset with poor prognosis.
Alexa Guerrero‐Alba, Mark Shacker, Timothy P. Fleming, Michael A. Smith, Ross M. Bremner, Narendra V. Sankpal   +5 more
wiley   +1 more source

Lentiviral vectors for precise expression to treat X-linked lymphoproliferative disease

Molecular Therapy: Methods & Clinical Development
X-linked lymphoproliferative disease (XLP1) results from SH2D1A gene mutations affecting the SLAM-associated protein (SAP). A regulated lentiviral vector (LV), XLP-SMART LV, designed to express SAP at therapeutic levels in T, NK, and NKT cells, is ...
Paul G. Ayoub, Julia Gensheimer, Lindsay Lathrop, Colin Juett, Jason Quintos, Kevin Tam, Jack Reid, Feiyang Ma, Curtis Tam, Grace E. McAuley, Devin Brown, Xiaomeng Wu, Ruixue Zhang, Kathryn Bradford, Roger P. Hollis, Gay M. Crooks, Donald B. Kohn   +16 more
doaj   +1 more source

Identification of super-enhancer-associated transcription factors regulating glucose metabolism in poorly differentiated thyroid carcinoma

, 2022
This study aimed to uncover transcription factors that regulate super-enhancers involved in glucose metabolism reprogramming in poorly differentiated thyroid carcinoma (PDTC).
Yongrui Du (13826368), Xuexia Lin (1778455), Kun Liu (148201), Hui Li (32376)   +3 more
core   +1 more source

Engineering IL‐4 resistant proinflammatory human myeloid cells for cancer immunotherapy

Molecular Oncology, EarlyView.
We developed a scalable workflow to generate proinflammatory human myeloid cells. CRISPR/Cas9‐edited CD34+ hematopoietic stem and progenitor cells were expanded and differentiated with M‐CSF. Deletion of STAT6 or STAT6/NFKB1 enhanced macrophage proinflammatory gene expression and cytokine secretion in the presence of IL‐4 while maintaining antibody ...
Theresa Barberi, Alan D. Friedman
wiley   +1 more source

Spatial and longitudinal tracking of enhancer-AAV vectors that target transgene expression to injured mouse myocardium

eLife
Tissue regeneration enhancer elements (TREEs) direct expression of target genes in injured and regenerating tissues. Additionally, TREEs of zebrafish origin were shown to direct expression of transgenes in border zone regions after cardiac injury when ...
David W Wolfson, Joshua A Hull, Yongwu Li, Trevor J Gonzalez, Mourya D Jayaram, Garth W Devlin, Valentina Cigliola, Kelsey A Oonk, Alan Rosales, Nenad Bursac, Aravind Asokan, Kenneth D Poss   +11 more
doaj   +1 more source