Results 11 to 20 of about 262,707 (218)

Molecular engineering of individual dye-based nanoparticle photostability for ultrabright two-photon fluorescence [PDF]

Beilstein Journal of Nanotechnology
Dye-based fluorescent organic nanoparticles (dFONs) represent a promising class of bioimaging probes combining high brightness with molecular tunability.
Eleonore Kurek, Sasha Cooper, Alexandre Clausolles, Karen Perronet, Jonathan Daniel, Mireille Blanchard-Desce, François Marquier   +6 more
doaj   +2 more sources

Place Cells in Head-Fixed Mice Navigating a Floating Real-World Environment

Frontiers in Cellular Neuroscience, 2021
The hippocampal place cell system in rodents has provided a major paradigm for the scientific investigation of memory function and dysfunction. Place cells have been observed in area CA1 of the hippocampus of both freely moving animals, and of head-fixed
Mary Ann Go, Jake Rogers, Giuseppe P. Gava, Catherine E. Davey, Catherine E. Davey, Seigfred Prado, Yu Liu, Simon R. Schultz   +7 more
doaj   +1 more source

Accurate spike estimation from noisy calcium signals for ultrafast three-dimensional imaging of large neuronal populations in vivo

Nature Communications, 2016
Two-photon laser scanning microscopy allows functional calcium imaging of large neuronal populations in vivo, but the recorded signals typically suffer from low signal to noise.
Thomas Deneux, Attila Kaszas, Gergely Szalay, Gergely Katona, Tamás Lakner, Amiram Grinvald, Balázs Rózsa, Ivo Vanzetta   +7 more
doaj   +1 more source

Multimodal Characterization of Seizures in Zebrafish Larvae

Biomedicines, 2022
Epilepsy accounts for a significant proportion of the world’s disease burden. Indeed, many research efforts are produced both to investigate the basic mechanism ruling its genesis and to find more effective therapies.
Lapo Turrini, Michele Sorelli, Giuseppe de Vito, Caterina Credi, Natascia Tiso, Francesco Vanzi, Francesco Saverio Pavone   +6 more
doaj   +1 more source

Microglia protect against brain injury and their selective elimination dysregulates neuronal network activity after stroke

Nature Communications, 2016
How microglia contribute to brain injury or repair is unclear. Here combining microglia manipulations and calcium imaging, the authors show that selective elimination of microglia leads to disrupted neuronal calcium dynamics and markedly increased brain ...
Gergely Szalay, Bernadett Martinecz, Nikolett Lénárt, Zsuzsanna Környei, Barbara Orsolits, Linda Judák, Eszter Császár, Rebeka Fekete, Brian L. West, Gergely Katona, Balázs Rózsa, Ádám Dénes   +11 more
doaj   +1 more source

Improving Focal Photostimulation of Cortical Neurons with Pre-derived Wavefront Correction

Frontiers in Cellular Neuroscience, 2017
Recent progress in neuroscience to image and investigate brain function has been made possible by impressive developments in optogenetic and opto-molecular tools.
Vincent R. Daria, Julian M. C. Choy, Sharmila S. Sané, Woei M. Lee, Christian Stricker, Christian Stricker, Hans A. Bachor   +6 more
doaj   +1 more source

A New Two-Photon Ratiometric Fluorescent Probe for Detecting Alkaline Phosphatase in Living Cells

Molecules, 2016
Alkaline phosphatase (ALP) is an important diagnostic indicator of many human diseases. To quantitatively track ALP in biosystems, herein, for the first time, we report an efficient two-photon ratiometric fluorescent probe, termed probe 1 and based on ...
Xiaohong Zhou, Yuren Jiang, Xiongjie Zhao, Yao Zhu   +3 more
doaj   +1 more source

RETRACTED: An acridone-derived fluorescent off-on probe for detection and in vivo imaging of nitroreductase

Frontiers in Materials, 2023
Understanding the functions of enzymes in various physiological processes is important, but the design of signaling probes for fast analysis of enzymatic activity is particularly challenging.
Meicen Liu, Guanyu Chen, Guanyu Chen, Lilan Xu, Lilan Xu, Tingting Chen, Tingting Chen, Wenqian Chen, Wenqian Chen, Jinghua Chen, Jinghua Chen, Xi Zhang   +11 more
doaj   +1 more source

Multi-neuronal recording in unrestrained animals with all acousto-optic random-access line-scanning two-photon microscopy

Frontiers in Neuroscience, 2023
To understand how neural activity encodes and coordinates behavior, it is desirable to record multi-neuronal activity in freely behaving animals. Imaging in unrestrained animals is challenging, especially for those, like larval Drosophila melanogaster ...
Akihiro Yamaguchi, Rui Wu, Paul McNulty, Doycho Karagyozov, Mirna Mihovilovic Skanata, Marc Gershow, Marc Gershow, Marc Gershow   +7 more
doaj   +1 more source

Simultaneous two-photon imaging and two-photon optogenetics of cortical circuits in three dimensions

eLife, 2018
The simultaneous imaging and manipulating of neural activity could enable the functional dissection of neural circuits. Here we have combined two-photon optogenetics with simultaneous volumetric two-photon calcium imaging to measure and manipulate neural
Weijian Yang, Luis Carrillo-Reid, Yuki Bando, Darcy S Peterka, Rafael Yuste   +4 more
doaj   +1 more source