Results 81 to 90 of about 4,897 (216)

Recombinant Monoclonal Antibodies for Detecting the Tubulin Post‐Translational Modifications Glutamylation and Lysine‐40 Acetylation

Cytoskeleton, EarlyView.
ABSTRACT Post‐translational modifications (PTMs) to tubulin subunits in microtubule filaments are thought to comprise a component of the tubulin code that specifies microtubule functions in cell physiology and animal development. Acetylation of Lysine‐40 (K40) on α‐tubulin (αTub‐K40ac) and glutamylation of both α‐ and β‐tubulin are two tubulin PTMs of ...
Lynne Blasius, Ezekiel C. Thomas, Erik H. Collet, Erin Jenson, Chad G. Pearson, Ryoma Ohi, Kristen J. Verhey   +6 more
wiley   +1 more source

Predicting Knot and Catenane Type of Products of Site-specific Recombination on Twist Knot Substrates [PDF]

, 2011
Site-specific recombination on supercoiled circular DNA molecules can yield a variety of knots and catenanes. Twist knots are some of the most common conformations of these products and they can act as substrates for further rounds of site-specific ...
Buck, Dorothy, Valencia, Karin
core  

Expression of mutant TIE2 p.L914F during mouse development causes embryonic lethality and defects in vascular remodeling

Developmental Dynamics, EarlyView.
Abstract Background Sporadic venous malformation (VM) is associated with the hyperactivating p.L914F mutation in TIE2, a receptor tyrosine kinase essential for vascular development. This mutation is not found in hereditary VM, suggesting incompatibility with life when expressed during early vascular development.
Lindsay J. Bischoff, Sandra Schrenk, Kara Soroko, Chhiring Sherpa, Ashok Arasu, Damien Reynaud, Elisa Boscolo   +6 more
wiley   +1 more source

Attachment Site Selection and Identity in Bxb1 Serine Integrase-Mediated Site-Specific Recombination [PDF]

, 2013
Phage-encoded serine integrases mediate directionally regulated site-specific recombination between short attP and attB DNA sites without host factor requirements.
Ghosh, P, Hatfull, GF, Singh, S
core   +3 more sources

Mutational Analysis of the Archaeal Tyrosine Recombinase SSV1 Integrase Suggests a Mechanism of DNA Cleavage in trans [PDF]

Journal of Biological Chemistry, 2004
The only tyrosine recombinase so far studied in archaea, the SSV1 integrase, harbors several changes in the canonical residues forming the catalytic pocket of this family of recombinases. This raised the possibility of a different mechanism for archaeal tyrosine recombinase.
Claire, Letzelter, Michel, Duguet, Marie-Claude, Serre   +2 more
openaire   +2 more sources

Two different evolutionary lines of filamentous phages in Ralstonia solanacearum: their effects on bacterial virulence [PDF]

, 2015
The integration and excision of various filamentous phage genomes into and out of their host chromosomes occurs by site-specific recombination. The mechanisms proposed for these events include reactions mediated by phage-encoded recombinases and host ...
Ahmed Askora, Takashi Yamada
core   +2 more sources

The evolution of tyrosine-recombinase elements in [PDF]

, 2014
Transposable elements can be categorised into DNA and RNA elements based on their mechanism of transposition. Tyrosine recombinase elements (YREs) are relatively rare and poorly understood, despite sharing characteristics with both DNA and RNA elements.
Szitenberg, Amir, Koutsovoulos, Georgios, Blaxter, Mark L., Lunt, David H.   +3 more
openaire  

Unveiling Hidden Catalytic Contributions of the Conserved His/Trp-III in Tyrosine Recombinases: Assembly of a Novel Active Site in Flp Recombinase Harboring Alanine at this Position [PDF]

Journal of Molecular Biology, 2007
The catalytic pentad of tyrosine recombinases, that assists the tyrosine nucleophile, includes a conserved histidine/tryptophan (His/Trp-III). Flp and Cre harbor tryptophan at this position; most of their kin recombinases display histidine. Contrary to the conservation rule, Flp(W330F) is a much stronger recombinase than Flp(W330H).
Chien-Hui, Ma, Agnieszka, Kwiatek, Swetha, Bolusani, Yuri, Voziyanov, Makkuni, Jayaram   +4 more
openaire   +2 more sources

An intracellular recombinant single‐chain variable antibody fragment as a new class of phosphodiesterase type 5 inhibitors

British Journal of Pharmacology, EarlyView.
Background and Purpose Cyclic guanosine monophosphate (cGMP) is a ubiquitous second messenger involved in human (patho‐)physiology. Phosphodiesterase 5 (PDE5) is a major cGMP hydrolyzing enzyme in many cell types including vascular smooth muscle cells (VSMCs). Several highly selective PDE5 inhibitors are in clinical use. However, there are currently no
Kürsat Kirkgöz, Sophie Sprenger, Olga Schweigert, Sanika Mohagaonkar, Matti Walsdorff, Tanja Zeller, Filip Berisha, Viacheslav O. Nikolaev, Sergei D. Rybalkin   +8 more
wiley   +1 more source

Conservation of structure and function among tyrosine recombinases: homology-based modeling of the lambda integrase core-binding domain [PDF]

Nucleic Acids Research, 2003
Tyrosine recombinases participate in diverse biological processes by catalyzing recombination between specific DNA sites. Although a conserved protein fold has been described for the catalytic (CAT) domains of five recombinases, structural relationships between their core-binding (CB) domains remain unclear.
Brian M, Swalla, Richard I, Gumport, Jeffrey F, Gardner   +2 more
openaire   +2 more sources