Turnover of Variant Surface Glycoprotein in Trypanosoma brucei Is Not Altered in Response to Specific Silencing [PDF]
mSphere, 2022 African trypanosomes evade the immune system of the mammalian host by the antigenic variation of the predominant glycosylphosphatidylinositol (GPI)-anchored surface protein, variant surface glycoprotein (VSG).
Mohamed Sharif +3 more
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Turnover of Variant Surface Glycoprotein in
mBio, 2021 African trypanosomes utilize glycosylphosphatidylinositol (GPI)-anchored variant surface glycoprotein (VSG) to evade the host immune system. VSG turnover is thought to be mediated via cleavage of the GPI anchor by endogenous GPI-specific phospholipase C (
Paige Garrison +9 more
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Transcription Dependent Loss of an Ectopically Expressed Variant Surface Glycoprotein during Antigenic Variation in Trypanosoma brucei [PDF]
mBio, 2022 In the mammalian host, Trypanosoma brucei is coated in a single-variant surface glycoprotein (VSG) species. Stochastic switching of the expressed VSG allows the parasite to escape detection by the host immune system.
Emilia Jane McLaughlin +3 more
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Monoallelic expression and epigenetic inheritance sustained by a Trypanosoma brucei variant surface glycoprotein exclusion complex [PDF]
Nature Communications, 2019 Monoallelic expression of variant surface glycoprotein genes (VSGs) is essential for immune evasion by Trypanosoma brucei. Here, Faria et al. show that the VEX protein complex controls VSG allelic exclusion, and that CAF‐1 sustains inheritance of the VEX‐
Joana Faria +5 more
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Variant surface glycoprotein density defines an immune evasion threshold for African trypanosomes undergoing antigenic variation [PDF]
Nature Communications, 2017 Trypanosoma brucei evades the host immune system through replacement of a variant surface glycoprotein (VSG) coat. Here, the authors show that VSG replacement takes several days to complete, and the parasite is vulnerable to the host immune system for a ...
Jason Pinger +2 more
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Chaperone requirements for biosynthesis of the trypanosome variant surface glycoprotein. [PDF]
PLoS ONE, 2010 Trypanosoma brucei does not respond transcriptionally to several endoplasmic reticulum (ER) stress conditions, including tunicamycin or dithiothreitol, indicating the absence of a conventional unfolded protein response. This suggests divergent mechanisms
Mark C Field +4 more
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The release of host-derived antibodies bound to the variant surface glycoprotein (VSG) of Trypanosoma brucei cannot be explained by pH-dependent conformational changes of the VSG dimer [version 1; peer review: 2 approved] [PDF]
Open Research EuropeBackground Trypanosoma brucei is a protozoan parasite that evades the mammalian host’s adaptive immune response by antigenic variation of the highly immunogenic variant surface glycoprotein (VSG).
Pavel Nesterov +8 more
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Expression of a specific variant surface glycoprotein has a major impact on suramin sensitivity and endocytosis in Trypanosoma brucei [PDF]
FASEB BioAdvances, 2019 Suramin was introduced into the clinic a century ago and is still used to treat the first stage of acute human sleeping sickness. Due to its size and sixfold negative charge, uptake is mediated through endocytosis and the suramin receptor in trypanosomes
Natalie Wiedemar +5 more
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vsgseq2: an updated pipeline for analysis of the diversity and abundance of population-wide Trypanosoma brucei VSG expression [version 1; peer review: 2 approved] [PDF]
Wellcome Open ResearchTrypanosoma brucei is an extracellular eukaryotic parasite that causes sleeping sickness in humans and Nagana, Surra and Dourine in livestock, game animals and horses.
Stephen Larcombe +4 more
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VEX1 Influences mVSG Expression During the Transition to Mammalian Infectivity in Trypanosoma brucei
Frontiers in Cell and Developmental Biology, 2022 The Trypanosoma (T) brucei life cycle alternates between the tsetse fly vector and the mammalian host. In the insect, T. brucei undergoes several developmental stages until it reaches the salivary gland and differentiates into the metacyclic form, which ...
Eliane Tihon +6 more
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