Results 151 to 160 of about 1,385 (169)

High-affinity VNARs targeting human hemoglobin: Screening, stability and binding analysis

International Journal of Biological Macromolecules
Hemoglobin, composed of α- and β-chains, is essential for oxygen transport and is key in diagnosing and treating gastrointestinal and blood disorders. It also aids in detecting blood contamination and estimating transfusion volumes. Immunological methods, based on antigen-antibody interactions, are distinguished by their high sensitivity and accuracy ...
Wen-Hui, Lei, Zu-Ying, Liu, Xiao-Xiao, Xie, Ning, Zhong, Ling-Jing, Zhang, Min-Jie, Cao, Duanquan, Lin, Tengchuan, Jin, Changgong, Zhang, Yu-Lei, Chen   +9 more
openaire   +2 more sources

Cytoplasmic and periplasmic expression of recombinant shark VNAR antibody in Escherichia coli

Preparative Biochemistry and Biotechnology, 2019
Shark variable new antigen receptors (VNARs) are known to possess excellent heat-stability, and the long complementarity determining region 3 (CDR3) has permitted it to penetrate into the cleft region of antigens. The number of cysteine (Cys) residues contained within VNAR is greater than in conventional antibodies, entailing disulfide bond formation ...
Herng C. Leow, Katja Fischer, Yee C. Leow, Katleen Braet, Qin Cheng, James McCarthy   +5 more
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Overview and Discovery of IgNARs and Generation of VNARs

2012
Immunoglobulin new antigen receptors (IgNARs) from sharks are a distinct class of immune receptors, consisting of homodimers with no associated light chains. Antigen binding is encapsulated within single VNAR immunoglobulin domains of 13-14 kDa in size.
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Next-generation flexible formats of VNAR domains expand the drug platform's utility and developability

Biochemical Society Transactions, 2018
Therapeutic mAbs have delivered several blockbuster drugs in oncology and autoimmune inflammatory disease. Revenue for mAbs continues to rise, even in the face of competition from a growing portfolio of biosimilars. Despite this success, there are still limitations associated with the use of mAbs as therapeutic molecules.
Obinna C, Ubah, Magdalena J, Buschhaus, Laura, Ferguson, Marina, Kovaleva, John, Steven, Andrew J, Porter, Caroline J, Barelle   +6 more
openaire   +2 more sources

Design and Construction of Antibody Fusion Proteins Incorporating Variable New Antigen Receptor (VNAR) Domains

2023
Antibody therapeutics have become a cornerstone of the pharmaceutical market due to their precise molecular targeting, favorable pharmacokinetic properties, and multitiered mechanisms of action. Since the first monoclonal antibody was clinically approved 35 years ago, there have been considerable advances in antibody technology.
Seth D, Ludwig, Angela, Zhu, Ankith P, Maremanda, Helen M, Dooley, Jamie B, Spangler   +4 more
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Generation of VNAR Libraries from Immunized Sharks and Selection of Target-Specific Clones

2021
Cartilaginous fishes (sharks, skates, rays, and chimeras) are the most phylogenetically distant lineage relative to mammals in which somatically rearranging immunoglobulins (Igs or antibodies) have also been found. Alongside their conventional (heavy-light chain) isotypes, IgM and IgW, sharks produce the novel isotype, IgNAR, a heavy-chain homodimer ...
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Blood‐brain barrier transport using a high affinity, brain‐selective VNAR antibody targeting transferrin receptor 1

The FASEB Journal, 2020
Abstract Transfer across the blood‐brain barrier (BBB) remains a significant hurdle for the development of biopharmaceuticals with therapeutic effects within the central nervous system. We established a functional selection method to identify high affinity single domain antibodies to the transferrin receptor 1 (TfR1) with efficient ...
Stocki, Pawel, Szary, Jaroslaw, Rasmussen, Charlotte L M; id_orcid 0000-0001-6535-3729, Demydchuk, Mykhaylo, Northall, Leandra, Logan, Diana Bahu, Gauhar, Aziz, Thei, Laura, Moos, Torben, Walsh, Frank S, Rutkowski, J Lynn   +10 more
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In Vitro Maturation of a Humanized Shark VNAR Domain to Improve Its Biophysical Properties

2019
VNAR domains are the binding regions of new antigen receptor proteins (IgNAR) which are unique to sharks, skates, and rays (Elasmobranchii). Individual VNAR domains can bind antigens independently and are the smallest reported adaptive immune recognition entities in the vertebrate kingdom.
John, Steven, Obinna C, Ubah, Magdalena, Buschhaus, Marina, Kovaleva, Laura, Ferguson, Andrew J, Porter, Caroline J, Barelle   +6 more
openaire   +2 more sources

Characterization and humanization of VNARs targeting human serum albumin from the whitespotted bamboo shark (Chiloscyllium plagiosum)

International Journal of Biological Macromolecules
The Shark-derived immunoglobulin new antigen receptors (IgNARs) have gained increasing attention for their high solubility, exceptional thermal stability, and intricate sequence variation. In this study, we immunized whitespotted bamboo shark (Chiloscyllium plagiosum) to create phage display library of variable domains of IgNAR (VNARs) for screening ...
Weijie, Li, Mingliang, Chen, Tao, Wang, Xin, Feng, Xierui, Jiang, Xiaoning, Dong, Huan, Zhang, Xixiang, Tang, Rui, Tian, Yongyou, Zhang, Zengpeng, Li   +10 more
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Antigen specific VNAR screening in whitespotted bamboo shark (Chiloscyllium plagiosum) with next generation sequencing

Fish & Shellfish Immunology
IgNAR exhibits significant promise in the fields of cancer and anti-virus biotherapies. Notably, the variable regions of IgNAR (VNAR) possess comparable antigen binding affinity with much smaller molecular weight (∼12 kDa) compared to IgNAR. Antigen specific VNAR screening is a changeling work, which limits its application in medicine and therapy ...
Bo Zhong, Shengjie Sun, Zhan Luo, Junjie Yang, Lei Jia, Kaixi Zheng, Wenjie Tang, Xiaofeng Jiang, Zhengbing Lyu, Jianqing Chen, Guodong Chen   +10 more
openaire   +2 more sources