Protocol for high-yield bacterial expression and purification of the voltage-dependent anion channel 1 for high-throughput biophysical assays [PDF]
STAR ProtocolsSummary: Voltage-dependent anion channel 1 (VDAC1) is a key protein in cellular metabolism and apoptosis. Here, we present a protocol to express and purify milligram amounts of recombinant VDAC1 in Escherichia coli.
Stefano Conti Nibali +8 more
doaj +3 more sources
Expression profiling of mitochondrial voltage-dependent anion channel-1 associated genes predicts recurrence-free survival in human carcinomas. [PDF]
PLoS ONE, 2014 Mitochondrial voltage-dependent anion channels (VDACs) play a key role in mitochondria-mediated apoptosis. Both in vivo and in vitro evidences indicate that VDACs are actively involved in tumor progression.
Jae-Hong Ko +4 more
doaj +2 more sources
BMC Cancer, 2010 Background Activation of the extrinsic apoptosis pathway by tumour necrosis factor related apoptosis inducing ligand (TRAIL) is a novel therapeutic strategy for treating cancer that is currently under clinical evaluation.
Longley Daniel B +4 more
doaj +2 more sources
Arabidopsis voltage-dependent anion channel 1 (AtVDAC1) is required for female development and maintenance of mitochondrial functions related to energy-transaction. [PDF]
PLoS ONE, 2014 The voltage-dependent anion channels (VDACs), prominently localized in the outer mitochondrial membrane, play important roles in the metabolite exchange, energy metabolism and mitochondria-mediated apoptosis process in mammalian cells.
Xiaodi Pan +3 more
doaj +2 more sources
Cellular Physiology and Biochemistry, 2014 Background/Aims: Voltage-dependent anion channels (VDAC), also known as eukaryotic porins, are located in the outer mitochondrial membrane and allow the flux of ions and small metabolites. While the pore-forming ability of recombinant VDAC1 and VDAC2 has
Vanessa Checchetto +4 more
doaj +2 more sources
VDAC1 protein derived from extracellular vesicles promotes paclitaxel resistance in gastric cancer through autophagy and mitophagy [PDF]
Cancer Biology & MedicineObjective: Paclitaxel (PTX), a conventional second-line therapeutic agent for advanced gastric cancer (GC), exhibits compromised clinical efficacy due to acquired chemoresistance in patients, the molecular mechanisms of which remain poorly elucidated ...
Yanna Bi +10 more
doaj +2 more sources
Efficient PHB2 (prohibitin 2) exposure during mitophagy depends on VDAC1 (voltage dependent anion channel 1). [PDF]
AutophagyExposure of inner mitochondrial membrane resident protein PHB2 (prohibitin 2) during autophagic removal of depolarized mitochondria (mitophagy) depends on the ubiquitin-proteasome system. This uncovering facilitates the PHB2 interaction with phagophore
Roy M +7 more
europepmc +2 more sources
Multiple neurosteroid and cholesterol binding sites in voltage-dependent anion channel-1 determined by photo-affinity labeling. [PDF]
Biochim Biophys Acta Mol Cell Biol Lipids, 2019 Cheng WWL +12 more
europepmc +2 more sources
The Voltage-dependent Anion Channel 1 Mediates Amyloid β Toxicity and Represents a Potential Target for Alzheimer Disease Therapy. [PDF]
J Biol Chem, 2015 Smilansky A +5 more
europepmc +2 more sources
Lidocaine promotes apoptosis in breast cancer cells by affecting VDAC1 expression
BMC Anesthesiology, 2022 Objective To investigate the effect of lidocaine on the expression of voltage-dependent anion channel 1 (VDAC1) in breast invasive carcinoma (BRCA) and its impact on the apoptosis of breast cancer cells.
Dingde Long +5 more
doaj +1 more source