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Identification and implication of an allergenic PR‐10 protein from walnut in birch pollen associated walnut allergy

Molecular Nutrition & Food Research, 2017
ScopeEnglish walnut (Juglans regia) belongs to the most important allergenic tree nuts. Co‐sensitization with birch (Betula verrucosa) pollen has been reported. We aimed to identify a walnut allergen homologous to the major birch pollen allergen Bet v 1.Methods and resultsA cDNA encoding a Bet v 1‐homologous allergen (Jug r 5) in walnut kernels was ...
Wangorsch, Andrea   +7 more
openaire   +3 more sources

Solubilization mechanism of self‐assembled walnut protein nanoparticles and curcumin encapsulation

Journal of the Science of Food and Agriculture, 2023
AbstractBACKGROUNDNative walnut protein is an alkali‐soluble protein that seriously limits the application of walnut protein. The pH‐shifting method could improve the solubility of walnut proteins and enable the encapsulation of active ingredients.
Jiao Lv   +4 more
openaire   +2 more sources

Enzymolysis Optimization of Walnut Residue Protein Using Trypsase

Advanced Materials Research, 2013
The enzymolysis of walnut residue protein was investigated using trypsase, and the best process conditions were studied by one-factor experiment and orthogonal experiment. The result showed that the influential degree weakened as follows sequence: temperature, pH, enzyme quantity, and time.
Shao Ying Zhang, Yin Zhe Ren
openaire   +1 more source

Raw walnut kernel: A natural source for dietary proteases and bioactive proteins

Food Chemistry, 2022
Walnut kernels are health-promoting nuts, which are mainly attributed to polyunsaturated fatty acids, phenolics, and phytosterols. However, the information concerning benefits of walnut proteins are limited. In this study, endopeptidases, aminopeptidases, carboxypeptidases, superoxide dismutases, catalases, and phospholipases with respective relative ...
Yeming, Chen   +5 more
openaire   +2 more sources

Identification of Key Lipogenesis Stages and Proteins Involved in Walnut Kernel Development

Journal of Agricultural and Food Chemistry, 2023
Walnuts are abundant in oil content, especially for polyunsaturated fatty acids, but the understanding of their formation is limited. We collected walnut (Juglans regia L.) kernels at 60, 74, 88, 102, 116, 130, and 144 days after pollination (designated S1-S7).
Feng Jin   +8 more
openaire   +2 more sources

Surface properties of walnut protein from AOT reverse micelles

International Journal of Food Science & Technology, 2013
SummaryThe surface chemical composition and microstructure of walnut protein obtained through aqueous buffer and bis(2‐ethylhexyl) sodium sulfosuccinate (AOT) reverse micelles were determined by X‐ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM). The surfaces were characterised by XPS to monitor surface composition.
Fenglan Liu   +5 more
openaire   +1 more source

Purification and Identification of an ACE Inhibitory Peptide from Walnut Protein

Journal of Agricultural and Food Chemistry, 2013
In the present study, a novel angiotensin I-converting enzyme (ACE)-inhibitory peptide, P-2a2, was purified to homogeneity from walnut protein hydrolysate by ultrafiltration, consecutive column chromatography, and high-performance liquid chromatography. The purified peptide was characterized by matrix-assisted laser desorption ionization time-of-flight
Meng, Liu   +6 more
openaire   +2 more sources

Quantitative indirect ELISA for determination of walnut proteins in foods

Food Science and Biotechnology, 2015
An indirect enzyme-linked immunosorbent assay (ELISA) capable of detecting walnut proteins in commercial products was developed. Polyclonal antibodies against soluble walnut protein were used. Specific epitopes of walnut protein were analyzed for cross activity with peanut, soybean, sesame, rice, wheat, jujube, and defatted milk protein.
Juan Fang   +6 more
openaire   +1 more source

Development of an indirect competitive immunoassay for walnut protein component in food

Food Chemistry, 2014
Among food allergens, walnut is a frequent cause of adverse food reactions in allergic patients. In this study, the walnut allergen protein 2S albumin precursor (Jug r 1) cDNA was synthesised and cloned into the pGEX-6P-1 expression vector. The recombinant plasmids were transformed into Escherichia coli (E.
Haiyan, Wang   +4 more
openaire   +2 more sources

Enhancing functionality and bioactivity of walnut protein through limited enzyme digestion

Journal of the Science of Food and Agriculture
AbstractBACKGROUNDWalnut protein (WP) is recognized as a valuable plant protein. However, the poor solubility and functional properties limit its application in the food industry. It is a great requirement to improve the physicochemical properties of WP.RESULTSFollowing a 90 min restricted enzymatic hydrolysis period, the solubility of WP significantly
Jinping Wang   +4 more
openaire   +2 more sources

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