Results 41 to 50 of about 1,661 (174)

Igh and Igk loci use different folding principles for V gene recombination due to distinct chromosomal architectures of pro-B and pre-B cells

Nature Communications, 2023
Extended loop extrusion across the immunoglobulin heavy-chain (Igh) locus facilitates VH-DJH recombination following downregulation of the cohesin-release factor Wapl by Pax5, resulting in global changes in the chromosomal architecture of pro-B cells ...
Louisa Hill, Gordana Wutz, Markus Jaritz, Hiromi Tagoh, Lesly Calderón, Jan-Michael Peters, Anton Goloborodko, Meinrad Busslinger   +7 more
doaj   +1 more source

What AlphaFold tells us about cohesin’s retention on and release from chromosomes

eLife, 2023
Cohesin is a trimeric complex containing a pair of SMC proteins (Smc1 and Smc3) whose ATPase domains at the end of long coiled coils (CC) are interconnected by Scc1.
Kim A Nasmyth, Byung-Gil Lee, Maurici Brunet Roig, Jan Löwe   +3 more
doaj   +1 more source

Co-depletion of NIPBL and WAPL restores TAD boundary strength by Oligopaint FISH.

, 2022
(A) Fluorescent western blot to NIPBL (top band, see arrow) and WAPL in nuclear (nuc) and chromatin-bound (chr) subcellular protein fractionations of RNAi control, NIPBL, WAPL, and double knockdown (dKD) depleted HCT116 cells. All bands are from the same
Rebecca Yunker (14197613), Parisha P. Shah (9380961), Eric F. Joyce (7900052), Rajan Jain (422776), Karen Adelman (4540336), Jennifer M. Luppino (14197610), Yemin Lan (330697), Andrew Field (12393694), Son C. Nguyen (1332762), Richard J. Abdill (6719147), Daniel S. Park (14117041)   +10 more
core   +1 more source

The Role of Structural Maintenance of Chromosomes Complexes in Meiosis and Genome Maintenance: Translating Biomedical and Model Plant Research Into Crop Breeding Opportunities

Frontiers in Plant Science, 2021
Cohesin is a multi-unit protein complex from the structural maintenance of chromosomes (SMC) family, required for holding sister chromatids together during mitosis and meiosis.
Pablo Bolaños-Villegas, Pablo Bolaños-Villegas   +1 more
doaj   +1 more source

MCPH1 inhibits Condensin II during interphase by regulating its SMC2-Kleisin interface

eLife, 2021
Dramatic change in chromosomal DNA morphology between interphase and mitosis is a defining features of the eukaryotic cell cycle. Two types of enzymes, namely cohesin and condensin confer the topology of chromosomal DNA by extruding DNA loops.
Martin Houlard, Erin E Cutts, Muhammad S Shamim, Jonathan Godwin, David Weisz, Aviva Presser Aiden, Erez Lieberman Aiden, Lothar Schermelleh, Alessandro Vannini, Kim Nasmyth   +9 more
doaj   +1 more source

Correction of water vapor absorption for aerosol remote sensing with ceilometers [PDF]

Atmospheric Measurement Techniques, 2015
In recent years attention was increasingly paid to backscatter profiles of ceilometers as a new source of aerosol information. Several case studies have shown that – although originally intended for cloud detection only – ceilometers can provide the ...
M. Wiegner, J. Gasteiger
doaj   +1 more source

Human Wapl Is a Cohesin-Binding Protein that Promotes Sister-Chromatid Resolution in Mitotic Prophase [PDF]

, 2006
BACKGROUND: The linkage between duplicated chromosomes (sister chromatids) is established during S phase by the action of cohesin, a multisubunit complex conserved from yeast to humans.
Rita Gandhi, Tatsuya Hirano, Gillespie, Peter J., Peter J. Gillespie, Gillespie, P. J., Gandhi, Rita, Gandhi, R., Hirano, Tatsuya, Hirano, T.   +8 more
core   +1 more source

ESCO1 and CTCF enable formation of long chromatin loops by protecting cohesinSTAG1 from WAPL

eLife, 2020
Eukaryotic genomes are folded into loops. It is thought that these are formed by cohesin complexes via extrusion, either until loop expansion is arrested by CTCF or until cohesin is removed from DNA by WAPL.
Gordana Wutz, Rene Ladurner, Brian Glenn St Hilaire, Roman R Stocsits, Kota Nagasaka, Benoit Pignard, Adrian Sanborn, Wen Tang, Csilla Várnai, Miroslav P Ivanov, Stefan Schoenfelder, Petra van der Lelij, Xingfan Huang, Gerhard Dürnberger, Elisabeth Roitinger, Karl Mechtler, Iain Finley Davidson, Peter Fraser, Erez Lieberman-Aiden, Jan-Michael Peters   +19 more
doaj   +1 more source

Eco1-dependent cohesin acetylation anchors chromatin loops and cohesion to define functional meiotic chromosome domains

eLife, 2022
Cohesin organizes the genome by forming intra-chromosomal loops and inter-sister chromatid linkages. During gamete formation by meiosis, chromosomes are reshaped to support crossover recombination and two consecutive rounds of chromosome segregation ...
Rachael E Barton, Lucia F Massari, Daniel Robertson, Adèle L Marston   +3 more
doaj   +1 more source

K29-Linked Ubiquitination of Transcription Regulators Controls Cell Proliferation in the Unfolded Protein Response. [PDF]

Adv Sci (Weinh)
Under endoplasmic reticulum stress, cells activate the unfolded protein response (UPR). During the UPR, the cohesin complex at the promoter of cell proliferation‐related genes undergoes K29‐linked ubiquitination, recruiting the cohesin release factor WAPL to release cohesin, leading to transcriptional downregulation and inhibition of cell growth ...
Zhang Q, Teng X, Dai Y, Wu Y, Hou H, Li J.   +5 more
europepmc   +2 more sources