Results 51 to 60 of about 1,554 (181)

Wapl mutants exhibit chromosome stretching during anaphase.

, 2014
(A) Live-imaging of chromosomes in wild-type and waplC204 neuroblast cells. Time 0∶00 equals anaphase onset and scale bars is 10 µm. (B) Quantification of anaphase chromosome length in wild-type and waplC204.
William Sullivan (37486), Christian S. Eichinger (641028), Alexandra Tavares (144746), Raquel A. Oliveira (641024), Mihailo Mirkovic (641026), Shaila Kotadia (641025), Katherine Bowlin (641027), Kim Nasmyth (71271)   +7 more
core   +1 more source

WAPL maintains a cohesin loading cycle to preserve cell-type-specific distal gene regulation [PDF]

, 2021
The cohesin complex has an essential role in maintaining genome organization. However, its role in gene regulation remains largely unresolved. Here we report that the cohesin release factor WAPL creates a pool of free cohesin, in a process known as ...
Elphѐge P. Nora, Liu, Ning Qing, de Wit, Elzo, Teun van den Brand, Luca Braccioli, Teunissen, Hans, Marijne M. G. A. Schijns, Elzo de Wit, Ning Qing Liu, Michela Maresca, Bruneau, Benoit G, Maresca, Michela, Nora, Elphѐge P, Hans Teunissen, Schijns, Marijne MGA, Benoit G. Bruneau, van den Brand, Teun, Braccioli, Luca   +17 more
core   +1 more source

Replication factor C complexes play unique pro- and anti-establishment roles in sister chromatid cohesion. [PDF]

PLoS ONE, 2010
Recent studies have lead to a rapid expansion of sister chromatid cohesion pathways. Of particular interest is the growth in classifications of anti-establishment factors-now including those that are cohesin-associated (Rad61/WAPL and Pds5) or DNA ...
Marie E Maradeo, Robert V Skibbens
doaj   +1 more source

Interaction Between Rec8 and Mis4 Is Required for Axis‐Loop Chromatin Formation and Homologous Chromosome Recombination During Meiosis

Genes to Cells, Volume 31, Issue 4, July 2026.
We identified a Mis4 cohesin loader mutant that is specifically defective in chromatin axis formation and homologous recombination while retaining normal sister chromatid cohesion. The mis4‐LR mutant, carrying the L1150S and R1159G amino acid substitutions, exhibited reduced interaction between Mis4 and Rec8, suggesting a meiosis‐specific role for the ...
Takeshi Sakuno, Yasuto Murayama, Tokuko Haraguchi, Yasushi Hiraoka   +3 more
wiley   +1 more source

Partial depletion of NIPBL or WAPL separate the role of cohesin in chromatin folding from chromosome segregation.

, 2022
(A) Cartoon depicting the roles of the two opposing cohesin regulators; NIPBL loads cohesin onto chromatin and is required for loop extrusion whereas WAPL opens the ring and removes it. (B) Fluorescent western blot to NIPBL (top band, see arrow) and WAPL
Rebecca Yunker (14197613), Parisha P. Shah (9380961), Eric F. Joyce (7900052), Rajan Jain (422776), Karen Adelman (4540336), Jennifer M. Luppino (14197610), Yemin Lan (330697), Andrew Field (12393694), Son C. Nguyen (1332762), Richard J. Abdill (6719147), Daniel S. Park (14117041)   +10 more
core   +1 more source

Protein turnover downstream of the Nipbl/CRL4 axis contributes to abnormal development in zebrafish embryos

Developmental Dynamics, Volume 255, Issue 4, Page 403-416, April 2026.
Abstract Background Mutations in cohesins cause cohesinopathies such as Cornelia de Lange Syndrome (CdLS) and Roberts Syndrome (RBS). Prior findings demonstrate that Esco2 (a cohesin activator) and Smc3 (a core cohesin subunit) regulate the CRL4 E3 ubiquitin ligase. SMC3 mutations, however, account for a small percentage of CdLS.
Annie C. Sanchez, Niusha Banoukh, Fiona Mensching, Robert V. Skibbens, M. Kathryn Iovine   +4 more
wiley   +1 more source

HP1 Promotes the Centromeric Localization of ATRX and Protects Cohesion by Interfering Wapl Activity in Mitosis

Frontiers in Bioscience-Landmark
Background: α thalassemia/mental retardation syndrome X-linked (ATRX) serves as a part of the sucrose nonfermenting 2 (SNF2) chromatin-remodeling complex.
Erchen Zhang, Lei Peng, Kejia Yuan, Zexian Ding, Qi Yi   +4 more
doaj   +1 more source

Pds5A and Pds5B Display Non-redundant Functions in Mitosis and Their Loss Triggers Chk1 Activation

Frontiers in Cell and Developmental Biology, 2020
BackgroundPds5 is an abundant HEAT-repeat-containing protein that binds to cohesin and mediates sister chromatid cohesion. In vertebrates, Pds5A and Pds5B are known to protect DNA replication fork, as their loss leads to DNA damage.
Naif Al-Jomah, Naif Al-Jomah, Lubinda Mukololo, Lubinda Mukololo, Awais Anjum, Awais Anjum, Mohammed Al Madadha, Mohammed Al Madadha, Raj Patel   +8 more
doaj   +1 more source

Molecular characterisation of human penile carcinoma and generation of paired epithelial primary cell lines

Molecular Oncology, Volume 20, Issue 2, Page 260-281, February 2026.
Generation of two normal and tumour (cancerous) paired human cell lines using an established tissue culture technique and their characterisation is described. Cell lines were characterised at cellular, protein, chromosome and gene expression levels and for HPV status.
Simon Broad, Mahmood Hachim, Tom Bertin, Karolina Penderecka, Rifat Hamoudi, Saif Khan, Rui Henrique, Natalie Bergmoser, Manit Arya, Kalle Sipila, Matteo Vietri Rudan, Asif Muneer, Aamir Ahmed   +12 more
wiley   +1 more source

Enhancer–promoter interactions and transcription are largely maintained upon acute loss of CTCF, cohesin, WAPL or YY1 [PDF]

, 2022
It remains unclear why acute depletion of CTCF (CCCTC-binding factor) and cohesin only marginally affects expression of most genes despite substantially perturbing three-dimensional (3D) genome folding at the level of domains and structural loops.
Hansen, Anders S, Cattoglio, Claudia, Slobodyanyuk, Elena, Hsieh, Tsung-Han S, Hansen, Anders S., Darzacq, Xavier, Tjian, Robert, Hsieh, Tsung-Han S.   +7 more
core   +1 more source