Results 131 to 140 of about 3,060 (171)

The Activity of Xylose Reductase and Xylitol Dehydrogenase in Yeasts

Microbiology, 2003
The activity and the cofactor specificity of xylose reductase and xylitol dehydrogenase were studied in extracts of yeasts from the genera Candida, Kluyveromyces, Pachysolen, Pichia, and Torulopsis grown under microaerobic conditions. It was found that xylitol dehydrogenase in all of the yeast species studied is specific for NAD+; xylose reductase in ...
E N, Iablochkova, O I, Bolotnikova, N P, Mikhaĭlova, N N, Nemova, A I, Ginak   +4 more
openaire   +2 more sources

Enhancement of xylitol production by attenuation of intracellular xylitol dehydrogenase activity in Candida tropicalis

Biotechnology Letters, 2011
To construct Candida tropicalis strains that produce a high yield of xylitol with no requirement for co-substrates, we engineered the yeast with an attenuated xylitol dehydrogenase (XDH) and then assessed the efficiency of xylitol production The mutants, strains XDH-5 (with only one copy of the XDH gene), and ARSdR-16 (with a mutated XDH gene) showed ...
Ko, BS Ko, Byoung-Sam, Kim, DM Kim, Dong-Min, Yoon, BH Yoon, Byoung-Hoon, Bai, S Bai, Suk, Lee, HY Lee, Hyeon-Yong, Kim, JH Kim, Jung-Hoe, Kim, IC Kim, Il-Chul   +6 more
openaire   +2 more sources

NAD+-dependent xylitol dehydrogenase (xdhA) and l-arabitol-4-dehydrogenase (ladA) deletion mutants of Aspergillus oryzae for improved xylitol production

Biotechnology Letters, 2013
Xylitol dehydrogenase (XDHA) and L-arabitol dehydrogenase (LADA) are two key enzymes in xylan metabolism catalyzing the oxidation of xylitol to D-xylulose and arabitol to L-xylulose, respectively. In Aspergillus oryzae, XDHA and LADA are encoded by xdhA and ladA.
Noriyuki Kitamoto, Kazuhiro Takamizawa
exaly   +3 more sources

Utilization of Xylitol Dehydrogenase in a Combined Microbial/Enzymatic Process for Production of Xylitol from D-lucose

Applied Biochemistry and Biotechnology, 2002
The production of xylitol from D-glucose occurs through a three-step process in which D-arabitol and D-xylulose are formed as the first and second intermediate product, respectively, and both are obtained via microbial bioconversion reactions. Catalytic hydrogenation of D-xylulose yields xylitol; however, it is contaminated with D-arabitol.
Gerhard, Mayer, Klaus D, Kulbe, Bernd, Nidetzky   +2 more
openaire   +2 more sources

Identification of a Xylitol Dehydrogenase Gene from Kluyveromyces marxianus NBRC1777

Molecular Biotechnology, 2012
Xylitol dehydrogenase (XDH) (EC 1.1.1.9) is one of the key enzymes in the xylose fermentation pathway in yeast and fungi. A xylitol dehydrogenase gene (XYL2) encoding a XDH was cloned from Kluyveromyces marxianus NBRC 1777, and the in vivo function was validated by disruption and complementation analysis. The highest activity of KmXDH could be observed
Li, Lulu, Zhang, Ling, Wang, Dongmei, Gao, Xiaolian, Tamaki, Hisanori, Kumagai, Hidehiko, Hong, Jiong   +6 more
openaire   +2 more sources

Purification and characterisation of xylitol dehydrogenase from Neurospora crassa

FEMS Microbiology Letters, 1997
The purification and characterisation of one of the key enzymes in the conversion of xylose to ethanol, xylitol dehydrogenase (XD), from Neurospora crassa is reported. This organism has the unique ability to directly convert biomass to ethanol. The enzyme was purified 81-fold by fractional ammonium sulfate precipitation, column chromatography on DEAE ...
exaly   +2 more sources

Construction of recombinant Escherichia coli expressing xylitol-4-dehydrogenase and optimization for enhanced L-xylulose biotransformation from xylitol

Bioprocess and Biosystems Engineering, 2021
L-Xylulose is a rare ketopentose which inhibits α-glucosidase and is an indicator of hepatitis or liver cirrhosis. This pentose is also a precursor of other rare sugars such as L-xylose, L-ribose or L-lyxose. Recombinant E. coli expressing xylitol-4-dehydrogenase gene of Pantoea ananatis was constructed.
Mesfin Angaw, Tesfay, Xin, Wen, Yujie, Liu, Huibin, Lin, Linxu, Chen, Jianqiang, Lin, Jianqun, Lin   +6 more
openaire   +2 more sources

Isolation and characterization of two xylitol dehydrogenases from Aspergillus niger

Microbiology, 1994
An NADPH-dependent L-xylulose reductase [xylitol:NADP 4-oxidoreductase; EC 1.1.1.10 (LXDH)] from Aspergillus niger was purified and characterized. It is an octamer with a monomeric molecular mass of 32 kDa, showing high specificity for L-xylulose, xylitol and NADP(H).
Witteveen, C.B.F., Weber, F., Busink, R., Visser, J.   +3 more
openaire   +1 more source

Purification of Xylitol Dehydrogenase and Improved Production of Xylitol by Increasing XDH Activity and NADH Supply in Gluconobacter oxydans

Journal of Agricultural and Food Chemistry, 2013
Gluconobacter oxydans is known to be a suitable candidate for producing xylitol from d-arabitol. In this study, the enzyme responsible for reducing d-xylulose to xylitol was purified from G. oxydans NH-10 and characterized as xylitol dehydrogenase.
Jinliang, Zhang, Sha, Li, Hong, Xu, Peng, Zhou, Lujia, Zhang, Pingkai, Ouyang   +5 more
openaire   +2 more sources

Induction of the xylitol dehydrogenase of Pullularia pullulans

Canadian Journal of Microbiology, 1988
Pullularia pullulans, a yeastlike fungus, metabolizes xylitol via an intracellular xylitol dehydrogenase. Formation of the enzyme was induced by D-xylose and inhibited by cycloheximide, suggesting de novo synthesis. The induction study was carried out with noninduced cells of P. pullulans harvested from a glycerol medium. The cells initiated synthesis
Juliet K. Sugai, L. A. Veiga
openaire   +1 more source