Comparing the xylose reductase/xylitol dehydrogenase and xylose isomerase pathways in arabinose and xylose fermenting
Biotechnology for Biofuels, 2008 Background Ethanolic fermentation of lignocellulosic biomass is a sustainable option for the production of bioethanol. This process would greatly benefit from recombinant Saccharomyces cerevisiae strains also able to ferment, besides the hexose sugar ...
Hahn-Hägerdal Bärbel +2 more
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Comparison of the xylose reductase-xylitol dehydrogenase and the xylose isomerase pathways for xylose fermentation by recombinant
Microbial Cell Factories, 2007 Background Two heterologous pathways have been used to construct recombinant xylose-fermenting Saccharomyces cerevisiae strains: i) the xylose reductase (XR) and xylitol dehydrogenase (XDH) pathway and ii) the xylose isomerase (XI) pathway.
Hahn-Hägerdal Bärbel +3 more
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Fermentation of mixed glucose-xylose substrates by engineered strains of
Microbial Cell Factories, 2010 Background In spite of the substantial metabolic engineering effort previously devoted to the development of Saccharomyces cerevisiae strains capable of fermenting both the hexose and pentose sugars present in lignocellulose hydrolysates, the ...
Klimacek Mario +5 more
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Structural insight into D-xylose utilization by xylose reductase from Scheffersomyces stipitis. [PDF]
Sci Rep, 2018 AbstractLignocellulosic biomass, of which D-xylose accounts for approximately 35% of the total sugar, has attracted attention as a future energy source for biofuel. To elucidate molecular mechanism of D-xylose utilization, we determined the crystal structure of D-xylose reductase from Schefferzomyces stipitis (SsXR) at a 1.95 Å resolution.
Son HF, Lee SM, Kim KJ.
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Synthesis of Amines for Active Pharmaceutical Ingredients Using the Whole-Cell Factory Saccharomyces Cerevisae. [PDF]
ChembiochemThis review highlights advances in engineering Saccharomyces cerevisiae for the sustainable biocatalytic production of pharmaceutically relevant amines, amino alcohols, amino acids, and complex alkaloids. It focuses on strategies to increase productivity, including enhanced enzyme expression, cofactor regeneration, precursor channeling, pathway ...
Kwiatos N, Ossel S, Mutti FG.
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The Weimberg pathway: an alternative for Myceliophthora thermophila to utilize d-xylose
Biotechnology for Biofuels and Bioproducts, 2023 Background With d-xylose being the second most abundant sugar in nature, its conversion into products could significantly improve biomass-based process economy. There are two well-studied phosphorylative pathways for d-xylose metabolism. One is isomerase
Defei Liu +5 more
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Genetics and Molecular Biology, 2011 Two novel endophytic yeast strains, WP1 and PTD3, isolated from within the stems of poplar (Populus) trees, were genetically characterized with respect to their xylose metabolism genes. These two strains, belonging to the species Rhodotorula graminis and
Ping Xu, Renata Bura, Sharon L. Doty
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Biotechnology for Biofuels, 2008 Background Pichia stipitis xylose reductase (Ps-XR) has been used to design Saccharomyces cerevisiae strains that are able to ferment xylose. One example is the industrial S. cerevisiae xylose-consuming strain TMB3400, which was constructed by expression
Röder Anja +4 more
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Biotechnology for Biofuels and Bioproducts, 2023 Background Over the last decades, many strategies to procure and improve xylose consumption in Saccharomyces cerevisiae have been reported. This includes the introduction of efficient xylose-assimilating enzymes, the improvement of xylose transport, or ...
Viktor C. Persson +4 more
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Balance of XYL1 and XYL2 expression in different yeast chassis for improved xylose fermentation
Frontiers in Microbiology, 2012 Reducing xylitol formation is an important step in engineering xylose reductase/xylitol dehydrogenase pathway for ethanol production from xylose in Saccharomyces cerevisiae. In this study, to balance the expression of XYL1 and mutant XYL2 encoding xylose
Jian eZha +5 more
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