Binding pocket stabilization by high-throughput screening of yeast display libraries [PDF]
Frontiers in Molecular Biosciences, 2022 Protein dynamics have a great influence on the binding pockets of some therapeutic targets. Flexible protein binding sites can result in transient binding pocket formation which might have a negative impact on drug screening efforts.
Jorge A. Lerma Romero +8 more
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Native llama Nanobody Library Panning Performed by Phage and Yeast Display Provides Binders Suitable for C-Reactive Protein Detection [PDF]
Biosensors, 2021 C-reactive protein (CRP) is an inflammation biomarker that should be quantified accurately during infections and healing processes. Nanobodies are good candidates to replace conventional antibodies in immunodiagnostics due to their inexpensive production,
Sandra Oloketuyi +9 more
doaj +2 more sources
Yeast Display Guided Selection of pH-Dependent Binders. [PDF]
Methods Mol Biol, 2022 pH-dependent antigen binding has proven useful in engineering next-generation therapeutics specifically via antibody recycling technology. This technology allows for half-life extension, thereby lowering the amount and frequency of dosing of therapeutics. Cell sorting, coupled with display techniques, has been used extensively for the selection of high-
Meanor JN, Keung AJ, Rao BM, Gera N.
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A switchable yeast display/secretion system. [PDF]
Protein Eng Des Sel, 2015 Display technologies such as yeast and phage display offer powerful alternatives to traditional immunization-based antibody discovery, but require conversion of displayed proteins into soluble form prior to downstream characterization. Here we utilize amber suppression to implement a yeast-based switchable display/secretion system that enables the ...
Van Deventer JA +4 more
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Synthetic integrin antibodies discovered by yeast display reveal αV subunit pairing preferences with β subunits [PDF]
mAbsIntegrins are cell surface receptors that mediate the interactions of cells with their surroundings and play essential roles in cell adhesion, migration, and homeostasis.
Yuxin Hao +11 more
doaj +2 more sources
Isolation of a Monoclonal Human scFv Against Cytomegalovirus pp71 Antigen Using Yeast Display [PDF]
AntibodiesBackground: Human cytomegalovirus (CMV) is a major pathogen that poses significant risks to immunocompromised individuals and neonates. The tegument protein pp71, encoded by the UL82 gene, plays a pivotal role in initiating viral lytic replication and ...
Kazuhisa Aoki +5 more
doaj +2 more sources
Isolation of HCV Neutralizing Antibodies by Yeast Display. [PDF]
Methods Mol Biol, 2019 Yeast surface display (YSD) enables efficient screening and selection of single chain variable fragments (scFvs) of heavy (VH) and light (VL) chains that bind to target antigen with different affinities. Assembly of a scFv library from cDNA usually involves adding different primers and linkers (Gly4/Ser)3 through multiple rounds of PCR amplification ...
Keck ZY, Wang Y, Lau P, Foung SKH.
europepmc +4 more sources
Integration of phage and yeast display platforms: A reliable and cost effective approach for binning of peptides as displayed on-phage. [PDF]
PLoS ONE, 2020 Hundreds of target specific peptides are routinely discovered by peptide display platforms. However, due to the high cost of peptide synthesis only a limited number of peptides are chemically made for further analysis.
Priyanka Pandya +16 more
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Expeditious Generation of Biparatopic Common Light Chain Antibodies via Chicken Immunization and Yeast Display Screening [PDF]
Frontiers in Immunology, 2020 Bispecific (BsAb) and biparatopic (BpAb) antibodies emerged as promising formats for therapeutic biologics exhibiting tailor-made functional properties.
Jan P. Bogen +8 more
doaj +2 more sources
Optimized single-cell gates for yeast display screening. [PDF]
Protein Eng Des SelAbstract Yeast display is a widely used technology in antibody discovery and protein engineering. The cell size of yeast enables fluorescence-activated cell sorting (FACS) to precisely screen gene libraries, including for multi-parameter selection of protein phenotypes. However, yeast cells show a broader size distribution than mammalian
Pan X +8 more
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