Results 11 to 20 of about 257,444 (202)
Susceptibility of broad reactivity nanobodies to resistance mutations in the S2 domain of SARS-CoV-2 predicted by yeast display deep mutational scanning [PDF]
Frontiers in ImmunologyIntroductionThe rapid evolution of SARS-CoV-2 has led to the erosion of vaccine induced serum neutralization and monoclonal antibody efficacy. As such, interest is inevitably moving towards more conserved regions of the SARS-CoV-2 spike protein like the ...
Christina R. Ball +3 more
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Efficient yeast surface-display of novel complex synthetic cellulosomes [PDF]
Microbial Cell Factories, 2018 Background The self-assembly of cellulosomes on the surface of yeast is a promising strategy for consolidated bioprocessing to convert cellulose into ethanol in one step.
Hongting Tang +6 more
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Mapping monoclonal anti-SARS-CoV-2 antibody repertoires against diverse coronavirus antigens
Frontiers in Immunology, 2022 Variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have emerged continuously, challenging the effectiveness of vaccines, diagnostics, and treatments.
Matheus Oliveira de Souza +20 more
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Functional Profiling of Antibody Immune Repertoires in Convalescent Zika Virus Disease Patients
Frontiers in Immunology, 2021 The re-emergence of Zika virus (ZIKV) caused widespread infections that were linked to Guillain-Barré syndrome in adults and congenital malformation in fetuses, and epidemiological data suggest that ZIKV infection can induce protective antibody responses.
Ahmed S. Fahad +21 more
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A Generic Strategy to Generate Bifunctional Two-in-One Antibodies by Chicken Immunization
Frontiers in Immunology, 2022 Various formats of bispecific antibodies exist, among them Two-in-One antibodies in which each Fab arm can bind to two different antigens. Their IgG-like architecture accounts for low immunogenicity and also circumvents laborious engineering and ...
Julia Harwardt +10 more
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Cell Reports, 2021 Summary: Understanding mechanisms of protective antibody recognition can inform vaccine and therapeutic strategies against SARS-CoV-2. We report a monoclonal antibody, 910-30, targeting the SARS-CoV-2 receptor-binding site for ACE2 as a member of a ...
Bailey B. Banach +33 more
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Evaluation of the yeast surface display system for screening of functional nanobodies
AMB Express, 2020 Yeast surface display is a powerful technology used to isolate and engineer proteins to improve their activity, specificity, and stability. In this method, gene expression is regulated by promoters, and secretion efficiency is affected by secretion ...
Kaho Kajiwara +2 more
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Frontiers in Bioengineering and Biotechnology, 2020 Affinity chromatography provides an excellent platform for protein purification, which is a key step in the large scale downstream processing of therapeutic monoclonal antibodies (Mabs).
Steffen C. Hinz +10 more
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Journal of Extracellular Vesicles, 2021 The research of extracellular vesicles (EVs) has boomed in the last decade, with the promise of them functioning as target‐directed drug delivery vehicles, able to modulate proliferation, migration, differentiation, and other properties of the recipient ...
Stefan Vogt +7 more
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The Use of Yeast in Biosensing
Microorganisms, 2022 Yeast has been used as a model for several diseases as it is the simplest unicellular eukaryote, safe and easy to culture and harbors most of the fundamental processes that are present in almost all higher eukaryotes, including humans. From understanding
Sudip Dhakal, Ian Macreadie
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