Results 31 to 40 of about 143,975 (276)

Experimental conditions may affect reproducibility of the beta-galactosidase assay [PDF]

FEMS Microbiology Letters, 1992
Several experimental conditions and parameters contributing to the determination of beta-galactosidase activity, as proposed in Miller's assay, were studied. Use of the absorbance correction factor and the nature and concentration of permeabilizing agents were taken into account as different experimental conditions.
GIACOMINI, ALESSIO, CORICH, VIVIANA, F. J. Ollero, SQUARTINI, ANDREA, M. P. Nuti   +4 more
openaire   +2 more sources

A data comparison between a traditional and the single-step β-galactosidase assay

Data in Brief, 2016
This article describes reproducibility of a single-step automated β-galactosidase, and the equivalence of its data to the traditional assay (“Experiments in Molecular Genetics” [1]).
Jorrit Schaefer, Goran Jovanovic, Ioly Kotta-Loizou, Martin Buck   +3 more
doaj   +1 more source

Cloning of the phospho-β-galactosidase gene inEscherichia colifrom lactose-negative mutants ofStreptococcus mutansisolated following random mutagenesis with plasmid pVA891 clone banks [PDF]

FEMS Microbiology Letters, 1992
In order to mutagenize Streptococcus mutans a marker rescue plasmid, pVA891, was employed. The plasmid was ligated with Sau3AI digested chromosomal DNA fragments from S. mutans GS-5IS3 and the resultant plasmids were amplified in Escherichia coli.
Yutaka Sato, Yasuhito Yamamoto, Harutoshi Kizaki, Howard K. Kuramitsu   +3 more
openaire   +1 more source

The effect of the electric field on lag phase, β-galactosidase production and plasmid stability of a recombinant Saccharomyces cerevisiae strain growing on lactose [PDF]

, 2012
Ethanol and β-galactosidase production from cheese whey may significantly contribute to minimise environmental problems while producing value from lowcost raw materials.
AB Futcher, AH Baysal, António A. Vicente, BA Rowley, C Oliveira, Carla Oliveira, DL Ludwig, GL Miller, H Yildiz, HX Sun, HY Cho, I Castro, Inês Castro, José A. Teixeira, L Domingues, L Domingues, L Domingues, L Domingues, L Loghavi, L Loghavi, L Loghavi, LF Machado, Lucília Domingues, M Stacey, MJ Bailey, OQF Araújo, P Wei, PN Bartlett, PS Panesar, R Pereira, RN Pereira, V Kumar   +31 more
core   +1 more source

Characterization and molecular cloning of a heterodimeric β-galactosidase from the probiotic strainLactobacillus acidophilusR22 [PDF]

FEMS Microbiology Letters, 2007
Beta-galactosidase from the probiotic strain Lactobacillus acidophilus R22 was purified to apparent homogeneity by ammonium sulphate fractionation, hydrophobic interaction, and affinity chromatography. The enzyme is a heterodimer consisting of two subunits of 35 and 72 kDa, as determined by gel electrophoresis.
Thu-Ha, Nguyen, Barbara, Splechtna, Stanimira, Krasteva, Wolfgang, Kneifel, Klaus D, Kulbe, Christina, Divne, Dietmar, Haltrich   +6 more
openaire   +2 more sources

High-level expression of Aspergillus niger β-galactosidase in Ashbya gossypii [PDF]

, 2014
Ashbya gossypii has been recently considered as a host for the expression of recombinant proteins. The production levels achieved thus far were similar to those obtained with Saccharomyces cerevisiae for the same proteins.
Aguiar, Ashby, Dietrich, Domingues, Domingues, Domingues, Dünkler, Gietz, Gonçalves, Gueldener, Jiménez, Jiménez, Kato, Kaufmann, Knechtle, Kumar, Manzanares, Monschau, Nevalainen, Nevalainen, O'Connell, Oliveira, Oliveira, Park, Pompejus, Ramakrishnan, Revuelta Doval, Ribeiro, Ribeiro, Ribeiro, Ruohonen, Stahmann, Steiner, Steiner, Tajima, Wendland, Wickerham, Wright   +37 more
core   +1 more source

α-Galactosidase A fromPseudomonas fluorescenssubsp.cellulosa: cloning, high level expression and its role in galactomannan hydrolysis [PDF]

FEMS Microbiology Letters, 2000
A library of Pseudomonas fluorescens subsp. cellulosa genomic DNA, constructed in lambda ZAPII, was screened for alpha-D-galactosidase activity. The DNA inserts from six galactosidase-positive clones were rescued into plasmids. Restriction digestion and Southern analysis revealed that each of the plasmids contained a common DNA sequence.
J R, Halstead, M P, Fransen, R Y, Eberhart, A J, Park, H J, Gilbert, G P, Hazlewood   +5 more
openaire   +2 more sources

Development of stable flocculent Saccharomyces cerevisiae strain for continuous Aspergillus niger β-galactosidase production [PDF]

, 2007
A flocculent Saccharomyces cerevisiae strain was engineered to stably secrete Aspergillus niger β-galactosidase in a continuous high-cell-density bioreactor. The δ-sequences from the yeast retrotransposon Ty1 were used as target sites for the integration
Domingues, Lucília, Lima, Nelson, Oliveira, Carla Cristina Marques de, Silva, Nancy A. da, Teixeira, J. A.   +4 more
core   +1 more source

Site-directed mutation of β-galactosidase from Streptococcus thermophilus for galactooligosaccharide-enriched yogurt making

Journal of Dairy Science, 2022
: β-Galactosidase is one of the most important enzymes used in dairy processing. It converts lactose into glucose and galactose, and also catalyzes galactose to form galactooligosaccharides (GOS), so-called prebiotics.
J.C. Zhao, Y.L. Mu, X.Y. Gu, X.N. Xu, T.T. Guo, J. Kong   +5 more
doaj   +1 more source

Cloning and characterization of a novel α-galactosidase fromBifidobacterium breve203 capable of synthesizing Gal-α-1,4 linkage [PDF]

FEMS Microbiology Letters, 2008
A novel alpha-galactosidase gene (aga2) was cloned from Bifidobacterium breve 203. It contained an ORF of 2226-bp nucleotides encoding 741 amino acids with a calculated molecular mass of 81.5 kDa. The recombinant enzyme Aga2 was heterogeneously expressed, purified and characterized. Regarding substrate specificity for hydrolysis, Aga2 was highly active
Han, Zhao, Lili, Lu, Min, Xiao, Qinpeng, Wang, Yu, Lu, Chunhui, Liu, Peng, Wang, Hidehiko, Kumagai, Kenji, Yamamoto   +8 more
openaire   +2 more sources