Results 41 to 50 of about 101,136 (248)

Insertion of a 27 amino acid viral peptide in different zones ofEscherichia coliβ-galactosidase: Effects on the enzyme activity [PDF]

open access: yesFEMS Microbiology Letters, 1994
Seven internal, putatively exposed regions of Escherichia coli beta-galactosidase have been explored regarding their tolerance to insertions of large foreign peptides. Small sequence modifications, including amino acid substitutions and small deletions, were introduced into the lacZ gene to generate unique BamHI restriction sites. By using these mutant
A, Benito, A, Villaverde
openaire   +2 more sources

Senescence Associated β-galactosidase Staining

open access: yesBio-Protocol, 2012
Detection of senescent cells using a cytochemical assay was first described in 1995 (Dimri et al., 1995). The identification of senescent cells is based on an increased level of lysosomal β-galactosidase activity (Kurz et al., 2000).
Michael Eccles, Caiyun Li
doaj   +1 more source

Optimised N-acetyl-d-lactosamine synthesis using Thermus thermophilus β-galactosidase in bio-solvents

open access: yesTetrahedron, 2013
Synthesis of N-acetyl-D-lactosamine (Gal-β[1→4]GlcNAc, LacNAc) catalyzed by β-galactosidase from Thermus thermophilus (TTP0042) is affected by side reactions that give as result very low yields (about 20%) of LAcNAc when the reaction is performed in buffer.
Sandoval, Manuel   +4 more
openaire   +2 more sources

Study of muscle cell dedifferentiation after skeletal muscle injury of mice with a Cre-Lox system. [PDF]

open access: yesPLoS ONE, 2011
Dedifferentiation of muscle cells in the tissue of mammals has yet to be observed. One of the challenges facing the study of skeletal muscle cell dedifferentiation is the availability of a reliable model that can confidentially distinguish differentiated
Xiaodong Mu   +4 more
doaj   +1 more source

Biochemical characterization of digestive carbohydrases in Moroccan locust, Dociostaurus maroccanus Thunberg (Orthoptera: Acrididae) [PDF]

open access: yesArthropods, 2020
The Moroccan locust, Dociostaurus maroccanus (Thunberg), is an agricultural pest which has caused considerable agricultural damage and economic importance.
Bahareh Rafiei   +2 more
doaj  

Biochemical Insights into a Novel Family 2 Glycoside Hydrolase with Both β-1,3-Galactosidase and β-1,4-Galactosidase Activity from the Arctic

open access: yesMarine Drugs, 2023
A novel GH2 (glycoside hydrolase family 2) β-galactosidase from Marinomonas sp. BSi20584 was successfully expressed in E. coli with a stable soluble form.
Dianyi Li   +7 more
doaj   +1 more source

EXTRACTION AND CHARACTERIZATION OF Î’-GALACTOSIDASE ENZYME FROM ZIZIPHUS SPINA-CHRISTI FOR FOOD APPLICATIONS

open access: yesInternational Journal of Advanced Research
Galactosidaseis an industrially important enzymewitha number of applications in dairy, fermentation, and food industries. The study was carried out to extract and characterize the galactosidase enzymefromZiziphusspina-christi,a therapeutic herb. The enzyme was studied for pH, temperature sensitivity, and heavy metal effects on enzyme activity.
Omar Almuharib, Anmar Nazar Hasan
openaire   +1 more source

Overview of molecular signatures of senescence and associated resources: pros and cons

open access: yesFEBS Open Bio, EarlyView.
Cells can enter a stress response state termed cellular senescence that is involved in various diseases and aging. Detecting these cells is challenging due to the lack of universal biomarkers. This review presents the current state of senescence identification, from biomarkers to molecular signatures, compares tools and approaches, and highlights ...
Orestis A. Ntintas   +6 more
wiley   +1 more source

TNF receptor–related factor 3 inactivation promotes the development of intrahepatic cholangiocarcinoma through NF‐κB‐inducing kinase–mediated hepatocyte transdifferentiation

open access: yesHepatology, EarlyView., 2022
Abstract Background and Aims Intrahepatic cholangiocarcinoma (ICC) is a deadly but poorly understood disease, and its treatment options are very limited. The aim of this study was to identify the molecular drivers of ICC and search for therapeutic targets.
Yuto Shiode   +16 more
wiley   +1 more source

Study of β-Galactosidase Enzyme Activity Produced by Lactobacilli in Milk and Cheese [PDF]

open access: yesMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum, 2008
Background and objectiveLactose intolerance is a discomfort state that occurs in some people after ingestion of milk and it is due to insufficient amount of beta galactosidase in the human gut to digest lactose.
J. Nowroozi   +2 more
doaj  

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