Results 41 to 50 of about 132,836 (234)
Brain-specific gene expression by immortalized microglial cell-mediated gene transfer in the mammalian brain [PDF]
, 1998 The intra-arterial injection of immortalized microglia transfected with the lacZ gene, resulted in the expression of β-galactosidase in the rat brain at 48 h and the activity of β-galactosidase was detected for up to 3 weeks post-injection.
Hayakawa, Motoharu +5 more
core +1 more source
International Journal of Advanced ResearchGalactosidaseis an industrially important enzymewitha number of applications in dairy, fermentation, and food industries. The study was carried out to extract and characterize the galactosidase enzymefromZiziphusspina-christi,a therapeutic herb. The enzyme was studied for pH, temperature sensitivity, and heavy metal effects on enzyme activity.
Omar Almuharib, Anmar Nazar Hasan
openaire +1 more source
Effect of mechanical denaturation on surface free energy of protein powders [PDF]
, 2016 yesGlobular proteins are important both as therapeutic agents and excipients. However, their fragile native conformations can be denatured during pharmaceutical processing, which leads to modification of the surface energy of their powders and hence ...
Blagbrough, I.S. +4 more
core +2 more sources
Metabolic and functional consequences of cytosolic 5′-nucleotidase-IA overexpression in neonatal rat cardiomyocytes [PDF]
, 2003 Adenosine exerts a spectrum of energy-preserving actions on the heart negative chronotropic effects. The pathways leading to adenosine formation have remained controversial.
Curto, Maria de Los Angeles +3 more
core +1 more source
FEBS Letters, EarlyView.Dimeric pyruvate formate‐lyase cleaves pyruvate using a radical‐based mechanism. G734 serves as a radical storage location, and the radical is transferred to the catalytic C419 residue. Mutation of the C418‐C419 pair causes loss of enzyme activity, but does not impede radical introduction onto G734. Therefore, cis‐ but not trans‐radical transfer occurs
Michelle Kammel +2 more
wiley +1 more source
Construction and characterization of a recombinant tripartite enzyme, galactose dehydrogenase/β-galactosidase/galactokinase [PDF]
, 1990 The in-frame gene fusion between 3 enzymes, galactose dehydrogenase, β-galactosidase and galactokinase, is described. The purified artificial tripartite enzyme displayed all three enzymic activities.
Bülow, Leif +2 more
core +1 more source
CreA-mediated carbon catabolite repression of $beta;-galactosidase formation in Aspergillus nidulans is growth rate dependent [PDF]
FEMS Microbiology Letters, 2004 Carbon catabolite repression by the CreA-transcriptional repressor is widespread in filamentous fungi, but the mechanism by which glucose triggers carbon catabolite repression is still poorly understood. We investigated the hypothesis that the growth rate on glucose may control CreA-dependent carbon catabolite repression by using glucose-limited ...
Hedvig Ilyés +6 more
openaire +3 more sources
Molecular Oncology, EarlyView.Chronic TGF‐β exposure drives epithelial HCC cells from a senescent state to a TGF‐β resistant mesenchymal phenotype. This transition is characterized by the loss of Smad3‐mediated signaling, escape from senescence, enhanced invasiveness and metastatic potential, and upregulation of key resistance modulators such as MARK1 and GRM8, ultimately promoting
Minenur Kalyoncu +11 more
wiley +1 more source
Understanding the Effects of Lactose Hydrolysis Modeling on the Main Oligosaccharides in Goat Milk Whey Permeate. [PDF]
, 2019 Enzymatic hydrolysis of lactose is a crucial step to improve the efficiency and selectivity of membrane-based separations toward the recovery of milk oligosaccharides free from simple sugars.
Barile, Daniela +5 more
core +2 more sources
, 2016 Additional file 1. Stoichiometric and carbon atom transition model for P. pastoris.
Liu, Ping +7 more
openaire +1 more source