Results 31 to 40 of about 6,427 (151)

Liquid chromatography-mass spectrometry utilizing multi-stage fragmentation for the identification of oxysterolss⃞

Journal of Lipid Research, 2007
In humans, the brain accounts for about 20% of the body's free cholesterol, most of which is synthesized de novo in brain. To maintain cholesterol balance throughout life, cholesterol becomes metabolized to 24S-hydroxycholesterol, principally in neurons.
Kersti Karu, Martin Hornshaw, Gary Woffendin, Karl Bodin, Mats Hamberg, Gunvor Alvelius, Jan Sjövall, John Turton, Yuqin Wang, William J. Griffiths   +9 more
doaj   +1 more source

Analysis of bioactive oxysterols in newborn mouse brain by LC/MS

Journal of Lipid Research, 2012
Unesterified cholesterol is a major component of plasma membranes. In the brain of the adult, it is mostly found in myelin sheaths, where it plays a major architectural role.
Anna Meljon, Spyridon Theofilopoulos, Cedric H.L. Shackleton, Gordon L. Watson, Norman B. Javitt, Hans-Joachim Knölker, Ratni Saini, Ernest Arenas, Yuqin Wang, William J. Griffiths   +9 more
doaj   +1 more source

Oxidation of 7-dehydrocholesterol and desmosterol by human cytochrome P450 46A1[S]

Journal of Lipid Research, 2014
Cytochrome P450 (P450 or CYP) 46A1 is expressed in brain and has been characterized by its ability to oxidize cholesterol to 24S-hydroxycholesterol. In addition, the same enzyme is known to further oxidize 24S-hydroxycholesterol to the 24,25- and 24,27 ...
Sandeep Goyal, Yi Xiao, Ned A. Porter, Libin Xu, F. Peter Guengerich   +4 more
doaj   +1 more source

High levels of (24S)-24-hydroxycholesterol 3-sulfate, 24-glucuronide in the serum and urine of children with severe cholestatic liver disease

Journal of Lipid Research, 1997
Extracts of urine and serum from children with cholestatic liver disease were analyzed by fast atom bombardment (FAB) mass spectrometry. About half of all spectra showed a peak at m/z 657, compatible with the presence of a glucuronidated cholestenediol ...
L J Meng, W J Griffiths, H Nazer, Y Yang, J Sjövall   +4 more
doaj   +1 more source

Key regulatory oxysterols in liver: analysis as Δ4-3-ketone derivatives by HPLC and response to physiological perturbations

Journal of Lipid Research, 2001
A number of oxysterols have been implicated in metabolic regulation. Key among these are (24S),25-epoxycholesterol and (24S)-hydroxycholesterol, high affinity ligands for the nuclear transcription factor liver X receptor α; 27-hydroxycholesterol, a bile ...
Zhe Zhang, Dansu Li, Daniel E. Blanchard, Steven R. Lear, Sandra K. Erickson, Thomas A. Spencer   +5 more
doaj   +1 more source

On the rate of translocation in vitro and kinetics in vivo of the major oxysterols in human circulation

Journal of Lipid Research, 2002
Oxysterols possess powerful biological activities. Some of their effects on the regulation of key enzymes are similar to those of cholesterol, but are much more potent.
Steve Meaney, Karl Bodin, Ulf Diczfalusy, Ingemar Björkhem   +3 more
doaj   +1 more source

The Effect of 24S-Hydroxycholesterol on Cholesterol Homeostasis in Neurons: Quantitative Changes to the Cortical Neuron Proteome

, 2016
In humans, the brain represents only about 2% of the body’s mass but contains about one-quarter of the body’s free cholesterol. Cholesterol is synthesized de novo in brain and removed by metabolism to oxysterols.
Sabina Muneton (2445274), Jasmina N. Jovanovic (2445277), William J. Griffiths (2382700), Yuqin Wang (2382697), Jan Sjövall (2445271)   +4 more
core   +3 more sources

Highly sensitive quantification of key regulatory oxysterols in biological samples by LC-ESI-MS/MSs⃞

Journal of Lipid Research, 2009
We describe a highly sensitive and specific method for the quantification of key regulatory oxysterols in biological samples. This method is based upon a stable isotope dilution technique by liquid chromatography-tandem mass spectrometry (LC-MS/MS ...
Akira Honda, Kouwa Yamashita, Takashi Hara, Tadashi Ikegami, Teruo Miyazaki, Mutsumi Shirai, Guorong Xu, Mitsuteru Numazawa, Yasushi Matsuzaki   +8 more
doaj   +1 more source

Plasma levels of 24S-hydroxycholesterol in patients with neurological diseases

, 2000
The brain is the exclusive or almost exclusive site of formation of 24S-hydroxycholesterol and we have shown that the circulating level of 24S-hydroxycholesterol is dependent upon the relation between cerebral production and hepatic clearance.
Siden, A, Bjorkhem, I, Lutjohann, D, Bretillon, L, Wahlund, L O, Minthon, Lennart, Diczfalusy, U, Crisby, M, Hillert, J, Groth, C G   +9 more
core   +1 more source

24S-hydroxycholesterol in cerebrospinal fluid is elevated in early stages of dementia

, 2002
The brain is the most cholesterol-rich organ in the human body. Accumulation of excess cholesterol in hippocampal neurons promotes the cleavage of the amyloid precursor protein (APP) into amyloidogenic components with the consequence of the acceleration ...
Jessen, F, Bagli, M., Lütjohann, D., Jessen, F., von Bergmann, K., Papassotiropoulos, A., Buschfort, R, Locatelli, S, Von Bergmann, K, Buschfort, R., Papassotiropoulos, A, Ptok, U, Locatelli, S., Heun, R., Heun, R, Lutjohann, D, Bjorkhem, A, von, Bergmann K, Bagli, M, Heun, Reinhard, Björkhem, I., Ptok, U., Björkhem, I,   +22 more
core   +1 more source