Results 61 to 70 of about 23,918 (199)

Cryo‐EM Structure Guided Engineering of Botulinum Neurotoxin A With Advanced Receptor Binding Affinity and Therapeutical Benefits

Advanced Science, EarlyView.
The butterfly unfolded wing in an open form structure of botulinum neurotoxin type A (BoNT/A) at physiological‐state was confirmed at 2.85 Å resolution by cryo‐electron microscopy (cryo‐EM). Structure‐guided protein engineering significantly enhanced the receptor‐binding affinity, therapeutic efficacy, and safety of the engineered toxin variants ...
Wenrui Wang, Zhaxi Zerang, Linjin You, Ziye Liu, Rong Nie, Fuwei Qi, Fenfen Gao, Chengmu Zhao, Wantong Ma, Jinghan He, Xiaoru Wang, Shanquan Wu, Bo Liu, Xinyao Liu, Dongsheng Lei, Dejuan Zhi, Dongsheng Wang   +16 more
wiley   +1 more source

Distinct Spatiotemporal Distribution of Bacterial Toxin-Produced Cellular cAMP Differentially Inhibits Opsonophagocytic Signaling

Toxins, 2019
Myeloid phagocytes have evolved to rapidly recognize invading pathogens and clear them through opsonophagocytic killing. The adenylate cyclase toxin (CyaA) of Bordetella pertussis and the edema toxin (ET) of Bacillus anthracis are both calmodulin ...
Shakir Hasan, Waheed Ur Rahman, Peter Sebo, Radim Osicka   +3 more
doaj   +1 more source

Bordetella adenylate cyclase toxin differentially modulates toll-like receptor-stimulated activation, migration and T cell stimulatory capacity of dendritic cells. [PDF]

PLoS ONE, 2014
Adenylate cyclase toxin (CyaA) is a key virulence factor of the whooping cough agent Bordetella pertussis. The toxin targets CD11b-expressing phagocytes and delivers into their cytosol an adenylyl cyclase (AC) enzyme that subverts cellular signaling by ...
Irena Adkins, Jana Kamanova, Aneta Kocourkova, Martina Svedova, Jakub Tomala, Hana Janova, Jiri Masin, Barbora Chladkova, Ladislav Bumba, Marek Kovar, Padraig J Ross, Ludmila Tuckova, Radek Spisek, Kingston H G Mills, Peter Sebo   +14 more
doaj   +1 more source

Effect of phenylephrine and prazosin on the somatostatinergic system in the rat frontoparietal cortex [PDF]

, 1994
Somatostatin (SS) and noradrenaline (NA) are distributed in the rat cerebral cortex, and seizure activity is one of the aspects of behavior affected by both neurotransmitters.
Arilla Ferreiro, Eduardo, López Sañudo, Susana, Martín Espinosa, Ángela, Rodríguez Martín, Eulalia   +3 more
core   +3 more sources

Identification of MEF2A, MEF2C, and MEF2D interactomes in basal and Fsk‐stimulated mouse MA‐10 Leydig cells

Andrology, EarlyView.
Abstract Background Myocyte enhancer factor 2 transcription factors regulate essential transcriptional programs in various cell types. The activity of myocyte enhancer factor 2 factors is modulated through interactions with cofactors, chromatin remodelers, and other regulatory proteins, which are dependent on cell context and physiological state.
Karine de Mattos, Marie‐Pier Scott‐Boyer, Arnaud Droit, Robert S. Viger, Jacques J. Tremblay   +4 more
wiley   +1 more source

Essential role of CFTR in PKA-dependent phosphorylation, alkalinization, and hyperpolarization during human dperm capacitation [PDF]

, 2017
Mammalian sperm require to spend a limited period of time in the female reproductive tract to become competent to fertilize in a process called capacitation.
Austin, Berger, Bobadilla, Buffone, Buffone, Buffone, Buffone, Chang, Chen, Chen, Chen, Chávez, Chávez, De Jonge, De La Vega-Beltran, Demarco, Diao, Dirami, Dostmann, Ellsworth, Escoffier, Esteves, Figueiras-Fierro, Gabriel, Gadsby, Hernández-González, Ishiguro, José, Kase, Kujala, Köhn, l'Hoste, Laemmli, Leclerc, Li, Lishko, Lochner, Luconi, López-González, Ma, Melis, Mortimer, Muanprasat, Navarrete, Neill, Nishigaki, Nolan, Okamura, Rode, Schulz, Sheppard, Sorum, Stival, Stival, Tabcharani, Tang, Towbin, Visconti, Visconti, Wennemuth, Wertheimer, Xu, Zeng, Zeng   +63 more
core   +1 more source

Mechanism of activation of adenylate cyclase by cholera toxin. [PDF]

Proceedings of the National Academy of Sciences, 1975
Cholera toxin (choleragen) can stimulate adenylate cyclase [EC 4.6.1.1; ATP pyrophosphate-lyase (cyclizing)] activity in whole particulate fractions or purified plasma membranes of homogenates of isolated fat cells provided special precautions are taken to stabilize the enzyme during the required preincubation period. As observed with intact cells, the
N, Sahyoun, P, Cuatrecasas
openaire   +2 more sources

Novel approaches for drug development against chronic primary pain: A systematic review

British Journal of Pharmacology, EarlyView.
Abstract Chronic primary pain (CPP) persisting for more than 3 months, associated with significant emotional distress without any known underlying cause, is an unmet medical need. Traditional or adjuvant analgesics do not provide satisfactory pain relief for a great proportion of these patients.
Valéria Tékus, Éva Borbély, Andreas Goebel, Ralf Baron, Zsófia Hajna, Zsuzsanna Helyes   +5 more
wiley   +1 more source

Differential segment‐specific signalling pathways for guanylate cyclase C‐activated anion secretion in murine ileocolon

British Journal of Pharmacology, EarlyView.
Abstract Background and Purpose Guanylate cyclase‐C (GC‐C) is the receptor for endogenous (uro)guanylin peptides, bacterial toxins and pharmacological analogues. Receptor activation leads to intestinal fluid loss, but also activates an antiproliferative pathway and is a promising target in colorectal cancer therapy.
Renjie Xiu, Johannes Reiner, Franz Hofmann, Azam Salari, Ursula Seidler   +4 more
wiley   +1 more source

Distinct role of clathrin-mediated endocytosis in the functional uptake of cholera toxin [PDF]

, 2007
The involvement of the clathrin-mediated endocytic internalization route in the uptake of cholera toxin (CT) was investigated using different cell lines, including the human intestinal Caco-2 and T84 cell lines, green monkey Vero cells, SH-SY5Y ...
De Wolf, Marc JS, Lagrou, Albert R, Vanden Broeck, Davy   +2 more
core   +1 more source