The pathogenicity of or aerobactin-positive in infected mice
Objectives To investigate the pathogenicity of Klebsiella pneumoniae (KPN) possessing rmpA or the aerobactin gene in infected mice. Methods BALB/c mice were divided into four groups (n = 10 per group) and infected with: string test-positive and rmpA ...
Guili Li +3 more
doaj +2 more sources
Tracking key virulence loci encoding aerobactin and salmochelin siderophore synthesis in Klebsiella pneumoniae [PDF]
Background Klebsiella pneumoniae is a recognised agent of multidrug-resistant (MDR) healthcare-associated infections; however, individual strains vary in their virulence potential due to the presence of mobile accessory genes.
Margaret M. C. Lam +6 more
doaj +10 more sources
Antimicrobial Resistance, Virulence, and Genomic Characteristics of <i>Klebsiella pneumoniae</i> Isolated From Four Host Sources in Northeast China. [PDF]
Klebsiella pneumoniae is an important emerging pathogen leading to serious clinical outcomes as the multidrug‐resistant (MDR) isolates arise. While K. pneumoniae is a well‐known pathogen in human clinical settings, its risks in nonhuman hosts and potential for cross‐species transmission remain poorly understood. In this study, we performed whole‐genome
Xu Q +8 more
europepmc +2 more sources
Aerobactin genes in Shigella spp [PDF]
Aerobactin, a hydroxamate iron transport compound, is synthesized by some, but not all, Shigella species. Conjugation and hybridization studies indicated that the genes for the synthesis and transport of aerobactin are linked and are found on the chromosome of Shigella flexneri, S. boydii, and S. sonnei. The genes were not found in S. dysenteriae.
K M, Lawlor, S M, Payne
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Novel Aerobactin Receptor in Klebsiella pneumoniae [PDF]
Several Klebsiella pneumoniae strains which produced enterochelin but not aerobactin were nevertheless sensitive to cloacin DF13. In contrast, a strain of serotype K1:O1 which produced both siderophores was cloacin-resistant. Loss by mutation of the O1 but not K1 antigen rendered this strain cloacin-sensitive, indicating that the O1 antigen prevented ...
P, Williams +4 more
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Biochemical genetics of aerobactin biosynthesis in Escherichia coli [PDF]
Single-gene mutants of Escherichia coli defective in aerobactin biosynthesis were incubated under non-growing conditions for 2 h with radiolabelled lysine. Analysis of the intermediates produced suggested that acetylation of lysine may be the first step in aerobactin production.
Steven Ford +2 more
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Aerobactin genes in clinical isolates of Escherichia coli [PDF]
The location of the aerobactin gene complex on either the chromosome or plasmid was determined in eight aerobactin-positive clinical isolates of Escherichia coli by Southern hybridization analysis, using as probes the cloned aerobactin genes from the ColV-K30 plasmid.
A, Bindereif, J B, Neilands
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Production of the siderophore aerobactin by a halophilic pseudomonad [PDF]
A bacterial strain, isolated from a cyanobacterial culture, was identified as Pseudomonas sp. strain X40. Under iron-limiting conditions, the Pseudomonas sp. produced aerobactin, a dihydroxamate siderophore previously found only in the family Enterobacteriaceae. Aerobactin was identified by electrophoretic mobility, spectrophotometric titration, proton
J S, Buyer, V, de Lorenzo, J B, Neilands
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Enterobacter bugandensis: a novel enterobacterial species associated with severe clinical infection [PDF]
Nosocomial pathogens can cause life-threatening infections in neonates and immunocompromised patients. E. bugandensis (EB-247) is a recently described species of Enterobacter, associated with neonatal sepsis.
Bunk, Boyke +20 more
core +2 more sources
Subcloning of the cloacin DF13/aerobactin receptor protein and identification of a pColV-K30-determined polypeptide involved in ferric-aerobactin uptake [PDF]
A plasmid containing a pColV-K30 fragment that encoded only for the cloacin DF13/aerobactin receptor protein was constructed. Escherichia coli cells harboring this plasmid were sensitive to cloacin DF13 but were unable to take up ferric-aerobactin. Another pColV-K30-determined polypeptide (molecular weight, 50,000), localized in the membrane fraction ...
W J, Krone +4 more
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