Production of aerobactin by some species of the genus Yersinia [PDF]
Seventy-seven isolates of Yersinia species were examined for evidence of hydroxamate biosynthesis. A total of 7 of 12 Y. frederiksenii, 1 of 5 Y. kristensenii, and 5 of 5 Y. intermedia isolates synthesized an hydroxamate species which was chromatographically and electrophoretically identical to aerobactin. Proof that this material was indeed aerobactin
S J, Stuart +2 more
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The aerobactin-mediated iron uptake system in Escherichia coli K-12.
Some strains of E. coli secrete a low molecular weight compound, aerobactin, with a high affinity for ferric iron. After complexing ferric iron aerobactin may be transported back into the cell and used as a source of iron.
Karl Graham. Wooldridge (7644806)
core +6 more sources
Aerobactin genes in clinical isolates of Escherichia coli [PDF]
The location of the aerobactin gene complex on either the chromosome or plasmid was determined in eight aerobactin-positive clinical isolates of Escherichia coli by Southern hybridization analysis, using as probes the cloned aerobactin genes from the ColV-K30 plasmid.
A, Bindereif, J B, Neilands
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Multidrug-resistant hypervirulent Klebsiella pneumoniae (MDR-hvKP) has been increasingly reported and is now recognized as a significant threat to public health; however, characterization of MDR-hvKP has not been systematically investigated.
Zhi-wei Lin +12 more
doaj +1 more source
Biochemical genetics of aerobactin biosynthesis in Escherichia coli [PDF]
Single-gene mutants of Escherichia coli defective in aerobactin biosynthesis were incubated under non-growing conditions for 2 h with radiolabelled lysine. Analysis of the intermediates produced suggested that acetylation of lysine may be the first step in aerobactin production.
Steven Ford +2 more
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Subcloning of the cloacin DF13/aerobactin receptor protein and identification of a pColV-K30-determined polypeptide involved in ferric-aerobactin uptake [PDF]
A plasmid containing a pColV-K30 fragment that encoded only for the cloacin DF13/aerobactin receptor protein was constructed. Escherichia coli cells harboring this plasmid were sensitive to cloacin DF13 but were unable to take up ferric-aerobactin. Another pColV-K30-determined polypeptide (molecular weight, 50,000), localized in the membrane fraction ...
W J, Krone +4 more
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Activity and specificity of a mouse monoclonal antibody to ferric aerobactin [PDF]
We isolated a monoclonal antibody directed against the ferric complex of aerobactin purified from Escherichia coli KH576. This antibody, which we designated MAb AERO1, was identified as an immunoglobulin G, subtype 2. A competitive enzyme-linked immunosorbent assay with MAb AERO1 had a limit of 10 nM for the detection of purified ferric aerobactin and ...
D, le Roy +6 more
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ShiF acts as an auxiliary factor of aerobactin secretion in meningitis Escherichia coli strain S88
Background The neonatal meningitis E. coli (NMEC) strain S88 carries a ColV plasmid named pS88 which is involved in meningeal virulence. Transcriptional analysis of pS88 in human serum revealed a strong upregulation of an ORF of unknown function: shiF ...
Mathieu Genuini +6 more
doaj +1 more source
Genomic insights into virulence factors affecting tissue-invasive Klebsiella pneumoniae infection
Background The key virulence factors responsible for hypervirulent Klebsiella pneumoniae (hvKp) infection remains elusive. Methods We analyzed K. pneumoniae isolates collected between 2017 and 2019 and defined hvKp as a pyogenic infection.
Takashi Matono +4 more
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Aerobactin synthesis genes iucA and iucC contribute to the pathogenicity of avian pathogenic Escherichia coli O2 strain E058. [PDF]
Aerobactin genes are known to be present in virulent strains and absent from avirulent strains, but contributions of iucC and iucA, which are involved in aerobactin synthesis, to the pathogenicity of avian pathogenic Escherichia coli (APEC) have not been
Jielu Ling +7 more
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