Results 41 to 50 of about 27,782 (234)

The in vitro effects of dexamethasone, insulin and triiodothyronine on degenerative human intervertebral disc cells under normoxic and hypoxic conditions [PDF]

European Cells & Materials, 2011
Degeneration of intervertebral discs (IVD) is one of the main causes of back pain and tissue engineering has been proposed as a treatment. Tissue engineering requires the use of highly expensive growth factors, which might, in addition, lack regulatory ...
A Bertolo, L Ettinger, N Aebli, D Haschtmann, M Baur, U Berlemann, SJ Ferguson, JV Stoyanov   +7 more
doaj  

Analysis of Aggrecan Glycanation by Western Blot in Cell Culture

, 2023
: Several experimental protocols are available to study the synthesis and secretion of proteoglycans in health and diseases, but there are few methods to analyse the intracellular processing of these macromolecules.
Rossi, Antonio, Paganini, Chiara, Leone, Alessandra, Gramegna Tota, Chiara   +3 more
core   +1 more source

Patients with rheumatoid arthritis have an altered circulatory aggrecan profile [PDF]

, 2008
Background Rheumatoid arthritis (RA) is a chronic auto-immune disease with extensive articular cartilage destruction. Aggrecan depletion, mediated by aggrecanases is one of the first signs of early cartilage erosion.
Hein Gert, Sondergaard, Bodil, C, Sumer, Eren, U, Christian Pedersen, Per Qvist, Mueller, Andreas, Pedersen Christian, Madsen, Suzi, H, Pedersen, Christian, Karsdal Morten A, Morten A Karsdal, Qvist Per, Gert Hein, Suzi H Madsen, Bodil C Sondergaard, Rousseau Jean C, Rousseau, Jean,, Neumann, Thomas, Thomas Neumann, Sumer Eren U, Hein, Gert, Karsdal, Morten, A, Andreas Mueller, Karsdal, Morten,, Rousseau, Jean, C, Neumann Thomas, Madsen Suzi H, Sondergaard Bodil C, Sondergaard, Bodil,, Mueller Andreas, Sumer, Eren,, Delmas, Pierre, Eren U Sumer, Madsen, Suzi,, Delmas Pierre, Qvist, Per, Pierre Delmas, Jean C Rousseau   +37 more
core   +2 more sources

Visfatin promotes intervertebral disc degeneration by inducing IL-6 expression through the ERK/JNK/p38 signalling pathways

Adipocyte, 2021
Visfatin reportedly induces the expression of proinflammatory cytokines. Severe grades of intervertebral disc disease (IVDD) exhibit higher expression of visfatin than mild ones.
Haitao Cui, Xianfa Du, Caijun Liu, Shunlun Chen, Haowen Cui, Hui Liu, Jianru Wang, Zhaomin Zheng   +7 more
doaj   +1 more source

Catabolism of aggrecan, decorin and biglycan in tendon [PDF]

Biochemical Journal, 2000
We have examined the catabolism of the proteoglycans aggrecan, decorin and biglycan in fresh tendon samples and in explant cultures of tissue from the tensional and compressed regions of young and mature bovine tendons. A panel of well-characterized antibodies that recognize glycosaminoglycan or protein (linear or neoepitope) sequences was used to ...
S G, Rees, C R, Flannery, C B, Little, C E, Hughes, B, Caterson, C M, Dent   +5 more
openaire   +2 more sources

Dynamic Loading Regulates Meniscus‐Like Matrix Production in Human Mesenchymal Stromal Cell‐Seeded PET Scaffolds

Advanced Healthcare Materials, EarlyView.
Dynamic compression enhances mesenchymal stromal cell proliferation in nonwoven PET scaffolds under chondrogenic differentiation conditions and triggers mechanosensitive transcriptional programs associated with extracellular matrix remodeling. These findings highlight the potential of mechanically stimulated PET scaffolds as a promising platform for ...
Graciosa Quelhas Teixeira, Luisa de Roy, Anna‐Lotta Feldmeier, Mubashir Ahmad, Sofia Pilão, Maria Ahrens, Anita Ignatius, Carsten Linti, Andreas Martin Seitz   +8 more
wiley   +1 more source

Immunohistochemical staining for aggrecan.

, 2019
(A) Immunostaining for aggrecan in the IVD from control non-smoking (N8) and smoking (S8) rats for 8 weeks. Upper and lower panels represent low and high magnification, respectively. Bars indicate 500 μm and 100 μm, respectively.
Yasuaki Tokuhashi (7314074), Masahiro Nakahashi (7314071), Mariko Esumi (2551771)   +2 more
core   +1 more source

Aggrecan degradation in health and disease [PDF]

The main aggrecan catabolite, found in samples of synovial fluid from patients with arthritis, and released from cartilage explant cultures exposed to IL-1, both have the amino-terminal amino acid sequence 374ARGSV...
Rees, Alison Jane
core   +6 more sources

Biomimetic Proteoglycans for Intervertebral Disc (IVD) Regeneration

Biomimetics
Intervertebral disc degeneration, which leads to low back pain, is the most prevalent musculoskeletal condition worldwide, significantly impairing quality of life and imposing substantial socioeconomic burdens on affected individuals.
Neha Chopra, James Melrose, Zi Gu, Ashish D. Diwan   +3 more
doaj   +1 more source

Poly I:C Activated Microglia Disrupt Perineuronal Nets and Modulate Synaptic Balance in Primary Hippocampal Neurons in vitro

Frontiers in Synaptic Neuroscience, 2021
Perineuronal nets (PNNs) are specialized, reticular structures of the extracellular matrix (ECM) that can be found covering the soma and proximal dendrites of a neuronal subpopulation.
David Wegrzyn, Nadja Freund, Andreas Faissner, Georg Juckel   +3 more
doaj   +1 more source