FEBS Open Bio, EarlyView.CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point +7 more
wiley +1 more source
Review of: "[Review] The antibacterial activity of Allium sativum, Thymus vulgaris, Origanum vulgare, Curcuma longa, Rosmarinus officinalis, and Cinnamomum species against various antibiotic-resistant strains of bacteria: A Literature Review" [PDF]
, 2023 S. L.P.
openalex +1 more source
Development and application of an antibiotic spectrum index for benchmarking antibiotic selection patterns across hospitals [PDF]
, 2017 Standard metrics for antimicrobial use consider volume but not spectrum of antimicrobial prescribing. We developed an antibiotic spectrum index (ASI) to classify commonly used antibiotics based on activity against important pathogens.
Gerber, Jeffrey S +5 more
core +2 more sources
Promiscuous stimulation of HSP70 ATPase activity by parasite‐derived J‐domains
FEBS Open Bio, EarlyView.The malaria parasite Plasmodium falciparum exports three highly homologous yet functionally divergent J‐domain proteins into human erythrocytes. Here, we show that J‐domains isolated from all three proteins effectively stimulate the ATPase activity of both endogenous host and exported parasite HSP70 chaperones.
Julian Barth +6 more
wiley +1 more source
Microbiology SpectrumThere is an urgent need for accurate and fast diagnostic tests to identify carbapenemase-producing bacteria. Here, we evaluated a colorimetric imipenem hydrolysis test, called the CARBA PAcE test, to detect carbapenemase-producing Gram-negative bacteria (
Nadya Rakovitsky +8 more
doaj +1 more source
Reducing Antibiotic Use in Pediatric Upper Respiratory Infection: A Multifaceted Parent-Clinician Approach [PDF]
, 2017 The goal of this QI initiative was to decrease inappropriate antibiotic for the treatment of pediatric upper respiratory infection (URI) in the retail clinic setting. The approach included the use of a protocol to treat viral upper respiratory illness, a
Lucas, Sydnee H, DNP, APRN +1 more
core +1 more source
Host metabolites stimulate the bacterial proton motive force to enhance the activity of aminoglycoside antibiotics [PDF]
, 2019 Antibiotic susceptibility of bacterial pathogens is typically evaluated using in vitro assays that do not consider the complex host microenvironment.
Coenye, Tom +10 more
core +6 more sources
FEBS Open Bio, EarlyView.We first identified functional murine mitochondrial N‐formyl peptides (MT‐FPs) and investigated their effects on the in vitro myeloid‐derived suppressor cell (MDSC) generation from bone marrow cells. We demonstrated that MT‐FPs acted directly on bone marrow cells to promote MDSC generation and modulated the polymorphonuclear (PMN)‐MDSC/monocyte (M ...
Miyako Ozawa +2 more
wiley +1 more source
Rapid identification of capsulated Acinetobacter baumannii using a density-dependent gradient test
BMC Microbiology, 2020 Background Gram-negative bacterial capsules are associated with production of carbohydrates, frequently resulting in a mucoid phenotype. Infections caused by capsulated or mucoid A. baumannii are associated with increased clinical severity. Therefore, it
Hadas Kon +4 more
doaj +1 more source
Promoting Antibiotic Stewardship [PDF]
, 2017 Antibiotics are not always prescribed optimally in the outpatient setting for common respiratory illnesses. Providers also spend time reiterating the same facts to patients about antibiotics and why their use is not warranted for their common cold ...
Patel, Saurabh
core +1 more source