Prediction of aptamer-target interacting pairs with pseudo-amino acid composition. [PDF]
PLoS ONE, 2014 Aptamers are oligonucleic acid or peptide molecules that bind to specific target molecules. As a novel and powerful class of ligands, aptamers are thought to have excellent potential for applications in the fields of biosensing, diagnostics and ...
Bi-Qing Li +5 more
doaj +1 more source
Electrochemical Aptasensor for Detection of Dopamine [PDF]
, 2020 This work presents a proof of concept of a novel, simple, and sensitive method of detection of dopamine, a neurotransmitter within the human brain. We propose a simple electrochemical method for the detection of dopamine using a dopamine-specific aptamer
Abu-Ali, Hisham +4 more
core +1 more source
Three-dimensional modeling of single stranded DNA hairpins for aptamer-based biosensors. [PDF]
, 2017 Aptamers consist of short oligonucleotides that bind specific targets. They provide advantages over antibodies, including robustness, low cost, and reusability.
Jeddi, Iman, Saiz, Leonor
core +2 more sources
Stepping towards highly flexible aptamers: enzymatic recognition studies of unlocked nucleic acid nucleotides [PDF]
Chemical Communications, 2012 Enzymatic recognition of unlocked nucleic acid (UNA) nucleotides was successfully accomplished. Therminator DNA polymerase was found to be an efficient enzyme in primer extension reactions. Polymerase chain reaction (PCR) amplification of a 81 mer UNA-modified DNA library was efficiently achieved by KOD DNA polymerase.
Dubois, C. +4 more
openaire +4 more sources
Construction of a Bivalent Thrombin Binding Aptamer and Its Antidote with Improved Properties
Molecules, 2017 Aptamers are short synthetic DNA or RNA oligonucleotides that adopt secondary and tertiary conformations based on Watson–Crick base-pairing interactions and can be used to target a range of different molecules.
Quintin W. Hughes +4 more
doaj +1 more source
Monitoring genomic sequences during SELEX using high-throughput sequencing: neutral SELEX. [PDF]
PLoS ONE, 2010 BACKGROUND: SELEX is a well established in vitro selection tool to analyze the structure of ligand-binding nucleic acid sequences called aptamers. Genomic SELEX transforms SELEX into a tool to discover novel, genomically encoded RNA or DNA sequences ...
Bob Zimmermann +4 more
doaj +1 more source
Enzymatic Synthesis of Vancomycin-Modified DNA
Molecules, 2022 Many potent antibiotics fail to treat bacterial infections due to emergence of drug-resistant strains. This surge of antimicrobial resistance (AMR) calls in for the development of alternative strategies and methods for the development of drugs with ...
Chiara Figazzolo +4 more
doaj +1 more source
Mechanistic insights into an engineered riboswitch: a switching element which confers riboswitch activity [PDF]
, 2010 While many different RNA aptamers have been identified that bind to a plethora of small molecules only very few are capable of acting as engineered riboswitches.
Duchardt-Ferner, Elke +6 more
core +1 more source
Mature miRNAs form secondary structure, which suggests their function beyond RISC. [PDF]
PLoS ONE, 2014 The generally accepted model of the miRNA-guided RNA down-regulation suggests that mature miRNA targets mRNA in a nucleotide sequence-specific manner.
Agnieszka Belter +6 more
doaj +1 more source
Molecular Therapy: Nucleic Acids, 2021 Post-SELEX modification of DNA aptamers is an established strategy to improve their affinity or inhibitory characteristics. In this study, we examined the possibility of increasing the recognition interface between the thrombin-binding aptamer HD1 (TBA ...
Igor Smirnov +4 more
doaj +1 more source