HIV-1 reverse transcriptase mutations that confer decreased in vitro susceptibility to anti-RT DNA aptamer RT1t49 confer cross resistance to other anti-RT aptamers but not to standard RT inhibitors [PDF]
, 2005 RNA and DNA aptamers specific for HIV-1 reverse transcriptase (RT) can inhibit reverse transcription in vitro. RNA aptamers have been shown to potently block HIV-1 replication in culture.
Fisher, Timothy S +2 more
core +3 more sources
Towards the selection of phosphorothioate aptamers [PDF]
European Journal of Biochemistry, 2000 The high affinity of a given nucleic acid for a protein ligand can be used to isolate specific inhibitors of enzymes involved in pathological situations. The latter property is the basis of the SELEX (systematic evolution of ligands by exponential enrichment) technique.
M L, Andreola +4 more
openaire +2 more sources
Kinetic analysis of an efficient DNA-dependent TNA polymerase. [PDF]
, 2005 alpha-l-Threofuranosyl nucleoside triphosphates (tNTPs) are tetrafuranose nucleoside derivatives and potential progenitors of present-day beta-d-2'-deoxyribofuranosyl nucleoside triphosphates (dNTPs).
Chaput, John C +6 more
core +2 more sources
Identification of Allosteric Nucleotide Sites of Tetramethylrhodamine-Labeled Aptamer for Noncompetitive Aptamer-Based Fluorescence Anisotropy Detection of a Small Molecule, Ochratoxin A [PDF]
Analytical Chemistry, 2013 Aptamer-based fluorescence anisotropy (FA) assay combines the advantages of affinity aptamers in good stability, easy generation, and facile labeling and the benefits of FA in homogeneous analysis, such as robustness, simplicity, and high reproducibility.
Qiang, Zhao, Qin, Lv, Hailin, Wang
openaire +2 more sources
Aptamer based diagnosis of crimean-congo hemorrhagic fever from clinical specimens
Scientific Reports, 2021 Crimean-Congo hemorrhagic fever (CCHF) is an acute viral zoonotic disease. The widespread geographic distribution of the disease and the increase in the incidence of the disease from new regions, placed CCHF in a list of public health emergency contexts.
Tahmineh Jalali +3 more
doaj +1 more source
Upgrading SELEX Technology by Using Lambda Exonuclease Digestion for Single-Stranded DNA Generation
Molecules, 2009 The generation of single-stranded DNA (ssDNA) molecules plays a key role in the SELEX (Systematic Evolution of Ligands by EXponential enrichment) combinatorial chemistry process and numerous molecular biology techniques and applications, such as DNA ...
Meltem Avci-Adali +4 more
doaj +1 more source
Pharmaceuticals, 2022 Von Willebrand Factor (VWF) plays a critical role in thrombus formation, stabilization, and propagation. Previous studies have demonstrated that targeted inhibition of VWF induces thrombolysis when administered in vivo in animal models of ischemic stroke.
Susan M. Shea +7 more
doaj +1 more source
Dependence of aptamer activity on opposed terminal extensions : improvement of light-regulation efficiency [PDF]
, 2009 Aptamers that can be regulated with light allow precise control of protein activity in space and time and hence of biological function in general. In a previous study, we showed that the activity of the thrombin-binding aptamer HD1 can be turned off by ...
Buff, Maximilian +6 more
core +2 more sources
Life, 2020 In the present in vitro selection study, we isolated and characterized RNA aptamers for a tRNA-binding protein (Trbp) from an extremophile archaeon Aeropyrum pernix.
Senri Ohmori +6 more
doaj +1 more source
Agreement of aptamer proteomics with standard methods for measuring venous thrombosis biomarkers
Research and Practice in Thrombosis and Haemostasis, 2021 Background Venous thromboembolism (VTE) is a complex disease with an incidence rate of about 1 in 1000 per year. Despite the availability of validated biomarkers for VTE, unprovoked events account for 50% of first events.
Tariq Faquih +5 more
doaj +1 more source