Regulation of mRNA translation by a photoriboswitch. [PDF]
, 2020 Optogenetic tools have revolutionized the study of receptor-mediated processes, but such tools are lacking for RNA-controlled systems. In particular, light-activated regulatory RNAs are needed for spatiotemporal control of gene expression.
Abdelsayed, Michael M +7 more
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Carbon nanotube decorated magnetic microspheres as an affinity matrix for biomolecules [PDF]
, 2013 Carbon nanotube (CNT) decorated magnetic microspheres were fabricated to develop a multimodal platform that utilizes non-covalent molecular interactions of CNTs to magnetically separate biomolecules.
Kolkar Mohammed, Javed Hussain Niazi +1 more
core +1 more source
Programmable biomaterials for dynamic and responsive drug delivery [PDF]
, 2016 Biomaterials are continually being designed that enable new methods for interacting dynamically with cell and tissues, in turn unlocking new capabilities in areas ranging from drug delivery to regenerative medicine. In this review, we explore some of the
Almquist, BD, Kiani, MT, Stejskalova, A
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Thermodynamic characterization of an engineered tetracycline-binding riboswitch [PDF]
, 2006 Riboswitches reflect a novel concept in gene regulation that is particularly suited for technological adaptation. Therefore, we characterized thermodynamically the ligand binding properties of a synthetic, tetracycline (tc)-binding RNA aptamer, which ...
Müller, Michael +3 more
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Probing Essential Nucleobase Functional Groups in Aptamers and Deoxyribozymes by Nucleotide Analogue Interference Mapping of DNA [PDF]
Journal of the American Chemical Society, 2011 Nucleotide analogue interference mapping of DNA (dNAIM) is here introduced as a new nonenzymatic interference-based approach that enables high-throughput identification of essential nucleobase functional groups in DNA aptamers and in the catalytic core of deoxyribozymes.
Wachowius, F., Höbartner, C.
openaire +3 more sources
Insights into ligand binding to PreQ1 Riboswitch Aptamer from molecular dynamics simulations. [PDF]
PLoS ONE, 2014 Riboswitches play roles in transcriptional or translational regulation through specific ligand binding of their aptamer domains. Although a number of ligand-bound aptamer complex structures have been solved, it is important to know ligand-free ...
Zhou Gong +4 more
doaj +1 more source
The Validation and Amplification of Random DNA Libraries with Modified Nucleobases for Click-SELEX
Biology and Life Sciences Forum, 2022 Aptamers are nucleic acid ligands which exhibit specific binding to a desired target. Oligonucleotides are limited in their ability to form hydrophobic interactions.
Natalia Komarova +2 more
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RNA-aptamers-in-droplets (RAPID) high-throughput screening for secretory phenotypes. [PDF]
, 2017 Synthetic biology and metabolic engineering seek to re-engineer microbes into living foundries for the production of high value chemicals. Through a design-build-test cycle paradigm, massive libraries of genetically engineered microbes can be constructed
Abate, Adam +8 more
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Biochemistry, 2020 The ability to change binding affinity in a controlled fashion is a key step in the rational design of biomolecules in general and functional nucleic acids in particular. Here, we use dangling nucleotides to alter the binding affinity of structure-switching aptamers.
Sladjana Slavkovic +2 more
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Next‐Generation Nucleic Acid Aptamers with Two‐Base‐Modified Nucleotides Have Improved Binding Affinity and Potency [PDF]
ChemBioChem, 2017 Twice as apt: Nucleic acid aptamers with high binding affinity, specificity, epitope coverage and nuclease resistance were developed by using libraries containing oligonucleotides in which two bases in the pyrimidine nucleotide had been modified.
Hadi, AlShamaileh, Rakesh N, Veedu
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