Aptamer fluorescence anisotropy sensors for adenosine triphosphate by comprehensive screening tetramethylrhodamine labeled nucleotides [PDF]
Biosensors and Bioelectronics, 2015 We previously reported a fluorescence anisotropy (FA) approach for small molecules using tetramethylrhodamine (TMR) labeled aptamer. It relies on target-binding induced change of intramolecular interaction between TMR and guanine (G) base. TMR-labeling sites are crucial for this approach. Only terminal ends and thymine (T) bases could be tested for TMR
Qiang, Zhao, Qin, Lv, Hailin, Wang
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Model-guided design of ligand-regulated RNAi for programmable control of gene expression [PDF]
, 2008 Progress in constructing biological networks will rely on the development of more advanced components that can be predictably modified to yield optimal system performance.
Chase L Beisel +4 more
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PLoS ONE, 2021 Oligonucleotide-based aptamers, which have a three-dimensional structure with a single-stranded fragment, feature various characteristics with respect to size, toxicity, and permeability.
Gwangho Lee +3 more
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Engineering ligand-responsive RNA controllers in yeast through the assembly of RNase III tuning modules [PDF]
, 2011 The programming of cellular networks to achieve new biological functions depends on the development of genetic tools that link the presence of a molecular signal to gene-regulatory activity.
An +37 more
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Design and clinical developments of aptamer-drug conjugates for targeted cancer therapy
Biomaterials Research, 2021 Background Aptamer has been called “chemical antibody” which displays the specific affinity to target molecules compared to that of antibodies and possesses several therapeutic advantages over antibodies in terms of size, accessibility to synthesis, and ...
Do-Hun Kim +3 more
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A blue light receptor that mediates RNA binding and translational regulation [PDF]
, 2019 Sensory photoreceptor proteins underpin light-dependent adaptations in nature and enable the optogenetic control of organismal behavior and physiology.
Bittl, Robert +9 more
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Trends in Medical Sciences, 2021 : Targeted cancer therapy is developing rapidly according to the fact that it has been demonstrated that this type of therapy can reduce various side effects and adverse events of the commonly available cancer treatment approaches such as chemotherapy and radiotherapy.
Pouya Safarzadeh Kozani +2 more
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HIV-1 reverse transcriptase mutations that confer decreased in vitro susceptibility to anti-RT DNA aptamer RT1t49 confer cross resistance to other anti-RT aptamers but not to standard RT inhibitors [PDF]
, 2005 RNA and DNA aptamers specific for HIV-1 reverse transcriptase (RT) can inhibit reverse transcription in vitro. RNA aptamers have been shown to potently block HIV-1 replication in culture.
Fisher, Timothy S +2 more
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Towards the selection of phosphorothioate aptamers [PDF]
European Journal of Biochemistry, 2000 The high affinity of a given nucleic acid for a protein ligand can be used to isolate specific inhibitors of enzymes involved in pathological situations. The latter property is the basis of the SELEX (systematic evolution of ligands by exponential enrichment) technique.
M L, Andreola +4 more
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Kinetic analysis of an efficient DNA-dependent TNA polymerase. [PDF]
, 2005 alpha-l-Threofuranosyl nucleoside triphosphates (tNTPs) are tetrafuranose nucleoside derivatives and potential progenitors of present-day beta-d-2'-deoxyribofuranosyl nucleoside triphosphates (dNTPs).
Chaput, John C +6 more
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