Results 61 to 70 of about 7,154 (227)

RNA degradomics and proteomics reveal the mechanism of dsProsβ1‐mediated proteasome targeting in Psylliodes chrysocephala

Pest Management Science, EarlyView.
RNA degradomics revealed dsProsβ1‐derived siRNA‐mediated mRNA cleavage events, mainly at uracil‐guanine and adenine‐adenine pairs. Proteasome inhibition via dsProsβ1 increased mitochondrial and cytoskeletal proteins while reducing translation‐related and mRNA‐binding proteins.
Doga Cedden, Gözde Güney, Michael Rostás, Stefan Scholten   +3 more
wiley   +1 more source

Development and application of KASP assays to differentiate between Sorghum bicolor, halepense, and their hybrids

Pest Management Science, EarlyView.
A panel showing the morphological differences between Sorghum halepense (A), hybrids (B), and Sorghum bicolor (C) with a novel KASP‐based genetic assay capable of reliably distinguishing between them. Partially Created in BioRender. https://BioRender.com/1u30ukv Abstract BACKGROUND Sorghum bicolor and Sorghum halepense can readily hybridize, creating ...
Connor Purvis, Eric L. Patterson, Erin E. Burns   +2 more
wiley   +1 more source

Gene‐specific double‐stranded RNAs induce mortality in the South African mealybug Delottococcus aberiae

Pest Management Science, EarlyView.
Gene‐specific double‐stranded RNAs effectively silence essential genes in the invasive citrus pest Delottococcus aberiae, leading to significant mortality through both microinjection and oral delivery. These results provide proof of concept for RNA interference‐based biopesticides as selective and sustainable tools for citrus integrated pest management.
Carolina Gallego‐Giraldo, Alberto Urbaneja, Omar Ruíz‐Rivero, Verónica Aragonés, Marie Guijarro‐Guijarro, José‐Antonio Daròs, Meritxell Perez‐Hedo   +6 more
wiley   +1 more source

Single and dual RPA‐CRISPR/Cas assays for point‐of‐need detection of Stewart's wilt pathogen (Pantoea stewartii subsp. stewartii) of corn and Maize dwarf mosaic virus

Pest Management Science, Volume 81, Issue 4, Page 1988-1999, April 2025.
Schematic diagram of the single and dual RPA‐CRISPR/Cas12a/13a diagnostic assays for the detection of Pantoea stewartii subsp. stewartii and Maize dwarf mosaic virus. The validated assays provide a useful and sensitive molecular tool for detecting two quarantine pathogens of maize within a minimal resource framework suitable for fast‐tracking the ...
Qian Tian, Hua Zhou, Zhenxing Zhao, Yongjiang Zhang, Wenjun Zhao, Lulu Cai, Tao Guo   +6 more
wiley   +1 more source

Detection of Leptospira in cane toads (Rhinella jimi) from urban and rural Paraíba, Brazil

Veterinary Record, EarlyView.
Abstract Background Leptospirosis is a significant zoonosis in tropical regions, where poor sanitation and favourable climate aid its spread. Synanthropic animals such as the cane toad (Rhinella jimi), which share environments with both people and wild and domestic animals, may harbour Leptospira and contribute to urban and rural transmission cycles ...
Karla N. de Souza Rocha, Aline de Lima Peixoto, Lucila S. de Medeiros Pereira, Debora Ferreira Cardoso, Maria L. C. Rodrigues Silva, Sérgio S. de Azevedo, Juliana P. F. de Oliveira, Clebert J. Alves, Mozar Alves Fonseca, Severino S. dos Santos Higino   +9 more
wiley   +1 more source

Click Conjugates of Artificial Ribonucleases: Sequence Specific Cleavage with Multiple Turnover

Chemistry – A European Journal
AbstractThe azido modified RNA cleaving catalyst 2 has been attached to oligonucleotides containing alkyne linkers in a central position. The resulting conjugates hybridize specifically with complementary RNA strands and cleave them with multiple substrate turnover. RNA half‐lives are in the range of 6–7 hours (pH 8, 37 °C).
Sandra Weber, Timo Weinrich, Ute Scheffer, Elisabeth Kalden, Michael W. Göbel   +4 more
openaire   +2 more sources

Inhibition of Targeted Bacterial Growth Using MazF‐Expressing Phagemids: A Novel Therapeutic Approach Based on Endoribonuclease Toxin Molecules

Microbiology and Immunology, EarlyView.
ABSTRACT The extensive and indiscriminate use of antibiotics, which are intended to halt bacterial growth or eliminate them, has unintentionally given rise to antimicrobial resistance (AMR), which is a critical threat to global health. In the pursuit of alternative solutions, researchers have focused on type II toxin–antitoxin systems prevalent within ...
Hana Hasegawa, Tatsuki Miyamoto, Rino Isshiki, Tomoyoshi Kaneko, Kenichi Takasugi, Naohiro Noda, Satoshi Tsuneda   +6 more
wiley   +1 more source

Silencing of juvenile hormone‐related genes through RNA interference leads to molt failure and high mortality in the spongy moth

Insect Science, EarlyView.
The feasibility of using RNA interference to control the globally important quarantine pest, the spongy moth. Targeting genes related to JHs play an important role in the growth and development of insects. First, the open reading frames (ORFs) of Ldjhamt and Ldjheh were identified and characterized, and the target genes were cloned and double‐stranded ...
Wenzhuai Ji, Qing Xie, Xiaoxiao Chang, Juan Shi   +3 more
wiley   +1 more source

DsiRNA‐mediated silencing of Ceratitis capitata transformer or transformer‐2 leads to masculinization of XX embryos and systemic gene silencing in ovaries

Insect Science, EarlyView.
We carried out functional studies using Dicer‐substrate small interfering RNAs (DsiRNAs), targeting the sex determination genes Cctransformer (Cctra) and Cctransformer‐2 (Cctra‐2) in the Mediterranean fruit fly (Ceratitis capitata). Microinjection of DsiRNAs into XX embryos (0‐1 h old) rapidly triggered the expression of male‐specific Cctra isoforms ...
Gennaro Volpe, Sarah Maria Mazzucchiello, Domenico De Falco, David Torrente, Stefania Liguori, Noemi Rosati, Dora Baccaro, Michela Mazzeo, Fulvio Bertolotto, Harshini Sangle, Ennio Giordano, Angela Carfora, Francesca Lucibelli, Marianna Varone, Paola Di Lillo, Hugo Diego Perdomo, Mariangela Bonizzoni, Serena Aceto, Marco Salvemini, Molly Duman‐Scheel, Giuseppe Saccone   +20 more
wiley   +1 more source

Dimerization‐dependent NOTCH receptor transactivation unveils a class of highly selective NOTCH signalling inhibitors

The FEBS Journal, EarlyView.
NOTCH signalling is indispensable for tissue homeostasis and, consequently, corruption of its normal function promotes numerous diseases, including cancer. However, the development of targeted therapies has been hampered by inefficacy and overt toxicity. Here, we show that NOTCH receptor dimerization is necessary for receptor transactivation, which has
Xinxin Liu, Haijiang Wang, Gunja Mishra, Lin‐Ting Wu, Chao Li, Maarten van Dinther, Jin Liu, Manuel A.F.V. Gonçalves, Peter ten Dijke, David A. Baker   +9 more
wiley   +1 more source