Results 31 to 40 of about 9,203 (183)

Boarder control on the IFT train

eLife, 2014
New details are revealed about the system that transports proteins to the tip of flagella during growth.
Cécile Fort, Philippe Bastin
doaj   +1 more source

Life-Saver or Undertaker: The Relationship between Primary Cilia and Cell Death in Vertebrate Embryonic Development

Journal of Developmental Biology, 2022
The development of multicellular organisms requires a tightly coordinated network of cellular processes and intercellular signalling. For more than 20 years, it has been known that primary cilia are deeply involved in the mediation of intercellular ...
Thorsten Pfirrmann, Christoph Gerhardt
doaj   +1 more source

IC2 participates in the cooperative activation of outer arm dynein densely attached to microtubules

Cell Structure and Function, 2023
Ciliary outer-arm dynein (OAD) consists of heavy chains (HCs), intermediate chains (ICs), and light chains (LCs), of which HCs are the motor proteins that produce force.
Yusuke Kondo, Tomoka Ogawa, Emiri Kanno, Masafumi Hirono, Takako Kato-Minoura, Ritsu Kamiya, Toshiki Yagi   +6 more
doaj   +1 more source

Second harmonic microscopy of axonemes

Optics Express, 2009
We performed Second Harmonic Microscopy of axonemes obtained from sea urchin sperm. Using polarization analysis and a trade-off between signal and photodamage, we were able to determine, for the first time to our knowledge, the nonlinear susceptibility chizxx/chixzx = 1.1+/-0.2 and chizzz/chixzx = 4+/-0.5 of axonemes.
Odin, Christophe, Heichette, Claire, Chrétien, Denis, Le Grand, Yann   +3 more
openaire   +3 more sources

Condition‐Associated Pattern Extraction and Recovery From Multi‐Condition Single‐Cell RNA‐seq Data With CAPER

Advanced Science, EarlyView.
Decoupling biological signals from unwanted variation in multi‑condition single‑cell RNA sequencing data remains challenging. CAPER disentangles condition‑associated biological effects from sample heterogeneity through matrix factorization, producing interpretable latent factors and a batch‑corrected expression matrix.
Ye Li, Jin Ning, An Wang, Minxi Shi, Yuanze Chen, Guoliang Liu, Shiquan Sun   +6 more
wiley   +1 more source

Modes of flagellar assembly in Chlamydomonas reinhardtii and Trypanosoma brucei

eLife, 2014
Defects in flagella growth are related to a number of human diseases. Central to flagellar growth is the organization of microtubules that polymerize from basal bodies to form the axoneme, which consists of hundreds of proteins.
Johanna L Höög, Sylvain Lacomble, Eileen T O’Toole, Andreas Hoenger, J Richard McIntosh, Keith Gull   +5 more
doaj   +1 more source

Molecular and Cellular Hallmarks of Age‐Related Vestibular Hair Cell Degeneration

Advanced Science, EarlyView.
This study utilizes single‐cell RNA‐seq transcriptomes, advanced imaging, and electrophysiology to examine universal and cell‐type‐specific aging signatures of vestibular hair cells. The study shows that impaired hair bundle function is a key driver of age‐related vestibular dysfunction.
Samadhi Kulasooriya, Huizhan Liu, Sarath Vijayakumar, Celia Bloom, Zhenhang Xu, Shu Tu, Benjamin J. Borgmeier, Mi Zhou, Litao Tao, Bechara Kachar, David Z. He   +10 more
wiley   +1 more source

Recombinant Monoclonal Antibodies for Detecting the Tubulin Post‐Translational Modifications Glutamylation and Lysine‐40 Acetylation

Cytoskeleton, EarlyView.
ABSTRACT Post‐translational modifications (PTMs) to tubulin subunits in microtubule filaments are thought to comprise a component of the tubulin code that specifies microtubule functions in cell physiology and animal development. Acetylation of Lysine‐40 (K40) on α‐tubulin (αTub‐K40ac) and glutamylation of both α‐ and β‐tubulin are two tubulin PTMs of ...
Lynne Blasius, Ezekiel C. Thomas, Erik H. Collet, Erin Jenson, Chad G. Pearson, Ryoma Ohi, Kristen J. Verhey   +6 more
wiley   +1 more source

Proteins with proximal-distal asymmetries in axoneme localisation control flagellum beat frequency

Nature Communications
The 9 + 2 microtubule-based axoneme within motile flagella is well known for its symmetry. However, examples of asymmetric structures and proteins asymmetrically positioned within the 9 + 2 axoneme architecture have been identified.
Cecile Fort, Benjamin J. Walker, Lore Baert, Richard J. Wheeler   +3 more
doaj   +1 more source

The inner junction complex of the cilia is an interaction hub that involves tubulin post-translational modifications

eLife, 2020
Microtubules are cytoskeletal structures involved in stability, transport and organization in the cell. The building blocks, the α- and β-tubulin heterodimers, form protofilaments that associate laterally into the hollow microtubule.
Ahmad Abdelzaher Zaki Khalifa, Muneyoshi Ichikawa, Daniel Dai, Shintaroh Kubo, Corbin Steven Black, Katya Peri, Thomas S McAlear, Simon Veyron, Shun Kai Yang, Javier Vargas, Susanne Bechstedt, Jean-François Trempe, Khanh Huy Bui   +12 more
doaj   +1 more source