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Insect Science, Volume 32, Issue 6, Page 2105-2124, December 2025.Distinct lineages of the grasshopper Chorthippus parallelus (Orthoptera: Acrididae) form a well‐known hybrid zone (HZ) in the Pyrenees mountain range; the Iberian endemism C. p. erythropus (Cpe) and the subspecies C. p. parallelus, (Cpp) widely distributed throughout the rest of Europe. Both subspecies differ in diverse traits, including the strains of
Patricia Jiménez‐Florido +4 more
wiley +1 more source
A transcriptomic atlas facilitating systems biology approaches in pea
Crop Science, Volume 65, Issue 6, November/December 2025.Abstract Pea is a model organism in biology and an agronomic crop, entering the genomics era. Although RNA‐sequencing (RNA‐seq) has become the main transcriptomic approach in biology, there is currently no genome‐wide expression atlas covering a wide range of biological conditions for this species.
Gatepe Cedoine Kodjovi +11 more
wiley +1 more source
Influence of heat stress, sex and genetic groups on reference genes stability in muscle tissue of chicken. [PDF]
PLoS ONE, 2017 Quantitative RT-PCR is an important technique for assessing gene expression. However, a proper normalization of reference genes prior to expression analyses of target genes is necessary.
Haniel Cedraz de Oliveira +5 more
doaj +1 more source
Diabetes/Metabolism Research and Reviews, Volume 41, Issue 7, October 2025.ABSTRACT Background In older adults, obesity is associated with frailty, conditions worsened by age related decline in bone mineral density (BMD) and muscle mass. Objective To evaluate whether a 3‐month Fibre‐Enriched High Carbohydrate (FEHC) diet preserves bone health, reduces inflammation and modulates Wnt signalling in older adults with obesity ...
Francesca Cannata +16 more
wiley +1 more source
BMC Research Notes, 2011 Findings At first 32 housekeeping genes were analyzed in six randomly chosen meningiomas, brain and dura mater using geNorm, NormFinder, Bestkeeper-1 software and the comparative ΔCt method.
Pfister Christina +2 more
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Molecular Ecology, Volume 34, Issue 20, October 2025.ABSTRACT Plant viruses often alter host traits in ways that affect interactions with herbivores, potentially facilitating their own acquisition and transmission by insect vectors. However, little is known about the molecular mechanisms underlying this phenomenon.
Thomas Armand +5 more
wiley +1 more source
Selection of reference genes for transcriptional analysis of edible tubers of potato (Solanum tuberosum L.). [PDF]
PLoS ONE, 2015 Potato (Solanum tuberosum) yield has increased dramatically over the last 50 years and this has been achieved by a combination of improved agronomy and biotechnology efforts.
Roberta Fogliatto Mariot +7 more
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Influence of heat stress on reference genes stability in heart and liver of two chickens genotypes.
PLoS ONE, 2020 INTRODUCTION:Real-time polymerase chain reaction (RT-qPCR) is an important tool for analyzing gene expression. However, before analyzing the expression of target genes, it is crucial to normalize the reference genes, in order to find the most stable gene
Juliana Gracielle Gonzaga Gromboni +7 more
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Validation of reference genes aiming accurate normalization of qRT-PCR data in Dendrocalamus latiflorus Munro. [PDF]
PLoS ONE, 2014 BACKGROUND: Dendrocalamus latiflorus Munro distributes widely in subtropical areas and plays vital roles as valuable natural resources. The transcriptome sequencing for D.
Mingying Liu +4 more
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Screening of Reference miRNA of Different Early- and Late-Flowering Tree Peony Varieties
Plants, 2023 miRNA plays an important role in plant growth and development and in response to various stresses. Quantitative real-time PCR (qRT-PCR) technology is often used to detect the expression level of miRNAs and genes by comparing with reference genes.
Jiajia Shen +4 more
doaj +1 more source