Results 51 to 60 of about 7,909 (199)

Determination of qPCR Reference Genes Suitable for Normalizing Gene Expression in a Canine Model of Duchenne Muscular Dystrophy [PDF]

Background:Dogs with dystrophin-deficient muscular dystrophy are valuable models of the equivalent human disease, Duchenne Muscular Dystrophy (DMD): unlike the mdx mouse, these animals present a disease severity and progression that closely matches that ...
Aartsma-Rus, Ali, Andersen, Bages, Bello, Bish, Breitbart, Brinkmeyer-Langford, Burton, Bustin, Bustin, Camerino, Cohn, Cotten, Craig, Crist, Duan, Falzarano, Feder, Fjeldbo, Flanigan, Galindo, Gong, Hildyard, Kallestad, Kornegay, Kornegay, Li, Liu, Mah, McGreevy, Nghiem, Pegoraro, Pfaffl, Piva, Reinig, Rho, Robinson-Hamm, Robriquet, Schmittgen, Solano, Spassov, Swaggart, Thellin, Vieira, Vieira Natassia, Walmsley, Webster, Wuebbles, Yu, Zammit   +50 more
core   +1 more source

Identification of Reference Genes for Reverse Transcription-Quantitative PCR Analysis of Ginger Under Abiotic Stress and for Postharvest Biology Studies

Frontiers in Plant Science, 2022
Gene expression analysis largely improves our understanding of the molecular basis underpinning various plant biological processes. Stable reference genes play a foundational role during the normalization of gene expression levels.
Gang Li, Jia-wei Ma, Junliang Yin, F. Guo, Keyong Xi, Peihua Yang, Xiaodong Cai, Qie Jia, Lulu Li, Yiqing Liu, Yongxing Zhu   +10 more
semanticscholar   +1 more source

Expression Stability of Reference Genes in Larvae of Spodoptera frugipferda Under Azadirachtin Stress by Real-Time Quantitative PCR Analysis

Guangdong nongye kexue
【Objective】Real-time quantitative PCR is one of the most conventional and efficient techniques for studying the mRNA expression level of target genes. The proper selection of reference genes is of great significance for the accuracy of the results.
Benshui SHU, Yuting HUANG, Xuanyue YU, Cuiting LIU, Xinyi XIE, Hao SHEN, Jintian LIN   +6 more
doaj   +1 more source

Evaluation and validation of reference genes for RT-qPCR gene expression in Naegleria gruberi

Scientific Reports, 2023
Naegleria gruberi is a free-living amoeboflagellate commonly found in freshwater and in soils around the world. It is a non-pathogenic relative of Naegleria fowleri, which is the etiologic agent of Primary Amoebic Meningoencephalitis (PAM).
Tania Martín-Pérez, Martina Köhsler, Julia Walochnik   +2 more
doaj   +1 more source

Selection of miRNA reference genes for plant defence studies in rice (Oryza sativa)

, 2019
RT-qPCR is a widely used method to investigate the expression levels of genes under certain conditions. A key step, however, to have reliable results is the normalization of expression.
De Meyer, Tim, De Smet, Lien, Kyndt, Tina, Verstraeten, Bruno   +3 more
core   +1 more source

Selection and evaluation of reference genes for qRT-PCR in Inonotus obliquus

Frontiers in Microbiology
Real-time quantitative reverse transcription PCR (RT-qPCR) is one of the most effective tools for studying gene expression. Inonotus obliquus is a rare and edible medicinal fungus.
Lulu Li, Xiaofan Guo, Shouming Wang
semanticscholar   +1 more source

Selection and Validation of Reliable Reference Genes for Gene Expression Studies in Different Genotypes and TRV-Infected Fruits of Peach (Prunus persica L. Batsch) during Ripening

Genes, 2022
Real-time quantitative PCR (RT-qPCR) is a powerful tool to detect and quantify transcription abundance, and the stability of the reference gene determines its success.
Ze Xu, Jieyu Dai, Weijing Su, Haixia Wu, Kamran Shah, Libo Xing, Juanjuan Ma, Dong Zhang, Caiping Zhao   +8 more
semanticscholar   +1 more source

Validation of suitable reference genes by various algorithms for gene expression analysis in Isodon rubescens under different abiotic stresses

Scientific Reports, 2022
Isodon rubescens (Hemsley) H. Hara (Lamiaceae) is a traditional Chinese medicine plant that has been used to treat various human diseases. Oridonin is one of the main active ingredients, and the route of its molecular biosynthesis remains to be ...
Conglong Lian, Bao Zhang, Jingfan Yang, Jinxu Lan, Han Yang, Kaihua Guo, Jingjing Li, Sui-qin Chen   +7 more
semanticscholar   +1 more source

Differential gene expression in Chorthippus parallelus (Zetterstedt, 1821) (Orthoptera: Acrididae: Gomphocerinae) induced by Wolbachia infection

Insect Science, Volume 32, Issue 6, Page 2105-2124, December 2025.
Distinct lineages of the grasshopper Chorthippus parallelus (Orthoptera: Acrididae) form a well‐known hybrid zone (HZ) in the Pyrenees mountain range; the Iberian endemism C. p. erythropus (Cpe) and the subspecies C. p. parallelus, (Cpp) widely distributed throughout the rest of Europe. Both subspecies differ in diverse traits, including the strains of
Patricia Jiménez‐Florido, Mónica Aquilino, David Buckley, José L. Bella, Rosario Planelló   +4 more
wiley   +1 more source

Selection and validation of reference genes suitable for gene expression analysis by Reverse Transcription Quantitative real-time PCR in Acinetobacter baumannii

Scientific Reports
Acinetobacter baumannii is a Gram-negative bacterium considered an emerging multi-drug-resistant pathogen. Furthermore, this bacterium can survive in extreme environmental conditions, which makes it a frequent cause of nosocomial infection outbreaks ...
Paloma Aparecida Alves de Oliveira, Juliana Baboghlian, Clarissa Orandina Aparecida Ramos, Alquiandra Stefani Ferreira Mançano, Andréia de Melo Porcari, Raquel Girardello, Lúcio Fábio Caldas Ferraz   +6 more
semanticscholar   +1 more source